We described chemical composition in Cangzhu in recent years， volatile oil is the important chemical composition， The β -eudesmol， hinesol are active ingredient in volatile oil and there are 38 kind of glycosides. At the same time， we overview the applying of RAPD technology in atractylodes lancea. The results is that there are correlation in chemical composition， genetic differentiation and geographical distribution， there is some truth in bounded by a territorial division of the north-south Cangzhu， and genetic differentiation has been happened in atractylodes lancea to adapting the environment. We described advances of pharmacological in dampness spleen， cardiovascular system， genitourinary system， nervous system， and the results show that there are pharmacological activity in digestive system， cardiovascular system， genitourinary system of atractylodes lancea.

对近年来苍术所含化学成分研究进行阐述，苍术中主要化学成分研究主要集中在挥发油成分，其挥发油中含有有效成分 β-桉叶醇( β-eudesmol)、茅术醇(hinesol)，苍术中还含有38种苷类化合物，同时归纳总结了近年来分子生药学RAPD技术在苍术方面的研究进展，研究结果显示苍术的化学成分、遗传分化和地理分布有一定的相关性，认为以地域为界划分南北苍术有一定道理且由于常年适应环境的需要茅苍术已发生遗传上的分化；系统的总结了苍术提取物在燥湿健脾、心血管系统、泌尿生殖系统、神经系统等药理学研究内容，研究结果显示茅苍术提取物在消化系统、心血管系统、泌尿生殖系统等方面具有药理活性。

To study the variation rules and classify the chemotype of Atractylodes lancea(Thunb.) DC. essential oil, we collected A. lancea essential oil data from the literature referring the bioinformatics methods first. Then essential oil among different populations or different individuals in the same population was compared. It was found that, 1) there were large varieties in A. lancea essential oil among populations or even among the individuals in the same population, which involve components and its content. 2) total essential oil and the 6 major components in it (elemol, atractylon, hinesol, β-eudesmol, Selina-4(14)selina, 7(11)-dienone and atractylodin) varied continuously in different geographical population. Most of the 6 major components in 26 populations increased or decreased gradually from one population to the other except atractylon and Selina-4(14)selina, 7(11)-dienone, which were not detected in some population. 3) total essential oil of A. lancea decreased from south to north China. Since the continuous variety, the chemotype of A. lancea essential oil was classified great differently by different researcher. Cluster analysis and principal component analysis were used to classify the chemotype of A. lancea essential oil in this study after continuous variation was found. And it showed that there were mainly two chemotypes of A. lancea essential oil in China. one was Hubei chemotype (HBA), which distributed in Hubei, Anhui, Shanxi and south of Henan province, and had hinesol and β-eudesmol as the main components, with a little atractylodin and almost without atractylon and selina-4(14)selina 7(11)-dienone; the other was Mount. Maoshan Chemotype (MA), which distributed in the north of Jiangsu, Shangdong, Hebei and north of Henan provines, and characterized by atractylon and atractylodin as main components but a little hinesol, β-eudesmol and other components.A. lancea in Mount. Maoshan Jiangsu province were the representation of MA. The total essential oil content of MA was significantly lower than that of HBA. A. lancea in Mount. Dabieshan in Hubei province was due to the HBA, but they had a little unique in the essential oil from the HBA. The essential oil of individuals varied distinctly in Mount. Dabieshan population, for example, the content ratio of hinesol, β-eudesmol, and atractylodin in different indeviduals from the largest to the least were 4.57, 9.87 and 26.48, respectively. It seemed that the great variation in essential oil had some relation to the high genetic biodiversity. This paper proposed that people should pay more attention to the Mount. Dabieshan A. lancea for the biodiversity conservation.

借鉴生物信息学的思路，收集文献报道的数据资料，在分析不同产地间及同一产地不同个体间苍术挥发油变异的基础上，总结苍术种内挥发油变异特点和规律，并使用聚类分析、主成分分析等多变量分析方法探索苍术种内化学型的划分。结果表明，不同产地及同一产地不同个体间苍术挥发油均有很大变异，苍术挥发油组分及含量变异与地理分布有一定相关性，挥发油中6个主要组分含量从南向北方呈现连续递减。苍术挥发油主要呈现两种化学型，一种是以湖北苍术为代表，主要位于湖北、安徽、陕西、河南南部等地，以茅术醇、(－桉油醇为主要组成，不含或含极微量苍术酮和芹烷二烯酮，简称湖北苍术型（HBA）；另一种主要以江苏茅山为代表，主要位于江苏、山东、河北、河南北部等北方地区，主要有苍术酮、苍术素为主组成，简称茅山苍术型（MA）。

目的 探讨茅苍术提取物对胃癌细胞株BGC-823和SGC-7901的增殖抑制效应.方法 采用不同浓度(0.625,1.25,2.55,10 mg·mL-1)茅苍术水提物处理胃癌BGC-823和SGC-7901细胞,采用四甲基偶氮唑盐(MTT)法检测药物对细胞的抑制作用；倒置显微镜下观 察细胞的形态学改变；Hoechst 33342染色,荧光显微镜观察细胞凋亡形态；应用流式细胞仪观察凋亡过程中的细胞周期变化.结果 茅苍术提取物对胃癌BGC-823和SGC-7901细胞增殖均有明显的抑制作用,且呈浓度和时间依赖性；光镜下可见凋亡细胞的形态学特征性改变；流式细 胞仪检测表明经茅苍术提取物处理,BGC-823细胞周期阻滞于S期,SGC-7901细胞周期阻滞于G0/G1期.结论 体外实验显示茅苍术提取物能抑制胃癌BGC-823和SGC-7901细胞增殖,可以诱导胃癌细胞凋亡.

正胃溃疡主要表现为饱胀嗳气、饥饿不适、泛酸,易并发穿孔、出血、瘢痕性幽门狭窄及梗阻,还可并发癌变,癌变率为1.5%~2.5%[1]。目前认为,胃溃疡是由于胃黏膜的侵袭因素(胃酸、胃蛋白酶及黏膜刺激物等)和防卫因素(胃黏液层、黏膜血流、前列腺素(PGE)、表皮生长因子(EGF)及上皮细胞更新等)之间失衡所致[2]。胃溃疡属中医"痞满"、"胃脘痛"等范畴,以肝脾胃为主要病变脏腑,病机为肝失疏泄、

正苍术味辛性温,专入脾胃,具有健脾燥湿、祛风散寒、明目之功,中医临床使用历史悠久,用于脘腹胀痛、湿阻脾胃、泄泻、脚气痿蹙、风湿痹痛、风寒感冒、夜盲等证。此外,

目的 利用中药血清药物化学及血清药理学方法,探讨茅苍术保护心肌细胞氧化损伤的物质基础.方法 测定和比较茅苍术提取物及其灌胃给药前后大鼠空白和含药血清中的入血成分,然后体外培养H9c2大鼠心肌细胞,建立H2O2氧化损伤模型,以细胞形态、 LDH释放量、SOD活力、MDA水平及细胞相对凋亡情况为指标,考察茅苍术提取物及其含药血清对心肌细胞损伤的影响.结果 茅苍术提取物在血清中出现11个移行成分,其中7个为原型成分,另外4个可能为代谢产物.H2O2损伤组与正常对照组相比,细胞皱缩并且变圆,数量明显减 少,LDH释放量及MDA水平明显升高,SOD活性降低,细胞凋亡率增加;与H2O2损伤组相比,阳性Vc对照组、茅苍术提取物及其含药血清作用组的细胞 数目增多,LDH释放量和MDA水平均有不同程度的降低,SOD活性升高,凋亡细胞数目减少.结论 茅苍术提取物的11个入血成分可能为茅苍术保护心肌细胞氧化损伤的物质基础.

以百合灰霉病高抗品种索蚌和感病品种法兰西为材料,测定了2个品种在接种灰霉病菌和没接种条件下百合叶片中的可溶性蛋白、叶绿素及可溶性糖含量的动态变化,结果表明:索蚌和法兰西品种在接种灰霉病菌48 h后植株体内的蛋白质含量均低于不接种的对照,但高感品种法兰西可溶性蛋白含量的变化幅度比高抗品种索蚌显著;叶绿素含量变化的测定结果表明,索蚌叶片中总叶绿素的含量变化差异不显著,最大降幅为8.87%,叶绿素a的变化趋势与总叶绿素含量相似,而叶绿素b的含量在接种后与接种前相比有明显增加;高感品种法兰西接种后总叶绿素含量呈明显下降趋势,最大降幅为30.09%,叶绿素a的下降趋势与总叶绿素含量的下降趋势较一致,叶绿素b与接种前相比也略有下降,抗病品种索蚌的叶绿素被破坏程度低于感病品种法兰西;可溶性糖含量的变化结果表明,在没接种灰霉病菌条件下,高抗品种索蚌叶片中的可溶性糖含量均高于高感品种法兰西,差异显著,接种灰霉病病菌后2种百合叶片的可溶性糖含量均高于没接种的对照,法兰西品种可溶性糖的增加量明显高于索蚌。表明索蚌对灰霉病的抗性可能与其体内较低的含糖量有关,灰霉病菌侵染后通过影响糖类的合成,增加了叶片的糖含量,感病品种法兰西的增加量较高,受影响较大。

为了提高北苍术种子的成株率，降低种植成本，扩大种植面积，提高品质，分别进行了种子处理、土壤处理、覆盖方式及药剂处理等试验研究。试验结果表明：70%丙森锌 WP500倍液处理的存苗率和亩产量最好，存苗率45.77%，亩产量175.8kg；80%多菌灵 WP 处理土壤的田间存苗率一直保持最高，且亩产量也最高；EM菌液200倍液浸种2h 的存苗率最高，为62.34%；播后覆盖火肥且盖草的存苗率最高为81.1%，产量最高为129.1kg/ m2。