Bioinformatics and expression pattern analysis of DoGSTF11 in Dendrobium officinale

doi:10.16178/j.issn.0528-9017.20240951

Abstract

Abstract:

Glutathione S-transferases （GST） are widely distributed in plants，and they participate in the transport of secondary metabolites in plants，the detoxification of heterologous substances，hormone regulation and cell apoptosis，et al. In this study，we analyzed one selected GST gene named DoGSTF11 through bioinformatics and expression pattern analysis. The results showed that DoGSTF11 gene encoded 221 amino acids. The relative molecular weight of DoGSTF11 protein was 25.16 ku，and its theoretical isoelectric point（pI）was 6.34. The DoGSTF11 protein was an unstable hydrophobic protein，with no transmembrane domain or signal peptide. It contained 10 protein phosphorylation sites and featured two conserved domains：N-terminal domain and C-terminal domain. Amino acid sequence analysis showed that DoGSTF11 protein had the highest homology with VvGST4 protein of V. vinifera，with a similarity of 67.0%. Tissue-specific expression analysis showed that the relative expression levels of DoGSTF11 gene varied across different strains as follows：purplish stem > pink stem > green stem，and across different tissues as follows：flower > leaf > stem > root. The results of cis-acting element analysis showed that the promoter sequence of DoGSTF11 gene contained multiple elements，which were related to plant hormones and environmental factors. The expression of DoGSTF11 gene was significantly（p<0.05） up-regulated under the induction of low temperature，which suggested that this gene may regulate the low temperature response process of Dendrobiumofficinale. This study provides a theoretical basis for further exploration of the function of DoGSTF11 gene and its role in response to abiotic stress.

Key words: Dendrobium officinale Kimura et Migo, DoGSTF11 gene, bioinformatics analysis, expression

CLC Number:

S567

ZHANG Yiqun, CHEN Jiadong, WU Yongqiang, SONG Minquan, LIU Yingying, DUAN Xiaojing, TAO Zhengming, JIANG Wu. Bioinformatics and expression pattern analysis of DoGSTF11 in Dendrobium officinale[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(3): 634-639.

Figures/Tables 7

Fig.1 Conservative domain of DoGSTF11 protein

Fig.2 Prediction analysis of DoGSTF11 protein

Fig.3 Bioinformatics analysis of DoGSTF11 protein

Table 1 Cis-acting elements of DoGSTF11 gene promoter

元件名称	序列（5′→3′）	数量	功能
CAAT-box	CAAAT	21	启动子和增强子区域共有的顺式作用元件
TATA-box	TATATAAA	9	转录起始-30区的核心启动子元件
P-box	CCTTTTG	1	GA₃响应
LTR	CCGAAA	1	低温响应
TC-rich repeats	ATTCTCTAAC	2	胁迫响应
TCT-motif	TCTTAC	1	部分光响应

Fig.4 Tissue-specific expression of the DoGSTF11 gene

Fig.5 Expression of DoGSTF11 in different strains

Fig.6 Expression of DoGSTF11 under low temperature treatment

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