浙江农业科学

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (3): 702-705.DOI: 10.16178/j.issn.0528-9017.20240529

• 植保技术 • 上一篇    下一篇

海宁梨霉斑病病原鉴定

汤洁静1(), 郑放1, 阮赞誉2, 李红叶1,*()   

  1. 1.浙江大学 生物技术研究所,浙江 杭州 310058
    2.海宁市土肥植保能源技术服务站,浙江 海宁 314400
  • 收稿日期:2024-07-01 出版日期:2025-03-11 发布日期:2025-04-02
  • 通讯作者: 李红叶
  • 作者简介:李红叶,女,教授,博士,研究方向为植物病原真菌鉴定和致病机制研究,E-mail:hyli@zju.edu.cn。
    汤洁静,女,博士,研究方向为植物病原真菌鉴定和致病机制研究,E-mail:tangjiejing@zju.edu.cn。

Identification of the pear mold spot pathogen in Haining

TANG Jiejing1(), ZHENG Fang1, RUAN Zanyu2, LI Hongye1,*()   

  1. 1. Institute of Biotechnology,Zhejiang University, Hangzhou 310058, Zhejiang
    2. Haining Soil Fertilizer Plant Protection Energy Technology Service Station, Haining 314400, Zhejiang
  • Received:2024-07-01 Online:2025-03-11 Published:2025-04-02
  • Contact: LI Hongye

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摘要：

梨是我国第三大果树,也是浙江果树产业重要组成部分,近年来一种未知病因的斑点病在嘉兴海宁市的梨园流行,造成经济损失。本研究采用对角线取样法从浙江嘉兴海宁市黄湾镇钱江村农户果园采集5株梨树的发病叶片和果实,体视镜下观察子实体形态,同时对50片病叶和50个病果进行近似病菌分离和纯化。子实体形态学观察结果显示,病原菌为尾孢类真菌。进一步对13株菌株进行rDNA-ITS分子鉴定,结果显示,扩增所得序列与苹果假尾孢Pseudocercospora mali 菌株MUCC 886的ITS序列一致性达99.8%,与江西和广西壮族自治区报道的梨霉斑病病原一致。因此,我们认为海宁梨霉斑病的病原为苹果假尾孢。本研究首次描述了梨霉斑病在果实上的症状,研究结果为梨霉斑病的防治提供了依据。

关键词: 梨霉斑病, 病原菌, 鉴定, 苹果假尾孢

Abstract:

Pear is the third important fruit in China and constitutes an important component of the fruit industry in Zhejiang Province. In recent years, a spot disease of unknown etiology has been prevalent in pear orchards and caused economic losses in Haining, Jiaxing, Zhejiang. In this study, we collected diseased leaves and fruits from five pear trees in the orchards of Qianjiang Village, Huangwan, Haining, Jiaxing, using a diagonal sampling method. The morphology of the fruiting bodies was observed under a stereomicroscope, and the isolation and purification of supspected pathogen were conducted on 50 diseased leaves and 50 diseased pears. The results of morphology obsenation of fruiting bodies showed that the pathogen was a species of Cercosporioid. Furthermore, molecular identification of 13 isolates was performed with rDNA-ITS sequencing, and the results showed a 99.8% sequence identity with the ITS sequence of Pseudocercospora mali strain MUCC 886. Therefore, we proposed that the pathogen of pear mold spot in Haining was Pseudocercospora mali. In this study, the symptoms of pear mold spot on fruits was described at the first time, and the results provided a reference for the control of pear mold spot.

Key words: pear mold spot, pathogen, identification, Pseudocercospora mali

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