Study progress on the application and detection techniques of brassinolide plant growth regulators in fruit crops

doi:10.16178/j.issn.0528-9017.20250096

Abstract

Abstract:

Brassinolide compounds are excellent plant growth regulators characterized by promoting fruit crop growth and development, enhancing fruit quality, and being environmentally friendly. They have broad application prospects in the cultivation and production of fruit crops. As their application becomes more widespread, it is necessary to conduct safety assessments. However, due to their low residues in fruit crops and the complexity of fruit matrices, quantitative analysis poses significant challenges. Therefore, selecting reasonable and efficient detection techniques is of great significance. This article provided an overview of the application and detection techniques of brassinolide plant growth regulators in fruit crops, aiming to serve as a reference for future study.

Key words: brassinolide compound, fruit crop, detection technique, solid-phase extraction, chromatography

CLC Number:

S482.8

LIU Yang, YONG Yanhua, BIAN Haitao, SHI Zhen, YU Wei, LIU Ziyan, DONG Ruinan, YU Yang. Study progress on the application and detection techniques of brassinolide plant growth regulators in fruit crops[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(1): 86-90.

References 27

[1]	FRIDMAN Y, SAVALDI-GOLDSTEIN S. Brassinosteroids in growth control: how, when and where[J]. Plant Science, 2013, 209: 24-31.
[2]	吕习栋, 袁留斌. 油菜素内酯研究进展[J]. 农业灾害研究, 2013, 3(1): 56-57.
	LYU X D, YUAN L B. Research progress of brassinosteroids[J]. Journal of Agricultural Catastrophology, 2013, 3(1): 56-57.
[3]	曹立冬, 吴进龙, 李友顺, 等. 芸苔素内酯类化合物的合成与分析方法研究进展[J]. 农药科学与管理, 2016, 37(9): 12-19.
	CAO L D, WU J L, LI Y S, et al. The synthetic and analytical methods of brassinolide analogues[J]. Pesticide Science and Administration, 2016, 37(9): 12-19.
[4]	孙陈铭, 蔡岩, 苗志伟. 新型绿色植物生长调节剂: 芸苔素内酯的研究进展[J]. 化学教育(中英文), 2022(6): 1-8.
	SUN C M, CAI Y, MIAO Z W. Research progress of new type green plant growth regulator: brassinolide[J]. Chinese Journal of Chemical Education, 2022(6): 1-8.
[5]	BAJGUZ A, TRETYN A. The chemical characteristic and distribution of brassinosteroids in plants[J]. Phytochemistry, 2003, 62(7): 1027-1046.
[6]	李玉珠, 王艳玲, 吴丹, 等. 天然植物生长调节剂14-羟基芸苔素甾醇在猕猴桃授粉中的应用[J]. 北方果树, 2021(5): 5-8.
	LI Y Z, WANG Y L, WU D, et al. Application of natural plant growth regulator 14-hydroxylated brassinosteroid in kiwifruit artificial pollination[J]. Northern Fruits, 2021(5): 5-8.
[7]	刘静, 容新民. 芸苔素内酯对促成栽培夏黑葡萄果实品质的影响[J]. 安徽农业科学, 2015, 43(33): 58-59.
	LIU J, RONG X M. Effects of brassinolide on fruit quality of summer cultivation black grape[J]. Journal of Anhui Agricultural Sciences, 2015, 43(33): 58-59.
[8]	陆剑飞. 芸苔素内酯对4种水果经济性状和品质的影响[J]. 浙江农业科学, 2014, 55(7): 1032-1035.
	LU J F. Effects of brassinolide on economic traits and quality of four kinds of fruits[J]. Journal of Zhejiang Agricultural Sciences, 2014, 55(7): 1032-1035.
[9]	潘建春. 0.01%芸苔素内酯可溶液剂在葡萄上的应用效果研究[J]. 现代农业科技, 2015(8): 152-152.
	PAN J C. Study on the application effect of 0.01% brassinolide soluble liquid on grapes[J]. Modern Agricultural Science and Technology, 2015(8): 152-152.
[10]	李卫伟, 张武云, 曹挥, 等. 芸苔素内酯与吡唑醚菌酯混施对苹果生长发育的影响[J]. 山西农业科学, 2021, 49(1): 50-53.
	LI W W, ZHANG W Y, CAO H, et al. Effect of brassinolide combined with pyraclostrobin on growth and development of apple trees[J]. Journal of Shanxi Agricultural Sciences, 2021, 49(1): 50-53.
[11]	SHARMA P, JHA A B, DUBEY R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions[J]. Journal of Botany, 2012, 2012(1): 217037.
[12]	TANVEER M, SHAHZAD B, SHARMA A, et al. 24-epibrassinolide application in plants: an implication for improving drought stress tolerance in plants[J]. Plant Physiology and Biochemistry, 2019, 135: 295-303.
[13]	OGWENO J O, SONG X S, SHI K, et al. Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum[J]. Journal of Plant Growth Regulation, 2008, 27(1): 49-57.
[14]	束红梅, 郭书巧, 沈新莲, 等. 油菜素内酯对NaCl胁迫下棉花幼苗生理特性的影响[J]. 江苏农业学报, 2011, 27(6): 198-202.
	SHU H M, GUO S Q, SHEN X L, et al. Cotton physiology affected by brassinosteroid under NaCl stress[J]. Jiangsu Journal of Agricultural Sciences, 2011, 27(6): 198-202.
[15]	LEE K, JOHNSON V L, BLAKLEY B R. The effect of exposure to a commercial 2, 4-D formulation during gestation on the immune response in CD-1 mice[J]. Toxicology, 2001, 165(1): 39-49.
[16]	林舒婷, 张慧玲, 童萍, 等. 固相萃取-色谱/质谱技术在油菜素内酯分析中的研究进展[J]. 分析测试技术与仪器, 2021, 27(2): 90-99.
	LIN S T, ZHANG H L, TONG P, et al. Advances in analysis of brassinosteroids based on solid phase extraction-chromatography/mass spectrometry[J]. Analysis and Testing Technology and Instruments, 2021, 27(2): 90-99.
[17]	虞冰, 刘莉, 汪志威, 等. QuEChERS-超高效液相色谱-串联质谱法快速测定草莓中2, 4-表芸苔素内酯残留量[J]. 浙江农业科学, 2017, 58(9): 1538-1540, 1545.
	YU B, LIU L, WANG Z W, et al. Rapid determination of 2, 4-epibrassinolide residues in strawberries by QuEChERS-UPLC-MS/MS[J]. Journal of Zhejiang Agricultural Sciences, 2017, 58(9): 1538-1540, 1545.
[18]	诸力, 陈红平, 柴云峰, 等. 分散固相萃取-超高效液相色谱-串联质谱法测定不同茶类中2, 4-表芸苔素内酯残留[J]. 茶叶科学, 2018, 38(6): 589-594.
	ZHU L, CHEN H P, CHAI Y F, et al. Determination of 2, 4-epibrassinolide in different teas by ultra performance liquid chromatography coupled to tandem mass spectrometry with dispersive solid phase extraction[J]. Journal of Tea Science, 2018, 38(6): 589-594.
[19]	杜功名, 查欣欣, 吴甜甜, 等. QuPPe-超高效液相色谱-串联质谱法测定葡萄中28-表高芸苔素内酯残留量[J]. 现代农药, 2021, 20(6): 44-46.
	DU G M, ZHA X X, WU T T, et al. Simultaneous determination of residue of 28-homobrassinolide in grapes by QuPPe-UPLC-MS/MS[J]. Modern Agrochemicals, 2021, 20(6): 44-46.
[20]	WANG X, MA Q, LI M, et al. Automated and sensitive analysis of 28-epihomobrassinolide in Arabidopsis thaliana by on-line polymer monolith microextraction coupled to liquid chromatography-mass spectrometry[J]. Journal of Chromatography A, 2013, 1317: 121-128.
[21]	WANG X Y, XIONG C F, YE T T, et al. Online polymer monolith microextraction with in situ derivatization for sensitive detection of endogenous brassinosteroids by LC-MS[J]. Microchemical Journal, 2020, 158: 105061.
[22]	XIN P Y, LI B B, YAN J J, et al. Pursuing extreme sensitivity for determination of endogenous brassinosteroids through direct fishing from plant matrices and eliminating most interferences with boronate affinity magnetic nanoparticles[J]. Analytical and Bioanalytical Chemistry, 2018, 410(4): 1363-1374.
[23]	孙太凡, 叶非. 酶免疫分析技术在农药残留分析中的应用[J]. 理化检验:化学分册, 2005, 41(3): 223-226.
	SUN T F, YE F. Application of enzyme immunoassay technology in pesticide residue analysis[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2005, 41(3): 223-226.
[24]	BHARDWAJ R, KAUR S, NAGAR P K, et al. Isolation and characterization of brassinosteroids from immature seeds of Camellia sinensis (O) Kuntze[J]. Plant Growth Regulation, 2007, 53(1): 1-5.
[25]	LIU X G, DONG F S, HU H, et al. Residue analysis of propionylbrassinolide in fruit and vegetables by GC-MS[J]. Chromatographia, 2009, 69(11): 1453-1456.
[26]	何钰, 廖朝选, 龚会琴, 等. HPLC-MS/MS法测定水体中植物激素28-高芸苔素内酯的含量[J]. 农药, 2022, 61(3): 195-197, 202.
	HE Y, LIAO C X, GONG H Q, et al. Determination of the content of phytohormone 28-homobrassinolide in water by HPLC-MS/MS[J]. Agrochemicals, 2022, 61(3): 195-197, 202.
[27]	王菲迪, 王莉, 吴声敢, 等. UPLC-MS/MS测定14-羟基芸苔素甾醇[J]. 浙江农业科学, 2020, 61(3): 544-545, 548.
	WANG F D, WANG L, WU S M, et al. Determination of 14-hydroxylated brassinosteroid by UPLC-MS/MS[J]. Journal of Zhejiang Agricultural Sciences, 2020, 61(3): 544-545, 548.