Abstract:

This study utilized stem segments of Sedum formosanum N.E.Br. as experimental materials. Sterile seedlings were obtained through pretreatment and sterilization of explants. Subsequently, the explants were transferred to different proliferation and rooting media for gradient experiments, followed by comparative analysis to screen the optimal tissue culture formulation. The results showed that spraying the mother plants with 800-1 000 times diluted 50% carbendazim solution every other day in one week prior to explant collection effectively reduced endophytic bacteria and improved the survival rate of sterile explants. The highest sterile survival rate of explants (76%) was achieved by adding 0.2% plant tissue culture antimicrobial agent to the induction medium, combined with sterilization using alcohol for 30 s and 0.1% HgCl₂ for 8 min. The proliferation medium MS + 1.5 mg·L^-1 6-BA + 0.1 mg·L^-1 NAA + 30 g·L^-1 sucrose + 8 g·L^-1 agar demonstrated superior proliferation effects, with a proliferation coefficient of 21.4. Sedum formosanum N.E.Br. exhibited excellent rooting performance on 1/2 MS medium, achieving a 100% rooting rate. This study established a tissue culture rapid propagation system for Sedum formosanum N.E.Br., laying a foundation for its industrialized production and enriching China's groundcover plant species resources.

Key words: Sedum formosanum N.E.Br., fungicides, groundcover