关键词: 细菌性果斑病, 西瓜种子, 酶联免疫吸附测定法, PCR检测法

Abstract:

Bacterial fruit blotch (Acidovorax citrulli) is a worldwide disease caused by Acidovorax avenae subsp. citrulli, which seriously endangers the crops of the gourd family. The disease is mainly transmitted through seeds at a long distance. If the seeds can be detected before planting, fruit blotch and other pathogens can effectively prevent economic losses to fruit farmers.In this experiment, enzyme-linked immunosorbent assay (ELISA) and PCR detection methods were used to detect bacterial fruit spot disease in 5 watermelon seeds, aiming to compare two different detection methods and explore their respective advantages and disadvantages. After comparing the two methods, it was found that the experimental results of ELISA detection method were easy to be affected by external factors, and the operation requirements were relatively high, and the accuracy was not as good as that of PCR detection method. However, in the PCR detection method, primer selection was very important, and the primer selection was directly related to the accuracy of the experiment. Therefore, the exploration of primer selection for PCR detection of bacterial fruit spot disease should be continued.

Key words: Acidovorax citrulli, watermelon seed, enzyme-linked immunosorbent assay, PCR detection