不同来源生物支架在细胞培养肉中的应用

doi:10.16178/j.issn.0528-9017.20250383

摘要/Abstract

摘要：

为了获得口感和营养更接近肉的细胞培养肉，来自不同动物、植物、微生物的生物支架多被用于支持成肌细胞的生长，促进细胞培养肉技术的发展和成熟。这些不同来源的生物支架在孔隙率、黏附性、生物相容性等方面存在差异，而特性上的差异也正是影响支架与细胞相互作用的重要因素以及衡量支架品质的指标。本研究以不同生物支架在细胞培养肉中的表现特性为出发点，探究不同生物支架发挥作用的内在机制以及成肌细胞对生物支架的选择结合机制，旨在为细胞培养肉后续研究中生物支架的选择提供有效参考和判断依据。

关键词: 细胞培养肉, 生物支架, 孔隙率, 黏附性, 生物相容性

Abstract:

To obtain cell-cultured meat which is closer to the taste and nutrition of real meat，biological scaffolds derived from various animals，plants，and microorganisms are employed to support the growth of myoblasts，thereby promoting the development and maturation of cell-cultured meat technology. These biological scaffolds from different sources have differences in porosity，adhesion，biocompatibility，etc.，and the differences in characteristics are precisely the important factors affecting the interaction between scaffolds and cells as well as the indicators for measuring the quality of scaffolds. This study took the characteristics exhibited by different biological scaffolds in cell-cultured meat as the starting point to explore the internal mechanisms of the functions of different biological scaffolds and the selection and binding mechanism of biological scaffolds by myoblasts，aiming to provide effective references and judgment basis for the selection of biological scaffolds in the subsequent research of cell-cultured meat.

Key words: cell-cultured meat, biological scaffold, porosity, adhesion, biocompatibility

中图分类号:

TS219

段羽佳, 金爱林, 贾梦, 孙晋跃, 周文文. 不同来源生物支架在细胞培养肉中的应用[J]. 浙江农业科学, 2026, 67(7): 1789-1795.

DUAN Yujia, JIN Ailin, JIA Meng, SUN Jinyue, ZHOU Wenwen. Application of biological scaffolds from different sources in cell-cultured meat[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(7): 1789-1795.

图/表 3

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细胞种类	支架材料	增量表达的蛋白	参考文献
牛肌肉卫星细胞（bMuSC）	蛋壳膜	增殖标志物与分化标志物	［11］
猪骨骼肌卫星细胞（PSC）、猪脂肪组织来源干细胞（ADSC）	高粱醇溶蛋白	脂肪酸转运蛋白4（FABP4）	［31］
小鼠成肌细胞（C2C12）、猪骨骼肌卫星细胞（PSC）	谷物醇溶蛋白	整合素β1、N-钙黏蛋白	［33］
小鼠成纤维细胞（NIH3T3）	大豆膳食纤维-蛋白质双交联水凝胶	F-肌动蛋白	［45］
猪原肠胚前上胚层干细胞（pgEpiSCs-MC）	魔芋葡甘露聚糖-海藻酸钠-钙离子	肌纤维分化相关蛋白	［46］
牛成肌细胞	甲壳素、海藻酸钠、槲皮素	胶原蛋白Ⅰ、胶原蛋白Ⅲ、弹性蛋白、纤连蛋白	［47］
鱼卫星细胞（PSC）	明胶水凝胶与5种植物来源的葡萄糖基纳米颗粒（GNP）	整合素ITG-A1	［48］

细胞种类	支架材料	增量表达的蛋白	参考文献
牛肌肉卫星细胞（bMuSC）	蛋壳膜	增殖标志物与分化标志物	［11］
猪骨骼肌卫星细胞（PSC）、猪脂肪组织来源干细胞（ADSC）	高粱醇溶蛋白	脂肪酸转运蛋白4（FABP4）	［31］
小鼠成肌细胞（C2C12）、猪骨骼肌卫星细胞（PSC）	谷物醇溶蛋白	整合素β1、N-钙黏蛋白	［33］
小鼠成纤维细胞（NIH3T3）	大豆膳食纤维-蛋白质双交联水凝胶	F-肌动蛋白	［45］
猪原肠胚前上胚层干细胞（pgEpiSCs-MC）	魔芋葡甘露聚糖-海藻酸钠-钙离子	肌纤维分化相关蛋白	［46］
牛成肌细胞	甲壳素、海藻酸钠、槲皮素	胶原蛋白Ⅰ、胶原蛋白Ⅲ、弹性蛋白、纤连蛋白	［47］
鱼卫星细胞（PSC）	明胶水凝胶与5种植物来源的葡萄糖基纳米颗粒（GNP）	整合素ITG-A1	［48］