浙江农业科学 ›› 2025, Vol. 66 ›› Issue (1): 197-200.DOI: 10.16178/j.issn.0528-9017.20240564

• 畜牧兽医 • 上一篇    下一篇

“检测淘汰法”在温州地区规模猪场动物疫病净化中的应用

吴波1(), 章国永2, 赵妍1, 麻武飞1, 周轩伦1   

  1. 1.温州市动物疫病预防控制中心,浙江 温州 325000
    2.苍南县动物疫病预防控制中心,浙江 苍南 325800
  • 收稿日期:2024-07-16 出版日期:2025-01-11 发布日期:2025-01-14
  • 作者简介:吴波(1981—),男,浙江温州人,高级兽医师,硕士,主要从事动物疫病综合防控工作,E-mail:529110315@qq.com
  • 基金资助:
    温州市农业丰收计划项目(FSJH2024036);温州市科协服务科技创新项目(KJFW2024-002)

Application of the “detection and elimination method” in the purification of animal diseases in large-scale pig farms in Wenzhou

WU Bo1(), ZHANG Guoyong2, ZHAO Yan1, MA Wufei1, ZHOU Xuanlun1   

  1. 1. Wenzhou Animal Disease Prevention and Control Center, Wenzhou 325000, Zhejiang
    2. Animal Disease Prevention and Control Center in Cangnan County, Cangnan 325800, Zhejiang
  • Received:2024-07-16 Online:2025-01-11 Published:2025-01-14

摘要:

为推动及改进规模猪场动物疫病净化,该试验以“检测淘汰法”为核心,以本底调查、免疫程序优化、免疫干扰分析和生物安全体系改进等为配套的净化集成技术,将温州一个规模猪场作为示范场点,以O型口蹄疫和非洲猪瘟为净化对象。净化实施后该猪场生产防疫指标有所改善,口蹄疫免疫抗体合格率从76.84%提高至91.09%,口蹄疫野生型抗体阳性率从4.21%降低至0,口蹄疫野生型核酸阳性率从3.68%降低至0,非洲猪瘟野生型核酸阳性率一直维持在0。在示范场跟踪监测4 a后,推广至全市10家规模猪场,O型口蹄疫免疫抗体合格率整体呈现逐年上升趋势,口蹄疫感染抗体阳性数和病毒核酸阳性数整体呈现逐年下降趋势,非洲猪瘟病毒核酸阳性数历年跟踪监测检出率均为0。

关键词: 猪, 检测淘汰法, 疫病净化, 口蹄疫, 非洲猪瘟

Abstract:

In order to promote and improve the purification of animal diseases, this study takes the “detection and elimination method” as the core, and integrates purification technologies such as background investigation, immune program optimization, immune interference analysis, and biosafety system improvement. A large-scale pig farm in Wenzhou was taken as a demonstration site, with O-type aftosa virus and African swine fever virus as purification objects. After the purification was implemented, the production and epidemic prevention indicators of the pig farm had been improved. The qualified rate of aftosa virus immune antibodies had increased from 76.84% to 91.09%, the positive rate of aftosa virus wild-type antibodies had decreased from 4.21% to 0, the positive rate of aftosa virus wild-type nucleic acid had decreased from 3.68% to 0, and the positive rate of African swine fever virus wild-type nucleic acid had remained at zero. After four years of tracking and monitoring in the demonstration site, it has been promoted to more than 10 large-scale pig farms in the city. The overall qualified rate of O-type aftosa virus immune antibodies had shown an increasing trend year by year, while the number of positive antibodies and virus nucleic acid for aftosa virus had shown an overall decreasing trend year by year. The number of positive nucleic acid tested for African swine fever virus had been tracked and monitored over the years with a zero.

Key words: pig, detection elimination method, disease purification, aftosa virus, African swine fever virus

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