海洋柴油降解细菌的筛选鉴定及其生物表面活性剂特性

doi:10.16178/j.issn.0528-9017.20180312

浙江农业科学 ›› 2018, Vol. 59 ›› Issue (3): 400-404.DOI: 10.16178/j.issn.0528-9017.20180312

海洋柴油降解细菌的筛选鉴定及其生物表面活性剂特性

马斌斌^1,2,3, 马恒轶^1,2, 李少君^1,2, 邓欢欢^1,2*

1.温州医科大学公共卫生与管理学院,浙江温州 325035;
2.浙江省流域水环境与健康风险研究重点实验室,浙江温州 325035;
3.温州伟明环保能源有限公司,浙江温州 325000

收稿日期:2017-12-28 出版日期:2018-03-11
通讯作者: 邓欢欢(1979—),男,湖北荆门人,副教授,博士,研究方向为环境工程,E-mail:now57@163.com。
作者简介:马斌斌(1987—),男,甘肃武山人,硕士,研究方向为环境微生物,E-mail:binmbin@163.com。
基金资助:
国家自然科学基金(51108351,51108350); 浙江省自然科学基金(LY14D060009,LY17E090006); 温州市科技局项目(S20140034,S20140036); 温州医科大学校级教改课题(YBKG201432,YB2012044)

Received:2017-12-28 Online:2018-03-11

摘要/Abstract

摘要： 从浙江舟山渔场油污染严重的海水中经富集培养、测定柴油降解率,初筛到7株柴油降解菌。对初筛到的7株菌通过免疫溶血实验、测定发酵液的表面张力和排油圈直径作进一步复筛,最终筛选出1株产生物表面活性剂的高效柴油降解细菌,经生理生化实验、16s rRNA鉴定为不动杆菌(Acinetobacter)。该菌株对柴油的降解率可达82.7%,发酵液液体表面张力可从74 mN·m^-1降至21.66 mN·m^-1,产生的生物表面活性剂经薄层色谱、傅里叶变换红外光谱、气相色谱质谱联用鉴定为含有C₁₅脂肪酸的脂肽,临界胶束浓度为52.2 mg·L^-1,乳化性能为64.7%。

中图分类号:

X55
Q939.9

马斌斌, 马恒轶, 李少君, 邓欢欢. 海洋柴油降解细菌的筛选鉴定及其生物表面活性剂特性[J]. 浙江农业科学, 2018, 59(3): 400-404.

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海洋柴油降解细菌的筛选鉴定及其生物表面活性剂特性

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