doi:10.16178/j.issn.0528-9017.20213218

Abstract

CLC Number:

S41-30

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References 49

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检测方法	材料要求	适用场景	特异性	检测速度	工作量	检测成本
田间症状观察	活体植株	田间或实验室	低	慢	大	低
生物学测定	化学药剂	田间或实验室	中	慢	大	中
免疫学检测	酶标仪	田间或实验室	高	快	中	中
分子检测	PCR仪	实验室	高	快	中	中

检测方法	材料要求	适用场景	特异性	检测速度	工作量	检测成本
田间症状观察	活体植株	田间或实验室	低	慢	大	低
生物学测定	化学药剂	田间或实验室	中	慢	大	中
免疫学检测	酶标仪	田间或实验室	高	快	中	中
分子检测	PCR仪	实验室	高	快	中	中

检测技术	引物名称	序列方向	引物序列（5'-3'）	产物大小/bp	参考文献
KN-PCR	KNF	Forward	CACGATACATGGGCTTATGC	492	[11]
	KNR	Reverse	CTTTTCATCCACACACTCCG
RG-PCR	PsaF1	Forward	TTTTGCTTTGCACACCCGATTTT	280	[34]
	PsaR2	Reverse	CACGCACCCTTCAATCAGGATG	175
	PsaF3	Forward	ACCTGGTGAAGTTGGTCAGAGC
	PsaR4	Reverse	CGCACCCTTCAATCAGGATG
双重PCR	KNF	Forward	CACGATACATGGGCTTATGC	492	[36]
	KNR	Reverse	CTTTTCATCCACACACTCCG	226
	AvrDdpx-F	Forward	TTTCGGTGGTAACGTTGGCA
	AvrDdpx-R	Reverse	TTCCGCTAGGTGAAAAATGGG
巢氏PCR	cf1-1	Forward	GGCGCTCCCTCGCACTT	665	[32]
	cf1-2	Reverse	GGTATTGGCGGGGGTGC	310
	cf1-3	Forward	TCCTACGGTACGACGGAGTC
	cf1-4	Reverse	ACGGGGGATATGGAATAAGC
巢氏PCR	B1	Forward	GTGATTATCGGCACTGACGG	631	[37]
	B2	Reverse	CAAGCACACCATTGGTCATTGA	502
	KNF	Forward	CACGATACATGGGCTTATGC
	KNR	Reverse	CTTTTCATCCACACACTCCG
PCR	F7	Forward	CAATCATTTCGCCAGACGC	950	[38]
	R7	Reverse	CTACGAGGTTAGGTTCAGAGT
PCR-C	P0F	Forward	CTGCAACAGGCGACGGCGAGGC	243	[41]
	P6R	Reverse	CATAGGCTTCTGGTTTTCTTCCTGATCC
Real-time PCR-E	P3F	Forward	GGTTTCGGACACCGCAGGTTCTACCGAG	147
	P5R1	Reverse	CTTCCTGATCCCCGTTACCCATCGAC
Real-time PCR-F	P3F	Forward	GGTTTCGGACACCGCAGGTTCTACCGAG	158
	P6R	Reverse	CATAGGCTTCTGGTTTTCTTCCTGATCC
PSA LAMP	F3	Forward	GGCTCTCCTAGCAAGCATAC	无需电泳	[42]
	B3	Reverse	TGAGAAGGGACGCAACCA
	FIP	Forward	CGGATTCGCAACGCTCCAAGATCTGCTGAGCACGTTGGTC
	BIP	Reverse	ACTCTTCCGCAACGAGTTTGGGTCCCCACATGGAGTTGTCT
	loopF	Forward	TGCCGATCGAGTATCCATTTCCT
	loopR	Reverse	AGCCTTTTCCGAACGGTCT
PSA3 LAMP	F3	Forward	GCTATGGAATCCATTGCGGT
	B3	Reverse	CGCATCTGCTGGATCATCC
	FIP	Forward	ATCCCTTGCCCAGCACGAACATGAGGTCGAGGTGTCTGA
	BIP	Reverse	ATCCACAGTGGGTACACGGACGGGGGCACCTTCTTTCTTGG
	loopF	Forward	GGCCTTTTCAATGCGGTCAATATCC
	loopR	Reverse	GGTGTCAATCTGGTGGTGCAT
Psa5（Biovar 5）-PCR	Con002F	Forward	AACTCATACCCTGCGGTCAC	449	[27]
	Con002R	Reverse	GACACCGAGCAAAACCAAAT
	Con034F	Forward	CCAAACAACGTCTGGGCTAT	450
	Con034R	Reverse	TCGGCCTAGCTACGAGTGAT
	Con044F	Forward	AAGCGCCTTAATCTCGTTCA	470
	Con044R	Reverse	ATTCCGGATTGGGTATCACA
	Con067F	Forward	ATTTTAACGCCCATCTGCAC	439
	Con067R	Reverse	CTGCGGATTGCAACAGTCTA

检测技术	引物名称	序列方向	引物序列（5'-3'）	产物大小/bp	参考文献
KN-PCR	KNF	Forward	CACGATACATGGGCTTATGC	492	[11]
	KNR	Reverse	CTTTTCATCCACACACTCCG
RG-PCR	PsaF1	Forward	TTTTGCTTTGCACACCCGATTTT	280	[34]
	PsaR2	Reverse	CACGCACCCTTCAATCAGGATG	175
	PsaF3	Forward	ACCTGGTGAAGTTGGTCAGAGC
	PsaR4	Reverse	CGCACCCTTCAATCAGGATG
双重PCR	KNF	Forward	CACGATACATGGGCTTATGC	492	[36]
	KNR	Reverse	CTTTTCATCCACACACTCCG	226
	AvrDdpx-F	Forward	TTTCGGTGGTAACGTTGGCA
	AvrDdpx-R	Reverse	TTCCGCTAGGTGAAAAATGGG
巢氏PCR	cf1-1	Forward	GGCGCTCCCTCGCACTT	665	[32]
	cf1-2	Reverse	GGTATTGGCGGGGGTGC	310
	cf1-3	Forward	TCCTACGGTACGACGGAGTC
	cf1-4	Reverse	ACGGGGGATATGGAATAAGC
巢氏PCR	B1	Forward	GTGATTATCGGCACTGACGG	631	[37]
	B2	Reverse	CAAGCACACCATTGGTCATTGA	502
	KNF	Forward	CACGATACATGGGCTTATGC
	KNR	Reverse	CTTTTCATCCACACACTCCG
PCR	F7	Forward	CAATCATTTCGCCAGACGC	950	[38]
	R7	Reverse	CTACGAGGTTAGGTTCAGAGT
PCR-C	P0F	Forward	CTGCAACAGGCGACGGCGAGGC	243	[41]
	P6R	Reverse	CATAGGCTTCTGGTTTTCTTCCTGATCC
Real-time PCR-E	P3F	Forward	GGTTTCGGACACCGCAGGTTCTACCGAG	147
	P5R1	Reverse	CTTCCTGATCCCCGTTACCCATCGAC
Real-time PCR-F	P3F	Forward	GGTTTCGGACACCGCAGGTTCTACCGAG	158
	P6R	Reverse	CATAGGCTTCTGGTTTTCTTCCTGATCC
PSA LAMP	F3	Forward	GGCTCTCCTAGCAAGCATAC	无需电泳	[42]
	B3	Reverse	TGAGAAGGGACGCAACCA
	FIP	Forward	CGGATTCGCAACGCTCCAAGATCTGCTGAGCACGTTGGTC
	BIP	Reverse	ACTCTTCCGCAACGAGTTTGGGTCCCCACATGGAGTTGTCT
	loopF	Forward	TGCCGATCGAGTATCCATTTCCT
	loopR	Reverse	AGCCTTTTCCGAACGGTCT
PSA3 LAMP	F3	Forward	GCTATGGAATCCATTGCGGT
	B3	Reverse	CGCATCTGCTGGATCATCC
	FIP	Forward	ATCCCTTGCCCAGCACGAACATGAGGTCGAGGTGTCTGA
	BIP	Reverse	ATCCACAGTGGGTACACGGACGGGGGCACCTTCTTTCTTGG
	loopF	Forward	GGCCTTTTCAATGCGGTCAATATCC
	loopR	Reverse	GGTGTCAATCTGGTGGTGCAT
Psa5（Biovar 5）-PCR	Con002F	Forward	AACTCATACCCTGCGGTCAC	449	[27]
	Con002R	Reverse	GACACCGAGCAAAACCAAAT
	Con034F	Forward	CCAAACAACGTCTGGGCTAT	450
	Con034R	Reverse	TCGGCCTAGCTACGAGTGAT
	Con044F	Forward	AAGCGCCTTAATCTCGTTCA	470
	Con044R	Reverse	ATTCCGGATTGGGTATCACA
	Con067F	Forward	ATTTTAACGCCCATCTGCAC	439
	Con067R	Reverse	CTGCGGATTGCAACAGTCTA

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