Identification and fungicide screening of the pathogen of gray mold on Fritillaria thunbergii

doi:10.16178/j.issn.0528-9017.20240206

Abstract

Abstract:

Taking gray mold plants of Fritillaria thunbergii as the materials, using tissue separation, pathogenicity determination, the pathogens of gray mold on F. thunbergii was identified and the effects of chemical fungicides on the pathogen were studied. The results showed that the pathogens of gray mold on F. thunbergii belongs to Botrytis spp., which is the most closely related to B. tulipae. The optimal temperature of the isolate BMY was 20 ℃; the optimal pH value was 4-8; and the optimal carbon and nitrogen sources were fructose and beef extract, respectively. The toxicity measurement showed that 430 g·L^-1 tebuconazole SC and 500 g·L^-1 fluazinam SC had strong inhibitory effect on the pathogen BMY, with EC₅₀ values of 5.100 9 and 4.084 2 μg·mL^-1, respectively, which could be used as candidate agents. In this study, the pathogens of gray mold on F. thunbergii were clarified, and two fungicides with good inhibition effect were screened, which could provide a reference for the field prevention and control of gray mold on F. thunbergii.

Key words: Fritillaria thunbergii, gray mold, biological characteristics

CLC Number:

S567.231

WU Jun, XIE Yunye, FANG Li, WANG Hanrong. Identification and fungicide screening of the pathogen of gray mold on Fritillaria thunbergii[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(5): 1210-1215.

Figures/Tables 5

References 21

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序列	GenBank号
序列	ITS	gpd	hsp60	rpb2
B. cinerea SAS56	AJ716294	AJ705006	AJ716067	AJ745677
B.cinerea SAS405	—	AJ705005	AJ716066	AJ745678
B.elliptica BE9610	—	AJ705011	AJ716072	AJ745683
B.elliptica BE9714	AJ716300	AJ705012	AJ716073	AJ745684
B.globosa MUCL444	—	AJ705022	AJ716083	AJ745693
B.narcissicola MUCL18857	—	AJ705025	AJ716086	AJ745698
B.narcissicola MUCL2120	—	AJ705026	AJ716087	AJ745697
B.polyblastis CBS287.38	AJ716291	AJ705030	AJ716091	AJ745702
B.sphaerosperma MUCL21481	AJ716293	AJ705035	AJ716096	AJ745708
B.ulipae Bt9001	—	AJ705040	AJ716101	AJ745712
B.tulipae Bt9830	AJ716301	AJ705041	AJ715102	AJ745713
B.tulipae Bt9901		AJ705042	AJ716103	AJ745714
Monilinia fructigena 9201	—	AJ705043	AJ716047	AJ745715
Sclerotinia sclerotiorum 484	—	AJ705044	AJ716048	AJ745716
BMY	MZ722984	MZ770838	MZ770852	MZ770846
BM11	MZ722986	MZ770839	MZ770853	MZ770847
BM20	MZ722985	—	MZ770854	MZ770848
BM22	MZ722987	MZ770840	MZ770855	MZ770849
BM31	MZ722988	MZ770841	MZ770856	MZ770850
BM42	MZ723908	MZ770842	MZ770857	MZ770851

序列	GenBank号
序列	ITS	gpd	hsp60	rpb2
B. cinerea SAS56	AJ716294	AJ705006	AJ716067	AJ745677
B.cinerea SAS405	—	AJ705005	AJ716066	AJ745678
B.elliptica BE9610	—	AJ705011	AJ716072	AJ745683
B.elliptica BE9714	AJ716300	AJ705012	AJ716073	AJ745684
B.globosa MUCL444	—	AJ705022	AJ716083	AJ745693
B.narcissicola MUCL18857	—	AJ705025	AJ716086	AJ745698
B.narcissicola MUCL2120	—	AJ705026	AJ716087	AJ745697
B.polyblastis CBS287.38	AJ716291	AJ705030	AJ716091	AJ745702
B.sphaerosperma MUCL21481	AJ716293	AJ705035	AJ716096	AJ745708
B.ulipae Bt9001	—	AJ705040	AJ716101	AJ745712
B.tulipae Bt9830	AJ716301	AJ705041	AJ715102	AJ745713
B.tulipae Bt9901		AJ705042	AJ716103	AJ745714
Monilinia fructigena 9201	—	AJ705043	AJ716047	AJ745715
Sclerotinia sclerotiorum 484	—	AJ705044	AJ716048	AJ745716
BMY	MZ722984	MZ770838	MZ770852	MZ770846
BM11	MZ722986	MZ770839	MZ770853	MZ770847
BM20	MZ722985	—	MZ770854	MZ770848
BM22	MZ722987	MZ770840	MZ770855	MZ770849
BM31	MZ722988	MZ770841	MZ770856	MZ770850
BM42	MZ723908	MZ770842	MZ770857	MZ770851

杀菌剂	毒力回归方程	EC₅₀/(μg·mL^-1)	相关系数
氟啶胺	Y=6.467 9x-0.118 3	4.084 2	0.951 2
戊唑醇	Y=7.462 6x-0.202 1	5.100 9	0.987 6
氟菌·肟菌酯	Y=7.143 2x-0.215 6	48.304 2	0.974 7
吡唑醚菌酯	Y=5.812 9x-0.131 3	2 047.70	0.971 4
啶酰菌胺	Y=5.641 4x-0.208 7	46 261.20	0.941 3

杀菌剂	毒力回归方程	EC₅₀/(μg·mL^-1)	相关系数
氟啶胺	Y=6.467 9x-0.118 3	4.084 2	0.951 2
戊唑醇	Y=7.462 6x-0.202 1	5.100 9	0.987 6
氟菌·肟菌酯	Y=7.143 2x-0.215 6	48.304 2	0.974 7
吡唑醚菌酯	Y=5.812 9x-0.131 3	2 047.70	0.971 4
啶酰菌胺	Y=5.641 4x-0.208 7	46 261.20	0.941 3