In order to select high-quality and high-yield new varieties of Dendrobium officinale suitable for promotion in Zhejiang Province, and promote the sustainable and healthy development of the Dendrobium officinale industry. We selected the high-quality wild Dendrobium officinale germplasm from the Yandang Mountain as experimental materials. After years of self pollination and breeding, the excellent strain Yanhu 1 was selected from its offspring seedlings. Continuous regional trials of agronomic traits, yield, and quality of varieties was conducted from 2021 to 2024. The results showed that there were significant (p<0.05) differences between Yanhu 1 the control variety and Xianhu 1 (Zhejiang Medicine No. 2008003) in both stem diameter and stem fresh weight. Among them, Yanhu 1 had an average stem diameter of 8.01 mm, which was significantly higher than the control variety Xianhu 1 by 37.39%. In terms of yield, compared with Xianhu 1, Yanhu 1 in 20 and 30 months of growth showed significant improvements in both stem and leaf fresh weight. In 30 months of growth, the average stem fresh weight reached 1 400 g·m-2, with a highly significant (p<0.01) difference. In a growth period of 20 and 30 months, the average total yield of Yanhu 1 was 875.5 and 2 796.3 g·m-2, which was equivalent to 583.96 kg and 1 865.13 kg (calculated as 667 m2), respectively, higher than the control variety Xianhu 1. In terms of quality, the various component detection indicators of Yanhu 1 complied with the provisions of the Pharmacopoeia (2020 Edition). When the growth period was 20 months and 30 months, the polysaccharide content of Yanhu 1 was 40.26% and 41.99%, respectively, which were higher than the provisions of the Pharmacopoeia of the People's Republic of China: Part 1(2020 Edition), and increased by 10.08, 12.94 percentage points compared with Xianhu 1. The experimental results indicated that Yanhu 1 has better agronomic traits, higher yield, better quality, and higher medicinal value, which can be used as an excellent new variety of Dendrobium officinale for promotion and planting throughout the province.