浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (4): 1001-1004.DOI: 10.16178/j.issn.0528-9017.20240961

• 植保技术 •    

4种病原线虫对蛹期甜菜夜蛾的致病力评价

刘敏1(), 林雅1,2, 章金明1,*()   

  1. 1.浙江省农业科学院 植物保护与微生物研究所,浙江 杭州 310021
    2.浙江师范大学 化学与生命科学学院,浙江 金华 321004
  • 收稿日期:2024-12-16 出版日期:2025-04-11 发布日期:2025-05-09
  • 通讯作者: 章金明
  • 作者简介:章金明(1975—),男,浙江新昌人,副研究员,从事外来入侵物种及经济作物害虫监测预警及综合治理研究,E-mail:zhanginsect@163.com。
    刘敏(1981—),女,安徽阜阳人,实验师,主要从事重大害虫监测预警及绿色防控技术研究,E-mail:liumin198110@163.com。
  • 基金资助:
    浙江省重点研发“领雁”计划项目(2022C02030);浙江省“三农六方”科技协作计划(2020SNLF023)

Pathogenicity evaluation of four pathogenic nematodes on Spodoptera exigua at pupal stage

LIU Min1(), LIN Ya1,2, ZHANG Jinming1,*()   

  1. 1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang
    2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang
  • Received:2024-12-16 Online:2025-04-11 Published:2025-05-09
  • Contact: ZHANG Jinming

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摘要： 为筛选防治甜菜夜蛾的优良昆虫病原线虫品系,本研究以室内生测的方式评价并比较了4种昆虫病原线虫品系对甜菜夜蛾预蛹和蛹的致病力,同时评估了土壤含水量对线虫致病力的影响。结果表明,4种昆虫病原线虫对甜菜夜蛾预蛹和蛹均有一定的致病力,且其对甜菜夜蛾蛹的致病力低于预蛹。当线虫浓度相同时,斯氏线虫(Steinernema carpocapsae)All、X-7和JY-90品系对甜菜夜蛾预蛹的致死效果均优于异小杆线虫(Heterorhabditis indica)LN2品系。当线虫浓度为100 IJs·头-1时,斯氏线虫X-7、JY-90和All品系对甜菜夜蛾预蛹的致死率均超过80%,异小杆线虫LN2品系的致死率仅为40%左右。当线虫浓度为1 600 IJs·头-1时,斯氏线虫X-7、JY-90和All品系对甜菜夜蛾蛹的致死率分别为67.5%、40.0%、18.7%,而异小杆线虫LN2品系对甜菜夜蛾蛹的致死率仅为38.6%。试验进一步评估了15%和20%土壤含水量对X-7品系线虫致死率的影响,发现在含水量20%的土壤中X-7品系对甜菜夜蛾蛹的致死率更高,说明昆虫病原线虫在甜菜夜蛾的防治中具有良好的应用前景。

关键词: 昆虫病原线虫, 甜菜夜蛾, 蛹, 预蛹, 致病力

Abstract:

The pathogenicity of four entomopathogenic nematodes (EPNs) against the prepupae and pupae of Spodoptera exigua was tested in laboratory to screen the effective strains of EPNs for the control of S. exigua, and the effect of soil moisture on the pathogenicity of EPNs was evaluated. Results showed that four EPNs exhibited different pathogenicity to S. exigua at the prepupa and pupa stages. At the same dose, the lethal effects of All, X-7, and JY-90 strains of Steinernema carpocapsae on the prepupa of S. exigua were better than that of the LN2 strain of Heterorhabditis indica. When the dose of EPNs was 100 IJs per prepupa, the mortality of the X-7, JY-90, and All strains of S. carpocapsae exceeded 80%, whereas the mortality of the LN2 strain of H. indica was only about 40%. The pathogenicity of the four EPNs to S. exigua pupae was lower than that to the prepupae. When the dose of EPNs was 1 600 IJs per pupa, the mortality of the X-7, JY-90, and All strains of S. carpocapsae were 67.5%, 40.0%, 18.7%, respectively, and the mortality of the LN2 strain of H. indica was only 38.6%. The effects of two soil moisture levels, 15% and 20%, on the mortality rate of the X-7 strain were evaluated, with the X-7 strain exhibiting a higher mortality rate for pupae in soil with 20% moisture content, indicating that entomopathogenic nematodes have a good application prospect in the control of S. exigua.

Key words: entomopathogenic nematodes, Spodoptera exigua, prepupa pupa, pathogenicity

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