Introduction performance of japonica soft rice variety Zhehexiang 2 in Zhejiang Province

doi:10.16178/j.issn.0528-9017.20230970

Abstract

Abstract:

Zhehexiang 2 is a single season conventional late japonica rice variety developed in collaboration with Jiaxing Academy of Agricultural Sciences, Zhejiang Dahe Seed Industry Co., Ltd., and Zhejiang Wuwangnong Seed Shareholding Co., Ltd. In 2021, initially the experiment was conducted in Zhejiang Province and simultaneously the molecular characteristics analysis was conducted using molecular markers. The results showed that the average yield of Zhehexiang 2 was 9 664.5 kg·hm^-2, which increased by 5.8% compared with the control Xiushui 134. And its growth period was 160 days, which was 3.0 days more than the control variety. Moreover, this variety carries rice blast resistance genes, including Pi36, Pi50, Pizt, Pi63, Pib, Pi64, Pish, Pi37, Ptr, Pikm2 and Pi35. The sequence analysis revealed that there was a 7 bp missing at the 7th exon of the rice aroma regulating gene. Compared with the soft rice variety Nanjing 46, there is a G/T mutation in the first base of the Wx gene in Zhehexiang 2. These results will provide a theoretical foundation for the promotion and application of this variety and further genetic improvement.

Key words: rice, variety characteristics, introduction, high quality resistance to rice blast, molecular characteristics

CLC Number:

S512

HOU Fan, SHEN Fengping, SUN Yiming, CHEN Youyuan, ZHAN Liwei. Introduction performance of japonica soft rice variety Zhehexiang 2 in Zhejiang Province[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(4): 790-794.

Figures/Tables 8

References 10

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基因	上游引物	下游引物	片段大小
Pib	GAACAATGCCCAAACTTGAGA	GGGTCCACATGTCAGTGAGC	365(R)
Pi2	CAGCGATGGTATGAGCACAA	CGTTCCTATACTGCCACATCG	450(R)/282(S)
Pi9	GCTGTGCTCCAAATGAGGAT	GCGATCTCACATCCTTTGCT	397(S)/291(R)
Pi-t	ATGATAACCTCATCCTCAATAAGT	GTTGGAGCTACGGTTGTTCAG	733(R)
Pi-ta	AGCAGGTTATAAGCTAGGCC	CTACCAACAAGTTCATCAAA	1042(R)
Pi56	TGCAAGAACCCTCCTCCTAC	CCTGCCATCTCAAGACTCTC	725(S)
Pid4	CCGCATCTCCTCCTTCCTCT	AGAGATCTCGCTGTTCAGTC	352(S)/279(R)
Pikm2	CAGTAGCTGTGTCTCAGAACTATG	AAGGTACCTCTTTTCGGCCAG	332(S)/290(R)
fgr(测序)	GTTGTTGTTGCTGCTGTTTT	TTGCAAACTAAACCCTTGAG
Wx-1(测序)	CTACAAATAGCCACCCACAC	ACCCTCCTGATCTGTTTCTC
Wx-2(测序)	GGTCATGGTGATCTCTCCTC	GTTGTTCTTCAGGTAGCTCG
Wx-3(测序)	CAAGGACAAGTACATCACCG	TTCTCCTCCATGCTCTTGAG

基因	上游引物	下游引物	片段大小
Pib	GAACAATGCCCAAACTTGAGA	GGGTCCACATGTCAGTGAGC	365(R)
Pi2	CAGCGATGGTATGAGCACAA	CGTTCCTATACTGCCACATCG	450(R)/282(S)
Pi9	GCTGTGCTCCAAATGAGGAT	GCGATCTCACATCCTTTGCT	397(S)/291(R)
Pi-t	ATGATAACCTCATCCTCAATAAGT	GTTGGAGCTACGGTTGTTCAG	733(R)
Pi-ta	AGCAGGTTATAAGCTAGGCC	CTACCAACAAGTTCATCAAA	1042(R)
Pi56	TGCAAGAACCCTCCTCCTAC	CCTGCCATCTCAAGACTCTC	725(S)
Pid4	CCGCATCTCCTCCTTCCTCT	AGAGATCTCGCTGTTCAGTC	352(S)/279(R)
Pikm2	CAGTAGCTGTGTCTCAGAACTATG	AAGGTACCTCTTTTCGGCCAG	332(S)/290(R)
fgr(测序)	GTTGTTGTTGCTGCTGTTTT	TTGCAAACTAAACCCTTGAG
Wx-1(测序)	CTACAAATAGCCACCCACAC	ACCCTCCTGATCTGTTTCTC
Wx-2(测序)	GGTCATGGTGATCTCTCCTC	GTTGTTCTTCAGGTAGCTCG
Wx-3(测序)	CAAGGACAAGTACATCACCG	TTCTCCTCCATGCTCTTGAG

地点	播种期(月-日)	移栽期(月-日)	始穗期(月-日)	齐穗期(月-日)	成熟期(月-日)	全生育期/d	秀水134全生育期/d
嘉善	06-08	06-03	09-04	09-06	11-01	146	141
余姚	05-21	06-17	09-02	09-06	11-03	166	163
萧山	05-19	06-12	08-29	08-31	11-04	168	163
德清	05-21	06-23	09-01	09-04	10-30	162	157
诸暨	05-25	06-18	08-31	09-02	10-28	156	159
平均						160	157

地点	播种期(月-日)	移栽期(月-日)	始穗期(月-日)	齐穗期(月-日)	成熟期(月-日)	全生育期/d	秀水134全生育期/d
嘉善	06-08	06-03	09-04	09-06	11-01	146	141
余姚	05-21	06-17	09-02	09-06	11-03	166	163
萧山	05-19	06-12	08-29	08-31	11-04	168	163
德清	05-21	06-23	09-01	09-04	10-30	162	157
诸暨	05-25	06-18	08-31	09-02	10-28	156	159
平均						160	157

品种	试验点	有效穗/ (万·hm^-2)	株高/ cm	每穗总粒数	每穗实粒数	结实率/ %	千粒重/ g	产量/ (kg·hm^-2)
浙禾香2号	嘉善	270.8	97.1	127.6	116.4	91.2	26.6	9 651.0
	余姚	346.5	92.0	145.0	131.0	92.2	25.7	10 749.0
	萧山	287.9	96.9	171.1	160.1	93.6	25.3	8 422.5
	诸暨	292.5	97.0	144.0	123.7	85.9	27.3	9 372.0
	德清	321.0	95.0	138.5	125.6	90.7	25.3	10 131.0
	平均	303.7	95.6	145.2	131.4	90.4	26.0	9 665.1
秀水134	嘉善	243.8	92.6	144.9	137.4	94.8	27.7	9 054.0
	余姚	331.5	92.0	149.1	137.5	90.3	25.5	10 209.0
	萧山	263.9	89.7	171.2	162.0	94.6	25.9	8 175.0
	诸暨	274.5	92.0	147.3	131.0	88.9	25.3	8 838.0
	德清	307.5	94.0	138.4	123.6	89.3	25.1	9 412.5
	平均	284.2	92.1	150.2	138.3	91.6	25.9	9 137.7