Study on microorganisms in aquaculture water，soil，and intestine of Rana nigromaculata in the ecological breeding model

doi:10.16178/j.issn.0528-9017.20240868

Abstract

Abstract:

Rice-frog ecological breeding is one of the new breeding models actively promoted in Zhejiang Province. This study analyzed the microbial community abundance，diversity，and composition of aquaculture water，soil，and intestine of Rana nigromaculata under three different breeding densities（20 000，25 000，and 30 000 frogs per 667 m2）during two key farming periods（August and October）. The results showed that the microbial community abundance of aquaculture water，soil，and intestine of Rana nigromaculata were higher in October than in August. The phylum with the highest relative abundance of aquaculture water，soil，and intestine of Rana nigromaculata in October were Proteobacteria，Bacteroidota，and Firmicutes，respectively. Compared with rice monoculture（control group），the microbial community abundance of aquaculture water decreased and the microbial community abundance of soil（except for high-density group） increased after rice-frog mixed breeding，the relative abundance of Actinobacteriota of aquaculture water decreased，while the relative abundance of Bacteroidota，Firmicutes，Cloacibacterium，and Comamonas of aquaculture water increased. In the high-density group，the microbial community abundance of aquaculture water，soil，and intestine of Rana nigromaculata was the lowest，while in the medium density group，the microbial community abundance of aquaculture water，soil，and intestine of Rana nigromaculata was the highest（except for the control group）. With the increase in breeding density，the relative abundance of Proteobacteria，Verrucomicrobiota and Akkermansia increased，while the relative abundance of Bacteroidota decreased in intestine of Rana nigromaculata in October，indicating that the breeding density affects microbial composition of aquaculture water，soil，and intestine of Rana nigromaculata. Appropriate breeding density can increase the microbial community abundance in Rana nigromaculata intestine，which is beneficial for maintaining intestine health.

Key words: Rana nigromaculata, breeding density, rice-frog ecological breeding, gut microbiota, soil, aquaculture water

CLC Number:

S966.3

HE Runzhen, LIANG Qianrong, ZHU Ningyu, YUAN Fan, JIN Jing, BEI Yijiang, ZHOU Fan, DING Xueyan. Study on microorganisms in aquaculture water，soil，and intestine of Rana nigromaculata in the ecological breeding model[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(3): 750-756.

Figures/Tables 9

Fig.1 Alpha diversity of intestinal microbiota in Rana nigromaculata

Fig.2 Composition of intestinal microbiota in Rana nigromaculata at the phylum level

Fig. 3 Composition of intestinal microbiota in Rana nigromaculata at the genus level

Fig.4 Alpha diversity of microorganisms in the aquaculture water of Rana nigromaculata

Fig.5 Composition of microbial communities at the phylum level in the aquaculture water of Rana nigromaculata

Fig.6 Composition of microbial communities at the genus level in the aquaculture water of Rana nigromaculata

Fig.7 Alpha diversity of microorganisms in the soil of Rana nigromaculata farming

Fig.8 Soil microbial composition at the phylum level

Fig.9 Soil microbial compositionat the genus level

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