Pathogen identification, biological characteristics and fungicides screening of leaf spot disease of Saccharum officinarum

doi:10.16178/j.issn.0528-9017.20240266

Abstract

Abstract:

The diseased leaves and stems of Saccharum officinarum were collected from Wenling County, Taizhou City, Zhejiang Province. The results showed that the pathogens were identified as Curvularia lunata based on pathogen isolation, Koch postulates and molecular identification. The effect of carbon source, nitrogen source, temperature, light and pH value on growth of GZWB21 were determined, these results suggested that strain GZWB21 grew well on six carbon sources, the optimal carbon source and nitrogen source of GZWB21 were sucrose and yeast powder, respectively. The optimal temperature was 35℃, the optimal pH value was 8. Besides, 43% fluoromystrobin suspension, 450 g·L^-1 prochloraz aqueous emulsion and 50% acetimidine aqueous dispersible granules had a strong inhibitory effect on GZWB21, with EC₅₀ of 0.003 8、0.002 7 and 0.124 0 mg·L^-1. This study can provide a reference for the prevention and control of leaf spot disease of Saccharum officinarum.

Key words: Saccharum officinarum, leaf spot, pathogen, biological characteristics, fungicides

CLC Number:

S435.661

ZHANG Yuqing, WU Jun, FANG Li, JIN Wenxuan, WANG Hanrong. Pathogen identification, biological characteristics and fungicides screening of leaf spot disease of Saccharum officinarum[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(6): 1429-1432.

Figures/Tables 6

References 12

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温度/℃	菌落直径/cm	pH值	菌落直径/cm
25	2.80 ± 0.46 a	5	4.43 ± 0.24 ab
30	3.17 ± 0.58 a	6	4.58 ± 0.05 ab
35	3.28 ± 0.62 a	7	4.51 ± 0.16 ab
40	0.50 b	8	4.78 ± 0.05 a
		9	4.38 ± 0.02 b
		10	3.81 ± 0.05 c

温度/℃	菌落直径/cm	pH值	菌落直径/cm
25	2.80 ± 0.46 a	5	4.43 ± 0.24 ab
30	3.17 ± 0.58 a	6	4.58 ± 0.05 ab
35	3.28 ± 0.62 a	7	4.51 ± 0.16 ab
40	0.50 b	8	4.78 ± 0.05 a
		9	4.38 ± 0.02 b
		10	3.81 ± 0.05 c

光照	菌落直径/cm
连续光照	6.38 ± 0.17 a
连续黑暗	5.83 ± 0.22 ab
光暗交替	5.80 ± 0.16 b

光照	菌落直径/cm
连续光照	6.38 ± 0.17 a
连续黑暗	5.83 ± 0.22 ab
光暗交替	5.80 ± 0.16 b

碳源	菌落直径/cm	氮源	菌落直径/cm
蔗糖	4.72 ± 0.08 a	酵母粉	4.58 ± 0.05 a
肌醇	4.52 ± 0.17 a	胰蛋白胨	4.22 ± 0.19 b
葡萄糖	4.17 ± 0.23 b	硝酸钾	3.62 ± 0.13 c
可溶性淀粉	4.03 ± 0.08 b	牛肉粉	3.48 ± 0.19 c
木糖	4.00 ± 0.04 b	硫酸铵	3.35 ± 0.00 cd
果糖	3.95 ± 0.22 b	L-丙氨酸	2.70 ± 0.04 e
对照(CK)	2.82 ± 0.17 c	对照(CK)	3.10 ± 0.19 e