稻纵卷叶螟不同发育阶段miRNA表达谱分析

doi:10.16178/j.issn.0528-9017.20240744

摘要/Abstract

摘要：

该文通过高通量测序技术分析了稻纵卷叶螟五龄幼虫和蛹中miRNA的表达情况,并对显著差异表达的miRNAs进行了靶基因的预测和功能富集分析。在稻纵卷叶螟的五龄幼虫和蛹的miRNA文库中,存在44个miRNAs。五龄幼虫和蛹文库之间差异表达显著的miRNAs有11个,对应的靶基因有756个。对靶基因进行功能分析,发现这些靶基因显著富集到14个GO条目。该研究不仅提供了稻纵卷叶螟五龄幼虫和蛹发育过程中miRNA表达谱和差异表达信息,也为miRNA调控昆虫发育的功能研究奠定了基础。

关键词: 稻纵卷叶螟, miRNA, 靶基因

Abstract:

This study analyzed the expression profiles of miRNAs in fifth-instar larvae and pupae of Cnaphalocrocis medinalis using high-throughput sequencing technology, and predicted the target genes of significantly differentially expressed miRNAs and performed functional enrichment analysis. In the miRNA library of fifth-instar larvae and pupae of C. medinalis, 44 miRNAs were present. There were 11 differentially expressed miRNAs between the fifth-instar larval and pupal libraries, with a total of 756 predicted target genes. Functional analysis of the target genes showed that these target genes were significantly enriched to 14 GO terms. This study not only provided miRNA expression profile and differential expression information during the development of fifth-instar larvae and pupae of C. medinalis, but also laid a foundation for the study of the function of miRNA in regulating insect development.

Key words: Cnaphalocrocis medinalis, miRNA, target gene

中图分类号:

S435.112+.1

朱宇, 刘洋. 稻纵卷叶螟不同发育阶段miRNA表达谱分析[J]. 浙江农业科学, 2025, 66(4): 979-985.

ZHU Yu, LIU Yang. Analysis of miRNA expression profiles at different developmental stages of Cnaphalocrocis medinalis[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(4): 979-985.

图/表 6

参考文献 41

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样品	原始序列/ 条	纯净序列的数量/ 条(比例/%)	比对到稻纵卷叶螟基因组的数量/条(比例/%)
Larvae1	12 609 492	9 937 945(78.8)	6 070 640(48.1)
Larvae2	13 822 323	10 874 477(78.7)	6 815 993(49.3)
Larvae3	14 537 527	10 860 480(74.7)	7 719 131(53.1)
Pupae1	10 273 225	8 302 666(80.8)	5 958 208(58.0)
Pupae2	12 165 146	9 960 787(81.9)	6 317 880(51.9)
Pupae3	10 156 333	8 033 670(79.1)	5 546 433(54.6)

样品	原始序列/ 条	纯净序列的数量/ 条(比例/%)	比对到稻纵卷叶螟基因组的数量/条(比例/%)
Larvae1	12 609 492	9 937 945(78.8)	6 070 640(48.1)
Larvae2	13 822 323	10 874 477(78.7)	6 815 993(49.3)
Larvae3	14 537 527	10 860 480(74.7)	7 719 131(53.1)
Pupae1	10 273 225	8 302 666(80.8)	5 958 208(58.0)
Pupae2	12 165 146	9 960 787(81.9)	6 317 880(51.9)
Pupae3	10 156 333	8 033 670(79.1)	5 546 433(54.6)

miRNA名称		5p序列		3p序列		前体序列	基因座
cme-miR-279a		aguggguguaagucuggaagcaca		ugacuagaucuacacucauuga		aguggguguaagucuggaagcacaguguucaauucugugacuagaucuacacucauuga	Chr21:6202860..6202919:+
cme-miR-9		ucuuugguauccuagcuguagg		uaaaguuaugguaccgaaguua		ucuuugguauccuagcuguaggcguauugagcgaacccuaaaguuaugguaccgaaguua	Chr10:16800953..16801013:-
cme-miR-276		agcgagguauagaguuccuacg		uaggaacuucauaccgugcucu		agcgagguauagaguuccuacguaucauuaacuguaggaacuucauaccgugcucu	Chr15:12727857..12727913:-
cme-bantam		ugguuuucauaaugauuugacaga		ugagaucauugugaaagcugau		ugguuuucauaaugauuugacagauuguuuucaauauucugagaucauugugaaagcugau	Chr15:9669491..9669552:+
cme-miR-279c		gaugagugaaucucuaguucaag		ugacuagauuuucacuuauccu		gaugagugaaucucuaguucaagucucuccguaucuugacuagauuuucacuuauccu	Chr12:14524777..14524835:+
cme-miR-305		auuguacuucaucaggugcucugg		aggcgcuuguuggaguacacuua		auuguacuucaucaggugcucuggugaugaucguuccaggcgcuuguuggaguacacuua	Chr10:120298..120358:+
cme-miR-279b		gaugaguggagguuuagugcaug		ugacuagauccacacucaucca		gaugaguggagguuuagugcauguuucuguacaucaugacuagauccacacucaucca	Chr4:6349550..6349608:-
miRNA名称	5p序列		3p序列		前体序列			基因座
cme-miR-2	ucggcaaagcggcugugcugug		uaucacagccagcuuuguugacu		ucggcaaagcggcugugcugugcuguuccauagacauaucacagccagcuuuguugacu			Chr1:6383303..6383362:-
cme-miR-13	ucguaaaaaugguuguguuuug		uaucacagccauuuuugacgagu		ucguaaaaaugguuguguuuuguagauucaucauaucacagccauuuuugacgagu			Chr1:6383573..6383629:-
cme-miR-71	ugaaagacuuggguagugagaug		ucucacuaccuugucuuucaug		ugaaagacuuggguagugagauguccugcacaucacaaauucucacuaccuugucuuucaug			Chr1:6409942..6410004:-

miRNA名称		5p序列		3p序列		前体序列	基因座
cme-miR-279a		aguggguguaagucuggaagcaca		ugacuagaucuacacucauuga		aguggguguaagucuggaagcacaguguucaauucugugacuagaucuacacucauuga	Chr21:6202860..6202919:+
cme-miR-9		ucuuugguauccuagcuguagg		uaaaguuaugguaccgaaguua		ucuuugguauccuagcuguaggcguauugagcgaacccuaaaguuaugguaccgaaguua	Chr10:16800953..16801013:-
cme-miR-276		agcgagguauagaguuccuacg		uaggaacuucauaccgugcucu		agcgagguauagaguuccuacguaucauuaacuguaggaacuucauaccgugcucu	Chr15:12727857..12727913:-
cme-bantam		ugguuuucauaaugauuugacaga		ugagaucauugugaaagcugau		ugguuuucauaaugauuugacagauuguuuucaauauucugagaucauugugaaagcugau	Chr15:9669491..9669552:+
cme-miR-279c		gaugagugaaucucuaguucaag		ugacuagauuuucacuuauccu		gaugagugaaucucuaguucaagucucuccguaucuugacuagauuuucacuuauccu	Chr12:14524777..14524835:+
cme-miR-305		auuguacuucaucaggugcucugg		aggcgcuuguuggaguacacuua		auuguacuucaucaggugcucuggugaugaucguuccaggcgcuuguuggaguacacuua	Chr10:120298..120358:+
cme-miR-279b		gaugaguggagguuuagugcaug		ugacuagauccacacucaucca		gaugaguggagguuuagugcauguuucuguacaucaugacuagauccacacucaucca	Chr4:6349550..6349608:-
miRNA名称	5p序列		3p序列		前体序列			基因座
cme-miR-2	ucggcaaagcggcugugcugug		uaucacagccagcuuuguugacu		ucggcaaagcggcugugcugugcuguuccauagacauaucacagccagcuuuguugacu			Chr1:6383303..6383362:-
cme-miR-13	ucguaaaaaugguuguguuuug		uaucacagccauuuuugacgagu		ucguaaaaaugguuguguuuuguagauucaucauaucacagccauuuuugacgagu			Chr1:6383573..6383629:-
cme-miR-71	ugaaagacuuggguagugagaug		ucucacuaccuugucuuucaug		ugaaagacuuggguagugagauguccugcacaucacaaauucucacuaccuugucuuucaug			Chr1:6409942..6410004:-

miRNA名称	5p序列	3p序列	前体序列	基因座
cme-nov-1	cuaccgacugaacagacuaggu	cuagucucuuucgucgguaaau	cuaccgacugaacagacuaggucucccgacauaccuagucuc uuucgucgguaaau	Chr15:1596464..1596520:+
cme-nov-11	ugggcuccggcaacgucgagua	uucgacguugccguagucuacu	ugggcuccggcaacgucgaguacguagcucggugagcguugu cuauucgacguugccguagucuacu	Chr1:5638030..5638097:+
cme-nov-14	auuuucacacacgauugaauc	gacggucgugugugaaagcga	auuuucacacacgauugaaucuaguuaccuucugagacggu cgugugugaaagcga	Chr1:15273400..15273456:+
cme-nov-15	cagaguauugcagaugaaacga	guuucaucugcaauacucuacg	cagaguauugcagaugaaacgacgaaaaaaucuuguuucau cugcaauacucuacg	Chr9:9395051..9395107:-
cme-nov-16	gagcggauaacauucggaacga	guuccgaauuuuauccgcuagg	gagcggauaacauucggaacgauguuucucuagcggauaau uuccguuccgaauuuuauccgcuagg	Chr17:4724197..4724264:-
cme-nov-17	uggugcuuguaacuucuuugua	caaggaaguuacaagcaccaac	uggugcuuguaacuucuuuguaaguuccaauccaaggaagu uacaagcaccaac	Chr28:3976039..3976093:+
cme-nov-18-1	cuuugaaccgcugcucuaaca	cuagcgcagcgguuuaaagcu	cuuugaaccgcugcucuaacacgggccucuuguuugcuagc gcagcgguuuaaagcu	Chr19:10350876..10350933:-
cme-nov-19	ucacacaggucaagccggcgccuu	gggccggcuugaccugaguggca	ucacacaggucaagccggcgccuucauucggacaagggccgg cuugaccugaguggca	Chr14:2737359..2737417:-
cme-nov-18-2	cuuugaaccgcugcacuaaca	cuagcgcagcgguuuaaagcu	cuuugaaccgcugcacuaacaugggucuuuuguuugcuagc gcagcgguuuaaagcu	Chr19:10350335..10350392:-
cme-nov-2	ucacacaggucaagcaggcgccuu	gggccggcuugaccugaguggca	ucacacaggucaagcaggcgccuucauucggacaagggccg gcuugaccugaguggca	Chr14:2733471..2733529:-