This paper is mainly presented for studying on the origin and evol-ution of rapeseeds,with sprecial reference to the historic investigation ofthe origin of Chinese Brassica cam pestris and B.juncea,and also about theintroduction of B.napus abroad.As mountain-form Pak-Choi and wild-typeblack mustard(

摘　要： 对367份榨菜和雪菜等芥菜类种质资源进行田间自然发病鉴定，筛选出对病毒病表现为免疫的材料20份。表现为高抗的材料30份，表现为抗病的材料29份。对96份自交3代以上材料苗期进行芜菁花叶病毒人工接种鉴定，筛选出11份抗病毒病材料，其中品种编号为06191和06222的2份榨菜对病毒病表现高抗，病情指数分别为3．13和3．57。06222田间鉴定和苗期接种鉴定均表现为高抗。芥菜病毒病抗源材料的筛选为抗病育种工作奠定了基础。

对6个春榨菜品种植物学和瘤状茎的性状比较研究结果表明,甬榨2号植株生长势强,瘤状茎经济性状好,且适应能力较强,可以在生产上推广应用。

采用RAPD分子标记, 对西藏地区部分白菜型和芥菜型油菜资源的遗传多样性进行了分析, 结果显示, 22条引物在106份白菜型油菜中共扩增出236条带, 多态性位点比率为89%; 24条引物在50份芥菜型油菜中共扩增出276条带, 多态性位点比率为94%. 通过UPGMA聚类分析, 将西藏白菜型油菜分为Ⅰ和Ⅱ类群; 芥菜型油菜分为Ⅰ、Ⅱ、Ⅲ和Ⅳ类群

利用SSR分子标记技术分析了23份来自西藏不同地区的芥菜型油菜材料的遗传多样性及其地理分布的相关性.结果表明,基于遗传距离聚类法可将23份供试材料分为3大类群,品种的地理来源同聚类结果具有一定的相关性;13对SSR引物在23份供试材料中共获得58条清晰可辨的条带,其中54条具有多态性,多态性位点比率为93.1%,平均每对SSR引物检测出4.3条条带,其中3.7条条带具有多态性,表现出较高的遗传多样性;23份西藏芥菜类型油菜材料间的遗传相似系数在0.79～0.99,平均值为0.89;野生芥菜型-2与芥菜型油菜-2、野生芥菜型-5与下生分枝野生油菜之间的相似性系数最低,分别为0.79和0.80. Abstract： The genetic diversity and geographical distribution correlation of 23 B. juncea L. rapeseed materials from different areas of Tibet were analyzed by using 13 SSR molecular marker technology. The results showed that 23 test materials could be divided into 3 major groups based on genetic distance clustering method. The geographical origin of eultivars had certain relationship with the clustering results. 58 clear bands were obtained from 23 test materials by using 13 pairs of SSR primers. Among them, 54 bands was polymorphic, the ratio of polymorphic sites was 93.1 %. 4.3 bands were detected by each pair of SSR primer on the average and 3.7 bands were polymorphic with higher genetic diversity. The genetic similarity coefficient among 23 B. juncea L. rapeseed materials in Tibet ranged from 0.79 to 0.99, with the average of 0. 89. The similarity coefficients between wild B. juncea L. -2 and B. juncea L. -2, between wild B. juncea L. -5 and down-branching B. juncea L. were the lowest,being 0.79 and 0. 80 respectively.

利用10对AFLP引物及13对SSR引物,分析了43份来自西藏地区部分野生类型油菜种质的遗传多样性。10对AFLP引物共得到276条清晰的谱带,其中多态性带214条,多态性位点比率为77.5%,平均每对AFLP引物得到21.4条多态性带。13对SSR引物共扩增出57条带,其中多态性带51条,多态性位点比率为89.5%,说明西藏野生油菜遗传多样性丰富。通过对西藏野生类型油菜资源的遗传距离以及聚类分析,可将西藏野生类油菜分为两大类群,分别为白菜型和芥菜型野生油菜种质资源;两种分子标记适合揭示西藏野生油菜的遗传多样性。

Genetic diversity of 73 mustard (Brassica juncea L.) accessions from Tibet Autonomous Region of China and 35 from southwest of China，northwest of China and India were analyzed using 8 SRAP (sequence－related amplified polymorphism) primers combinations on 20 phenotypic traits. SRAP results showed that a total of 139 bands were produced with polymorphism rate of 24.5%. Clustering result of SRAP showed that 108 accessions were divided into 4 groups, and the accessions in each group were mainly from the same region. Genetic diversity index of China Tibet accessions was higher than others. Variation degree of 8 important phenotypic traits of Tibet accessions showed the highest variation coefficiency was 41.29% on silique number per plant. Clustering analysis of phenotypic traits showed that 108 materials were clearly divided into different groups based on their original regions except the accessions from Northwest. In conclusion, Tibet mustard had abundant genetic diversity. The order of genetic diversity indexes from top to bottom was as follows: China Tibet, Southwestern China, Northwestern China and India. Mustard genetic diversity was mainly associated with geological and biological conditions. 

利用8对SRAP (sequence－related amplified polymorphism) 引物，结合20个表型性状对73份中国西藏及35份包含中国西南地区、西北地区和印度的芥菜型油菜进行了遗传多样性分析。8对SRAP引物共扩增出139个带谱，34个多态性条带，多态性带的比例为24.5%；SRAP分子标记结果的聚类分析将材料分为4个大类，材料按地区聚集现象明显；中国西藏地区材料的遗传多样性指数最高。对西藏材料的8个重要表型性状的变异程度分析显示，变异系数最高的性状为全株角果数，达41.29%。表型性状聚类结果表明除中国西北材料外，其它材料按地区聚集现象明显。综合分子标记和表型性状分析结果表明，中国西藏芥菜型油菜具有丰富的遗传多样性，其程度与地理环境和气候条件有十分重要的关系。不同地区芥菜型油菜遗传多样性程度从大到小依次为：中国西藏、中国西南地区、中国西北地区、印度。  

【Objective】Rapeseed(B.juncea L.) local germplasm resources would be conserved and utilized in rapeseed breeding more effectively, the genetic diversity in them was studied on molecular level instead of apparent genetics level. 【Method】73 resources in B.juncea L. from Sichuan or other regions were selected, the genetic relationship among them was studied using RAPD marker cluster analyzing and botanical trait cluster analyzing. 119 RAPD primers were used and 25 main botanical traits or 88 trait grades genetic stably were observed.【Results】The tested resources were divided into three groups and 13 units in the RAPD marker cluster analyzing. Qiubeiheiyoucai from Yunnan was the first group, more likely a special original resource. The majority resources in the second group were come from Yunnan. The majority resources in the third group were come from Sichuan(including Chongqing). The second group was divided into two sub-groups. The majority resources in one were come from Guizhou and the majority resources in the other were come from Guizhou and Yunnan. The third group were also divided into two sub-groups. The majority resources in one were come from the regions out of Sichuan and the majority resources in the other were come from Sichuan. The tested resources were divided into six groups and 28 units in the botanical trait cluster analyzing. The resources in the first group were come from Sichuan(including Chongqing). The resources in the second group were come from Sichuan and Guizhou. The majority resources in the third group were come from Yunnan-Guizhou plateau and Qinghai-Xizang plateau. The majority resources in the fourth group were come from Liangshan region in Sichuan and Yunnan-Guizhou plateau. B.carinata L. come from Ethiopia and Changningbendihuangyoucai come from Sichuan were the fifth group. The majority resources in the sixth group were come from Eastern Sichuan, Liangshan region in Sichuan and Yunnan-Guizhou plateau.【Conclusion】The different between resources in B.juncea L. from Sichuan and from Yunnan,Guizhou and other regions was obvious. There was abundant genetic diversity in resources in B.juncea L. from Sichuan. The genetic relationship among resources from different ecological regions or resources from the same ecological regions was near or far, decided by the specific ecological environment and the behavior of human. The genetic relationship and the genetic distance could be identified by RAPD marker cluster analyzing or botanical trait cluster analyzing. But the results from RAPD marker cluster analyzing were more reliable.

【目的】研究中国西南地区丰富的芥菜型油菜资源遗传多样性，为芥菜型油菜资源的保护和育种利用提供有益的数据。【方法】选取以西南地区为主的73份芥菜型油菜资源，分别进行14个随机引物RAPD标记多态性分析结果的聚类分析和15个主要植物学性状量测数据的聚类分析。【结果】RAPD标记多态性分析结果的聚类分析显示为3大类，共15个亚类：四川盆地资源为第1大类，含川东、川南、川北和重庆来源等6个亚类；盆周及盆周高原和云南部分资源为第2大类，分川东南、川西南和川西北来源等4个亚类；云贵高原及长江两岸为第3大类，以贵州、云南来源的资源为主，分贵阳周边、金沙江流域和云南中部来源等5个亚类。植物学性状量测数据的聚类分析显示为4大类，共14个亚类：第1大类以川东南、重庆和云南来源的资源为主，分3个亚类；第2大类以川南、川北、云南和贵州来源的资源为主，分4个亚类；第3大类以贵州、四川来源的资源为主，分4个亚类；第4大类以川西北来源的资源为主，含3个亚类。【结论】西南地区芥菜型油菜资源间差异明显，具有丰富的遗传多样性；RAPD标记多态性分析结果和植物学性状量测数据的聚类分析结果，显示分类主要遵循地域和生态环境规律，可分清芥菜型油菜资源间的亲缘关系和遗传距离远近；在分子水平上，分类结果可能更深入和准确可靠。

A better understanding of genetic diversity and its distribution are essential for its conservation and use. The research of it will help us to determin what and where to be conserved, and improve our understanding of the taxonomy, origin and evolution of plant species. The genetic diversities of 108 accessions including 101 entries of Brassica juncea from western China, 2 from Australia, 4 entries of B. rapa , and 1 entiry of Eruca sativa Mill.were analysed by SRAP with 23 pairs of primer combinations, AFLP with 11 primer combinations, and SSR with 10 pairs of primer combinations. The results showed that totally 313 loci were detected in these materials. The genetic similarity coefficients of 108 accessions varied from 0.378–0.936, while 103 accessions of B. juncea from 0.545–0.936. The clustering analysis indicated that the genetic similarity coefficients of 5 checks including B. rapa , Eruca sativa Mill., were less than 0.558. At the point of genetic similarity coefficient, 0.700, the 103 accessions of B. juncea were divided into 5 groups, those were group A from Yunnan-Guizhou and Southern Shaanxi, group B from Guanzhong of Shaanxi, group C and group D from Xinjiang, and group E from western China. Groups A and B were winter type, groups C, D, and E were spring type. The genetic difference among the accessions in group A was the largest, and higher than that in group B. The accessions from Shaanxi and Xinjiang were distributed into 3 groups respectively, and showed abundant genetic diversity. Group E including the most spring accessions, was divided into 3 sub-groups. The accessions in the sub-group I were from Tibet, with the genetic similarity coefficient higher than 0.83, belonging to an independent genetic system with narrow genetic background. The accessions of yellow mustard in the sub-group II were from Northern Shaanxi, showed higher genetic diversity and belonged to another independent genetic system. In the sub-group III, two accessions from Australia were similar to the spring type in China. Therefore the genetic differences in B. juncea were mainly related to geological and biological conditions. The genetic diversities in winter type of B. juncea were higher than those in spring type in China. The genetic background of B. juncea in Shaanxi and Xinjiang was wide.

利用23对SRAP引物、11对AFLP引物和10对SSR引物对我国西部地区的芥菜型油菜及其近缘种108份材料进行了遗传多样性分析, 共检测到313个等位变异, 3种标记的每对引物平均分别可检测到6.8、12.5和1.9个等位变异。包括白菜型和芸芥在内的108份品种间遗传相似系数在0.378~0.936之间, 103份芥菜型油菜品种间遗传相似系数在0.545~0.936之间。聚类分析结果表明, 在相似系数0.558处, 5个参照品种白菜型油菜、小白菜以及芸芥首先被聚出芥菜型油菜之外; 在相似系数0.70处, 103份芥菜型油菜可分为云贵陕南冬播(A)、关中冬播(B)、新疆I (C)、新疆II (D)和西部春播(E)五个类群, 其中A、B基本为冬播品种, C、D、E为春播品种。A类群品种间遗传差异最大, B类群其次。陕西和新疆的品种均分别被聚到3个类群, 表现出更广泛的遗传多样性。春播类型绝大部分被聚到E类群, E类群可分为3个亚类, 其中陕北及其邻近一带春播黄芥为一类, 形成一个独立的遗传群体, 群内遗传多样性较高; 西藏的10个品种为一类, 相似系数高达0.83以上, 表现出西藏品种遗传系统的独立和遗传基础的单一; 澳大利亚2个品种单独为一类, 与我国的春播品种关系较近。由此说明, 地理和生态条件是影响芥菜型油菜类群的主要因素, 我国的冬播品种间的遗传多样性高于春播品种, 陕西和新疆的芥菜型油菜遗传多样性较高。

摘　要： 利用ＲＡＰＤ技术对芥菜１６个变种的遗传变异进行研究。从６０个随机引物中筛选出２７个有效引物，共扩增出３３６条ＤＮＡ带，其中２７５条为多态性带，占８１．８５％，平均每个引物扩增的ＤＮＡ带数为１２．４４条。利用１９个有效引物扩增的２４０条ＤＮＡ带对芥菜１６个变种间的亲缘关系进行ＵＰＧＡ聚类分析，计算出１６个变种间的平均遗传距离为７．３４，在此基础上建立了中国菜用芥菜１６个变种的ＤＮＡ分子系统树图。该系

  为了解不同类型芥菜变种间的遗传多样性,利用66对SSR引物对16份芥菜变种进行检测。结果表明,共扩出602个条带,多态性条带占92.5%,多态信息量(PIC)变幅为0.56~0.97,平均值为0.91。遗传相似系数为0.41~0.75,平均值为0.60,当相似系数为0.61时,可将供试材料分成4类。薹芥和抱子芥均单独为一类,第3类包括笋子芥、小叶芥和叶瘤芥,第4类包括白花芥、宽柄芥、长柄芥、大叶芥、凤尾芥、分蘖芥、卷心芥、茎瘤芥、花叶芥、结球芥和大头芥。表明16个芥菜变种间存在一定的遗传多样性。通过主成分分析也将供试材料分成4大类群,2种聚类分析结果基本一致,都说明来自相同省份的绝大部分材料聚在一起,呈现出一定的地域性分布规律,但聚类结果与芥菜形态没有必然联系。 

[目的]用SSR分子标记研究三峡库区芥菜型油菜品种的遗传多样性.[方法] 使用9对SSR引物,对10份来自三峡库区的芥菜型油菜品种进行遗传多样性分析.[结果] 聚类结果表明,在遗传距离0.47处可将供试材料分为3类.[结论]地理和生态条件是影响三峡库区芥菜型油菜类群的主要因素.

芥菜(Brassica juncea Czem.et Coss)是我国原产的一类蔬菜作物。近一个世纪以来,人们对芥菜的分类进行了长期研究,但观点不一,存在较大的争议。本研究借助RAPD标记和ISSR标记对28份芥菜材料的遗传多样性以及亲缘关系进行了分析和评价。实验结果表明,本次实验所采用的两种分子标记对28份芥菜材料检测出了不同的多态性水平。主要结果如下: 1.建立了适于芥菜基因组DNA提取的方法:采用幼叶作为试验材料,采用改良CTAB法,即在液氮研磨过程中加入PVP粉末,以防止酚类物质氧化,获得了高质量的芥菜基因组DNA。 2.优化并建立了芥菜RAPD和ISSR反应体系。20μL RAPD反应体系中,模板DNA40ng,引物10pmol/μL,10×反应缓冲液,dNTPs为0.3mmol/L,TaqDNA聚合酶1U,Mg~(2+)2.0mmol/L,并进一步进行梯度退火实验,找到了芥菜RAPD最适的退火温度为38℃。20μL ISSR反应体系中,模板DNA40ng,引物10pmol/μL,10×反应缓冲液,dNTPs为0.5mmol/L,TaqDNA聚合酶1U,Mg~(2+)2.0mmol/L,找到了芥菜ISSR最适的退火温度为51℃。 3.从300条RAPD引物中筛选出20条引物分别对28份芥菜基因组DNA进行PCR扩增,共扩增出162条清晰的谱带,其中140条显示多态性,平均每个引物扩增出7.0条多态性谱带。从50条ISSR引物中筛选出18条引物分别对28份芥菜基因组DNA进行PCR扩增,共扩增出143条清晰的谱带,其中124条显示多态性,平均每个引物扩增出6.9条多态性谱带。 4.基于RAPD数据估计芥菜种质材料的遗传相似系数(GS)结果,28份材料之间的GS值在0.55～0.96之间,平均为0.72,由供试材料RAPD分子标记数据的相似系数聚类分析所绘出的树状聚类,当GS=0.72～0.76时,可将28份芥菜种质划分为三大类。基于ISSR数据估计芥菜种质材料的遗传相似系数(GS)结果,28份材料之间的GS值在0.57～0.96之间,平均为0.74,由供试材料ISSR分子标记数据的相似系数聚类分析所绘出的树状聚类图,当GS=0.72～0.76时,可将28份芥菜种质划分为三大类。 5.基于RAPD和ISSR混合数据估计芥菜种质的遗传相似系数(GS)结果,28份芥菜材料之间的相似系数值在0.58～0.97之间,平均为0.76,由供试材料的RAPD利ISSR两种实验方法的数据混合后的相似系数聚类分析所绘出的树状聚类图,当GS=0.72～0.64时,可将28份芥菜种质划分为三大类。这与RAPD和ISSR分析的结果甚为相似,但比较而言,与ISSR分析的结果更为相近。 本实验表明,不同的分子标记对相同的作物群体揭示出的遗传多样性水平和变异水平有差异。但是无论RAPD标记还是ISSR标记结果都揭示出芥菜有较高的变异水平和丰富的多样性。我们得出以下结论:(1)芥菜的遗传多样性水平和变异水平很高。(2)RAPD和ISSR分子标记技术在亲缘关系较接近的芥菜材料间的遗传多样性评价中十分有效。不同的分子标记对相同遗传群体的遗传变异和多样性水平的分子检测能力不同。并且,将不同的分子标记的基础数据合并,通过联合数据去建立亲缘关系系统树,可能会比单个分子标记对群体的遗传多样性以及亲缘关系的评价更具有客观性。

采用形态学方法和SSR分子标 记技术,对甘蓝型杂交油菜贵杂4号及其亲本进行杂种性状表现、DNA指纹图谱和种子纯度的研究结果表明:田间形态学观测结果杂种性状表现亲子间存在明显亲 子关系和母本效应,利用作物的这一共性特点可对作物种子真实性和纯度进行鉴定;采用SSR分析从100对SSR引物中筛选出5对多态性很强且极易识别的特 殊引物,通过这5对特殊引物的指纹组合构建了贵杂4号及其亲本的DNA指纹图谱;利用遗传决定的蛋白质组分不受环境影响和其在组分上的差异性反映的是基因 型的原理,通过特殊引物组合对种子的扩增图,可快速有效的对种子真实性和纯度进行鉴定。

不结球白菜(Brassica capmestris ssp. chinensis Makino)又称小白菜、青菜,北方称油菜,起源于中国,是十字花科芸薹种(Brassica capmestris L.)的1个亚种。近年来,不仅北方大量引种栽培,东南亚、日本、美国及欧洲一些国家也广泛引种,已逐渐成为世界性的蔬菜。本研究一方面对不结球白菜新种质细胞质雄性不育系P70-203与其回交父本的植物学特性进行比较分析,另一方面用分子标记技术对不结球白菜雄性不育系P70-203细胞质类型进行鉴定,及杂种一代及进行了纯度鉴定。加快不结球白菜育种进程,为其进一步应用提供了分子依据。 1.不结球白菜细胞质雄性不育系P70-203是本所在育种过程中发现的半不育株,后经回交选育而成。本研究对其植物学特性进行观察测定,结果表明：不育系P70-203生长整齐,植株健壮,幼苗生长正常,不表现黄化,且不育性稳定,不育率不育度均为100%；其花朵开放正常、雌蕊功能健全、雄蕊退化、无花药；蜜腺发育良好、花蜜多、正常能吸引蜜蜂等昆虫正常采蜜传粉；具有优良的植物学性状。以上研究为配制更多优良杂种一代,推广不育系P70-203的应用范围奠定基础。 2.根据OguraCMS、PolimaCMS的不育性状相关的线粒体基因序列设计特异引物,对不结球白菜雄性不育系新种质P70-203及其保持系P60-27-1进行PCR分析。研究结果表明,Polima引物P3／P4,P5／P6在不育系与可育系中均无扩增条带；Ogura引物P1／P2在不育系中扩增出750bp的特异片段,但可育系中无扩增条带。将扩增的特异条带回收并测序,将得到的测序结果在NCBI中进行Blastn同源性比较,结果与青花菜Ogura细胞质雄性不育同源性等均达到99%。从分子角度初步推测：该雄性不育系新种质P70-203具有Ogura (?)田胞质。 3.采用RAPD、SRAP、SSR三种标记技术,对不结球白菜杂交一代08-1-61及双亲进行指纹图谱鉴定。对160个RAPD引物、132对SRAP引物筛选、87对SSR引物筛选结果表明：1条RAPD引物、1对SRAP引物在杂交种扩增双亲的互补特征带,有效地鉴别双亲和杂种F1；2条RAPD引物、1对SRAP引物为偏母型；1条RAPD与1对SRAP引物为偏父型；未筛选出呈多态性的SSR引物。三种标记比较分析,RAPD标记扩增图谱条带较为丰富,其成本低,操作简便,但重复性较差；SSR标记重复性最好,但多态性最低,成本高；SRAP标记扩增图谱条带最丰富,多态性高,重复性好,是种子指纹图谱鉴定的优良手段。

利用SSR分子标记技术对43份贵杂系列甘蓝型杂交油菜品种(组合)及亲本材料进行研究,筛选出了一批在贵杂系列亲本和杂种上具有较高多态性的SSR引物,构建了贵杂系列油菜亲本及杂交组合的指纹图谱.通过对24份甘蓝型油菜的SSR分析,从100对SSR引物中筛选到21对多态性很强且极易识别的引物,从中筛选出4对核心引物(Na12C06、Na10B04b、0110A05、Na10D03),构建了贵杂系列常用油菜亲本的指纹图谱;对这些亲本组配的19个杂交组合进行SSR分析,从100对引物中筛选到22对适合油菜杂交种的多态性引物,从中选取4对核心引物(Na12C06、Na10D03、Na10E02c、0110A05),构建了贵杂系列常用油菜杂交组合的指纹图谱;利用核心引物组合扩增的方法,可以鉴定油菜杂交组合及其亲本的真实性和纯度.

利用SSR分子标记对收集来自全国的15份茎瘤芥材料进行了检测,结果表明,79对引物共扩增出633个条带,扩增条带变幅在3~14之间,平均每对引物扩增出8.01个,其中多态性条带共478条,占75.51%。Shannon多态性指数变幅为0.72~3.72,平均值为2.77;15份茎瘤芥材料间的遗传相似系数变幅在0.60~0.75之间,平均值为0.68。在相似系数为0.70处,可将供试材料分为6类。基于SSR分子标记的主成分分析将供试材料分为5个类。说明大部分供试材料间相对遗传距离较大,亲缘关系较远,遗传多样性比较丰富。

To understand the cytoplasmic diversity of B. oleracea and B. juncea , 36 accessions including 15 B. oleracea , 12 B. juncea , 4 B. napus accessions representing different cytoplasm types, 2 B. rapa, 1 B. nigra , 1 B. carinata and 1 Eruca sativa Mill, were analyzed by using the mitochondria and chloroplast-specific SSR markers. Seven of 18 chloroplast specific SSR primers could detect 31 polymorphic bands in total. The mean allele number per locus was 4.43 and the mean of PIC was 0.550 per locus. Two mitochondria-specific SSR primers generated three and five polymorphic bands and the PIC was 0.409 and 0.558 respectively. The genetic similarity coefficients were from 0.538 to 1.000 in 36 accessions according to these markers. The cluster analysis revealed that these 36 accessions could be classified into five major clusters as B. juncea, B. napus and B. rapa, B. carinata and B. nigra, B. oleracea , Eruca sativa Mill at cutoff value 0.700. The results were consistent with the traditional classification. There are four haplotypes in B. juncea and only one in B. oleracea . The cytoplasmic diversity was higher in B. juncea than that in B. oleracea accessions.

对36份供试材料（15份甘蓝种材料、12份芥菜型油菜、4份不同细胞质类型的甘蓝型油菜、2份白菜型油菜及黑芥、埃塞俄比亚芥、芸芥各1份）进行叶绿体和线粒体SSR分析。7对多态性叶绿体特异的SSR引物在参试材料中共检测到31条多态性条带，每个位点等位基因为3~8个，平均4.43个，PIC值为0.234~0.711，平均为0.550。 2对线粒体特异的SSR引物检测到多态性条带数分别为3和5，PIC值分别为0.409和0.558。将线粒体与叶绿体的SSR标记合并，36份材料的遗传相似系数为0.538~1.000，在遗传相似系数 0.700 处，36份材料可明显分为5类，分别为芥菜型油菜、甘蓝型油菜和白菜型油菜、埃塞俄比亚芥和黑芥、甘蓝、芸芥，结果与传统的分类一致。芥菜型油菜内存在4种单倍型，而甘蓝仅有一种单倍型，芥菜型油菜材料间的细胞质遗传多样性高于甘蓝种材料间的细胞质多样性。