An efficient tissue culture technique using strawberry peduncles as explants

doi:10.16178/j.issn.0528-9017.20231141

Abstract

Abstract:

Healthy seedling is important to strawberry industry, while tissue culture is an important role in producing it. This study explored a rapid propagation technology using strawberry peduncles as explants by comparing the rate of adventitious shoot and the number of regenerated shoots from different organs and cultivated varieties. It was found that peduncles with flower buds and bracts can form adventitious shoots and clustered seedlings in 3 weeks and 6 weeks respectively, and the adventitious shoot regeneration rate can reach 100%. It provides a strong guarantee for the preservation of strawberry germplasm resources and the production of healthy seedlings.

Key words: strawberry, peduncle, bract, adventitious shoot, stolon, tissue culture

CLC Number:

S668.4

CAI Xin, JIANG Jingyong, CHEN Yinlong, WANG Jiaoyang, MI Min, QIU Liping. An efficient tissue culture technique using strawberry peduncles as explants[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(7): 1614-1617.

Figures/Tables 5

Fig.1 The process of tissue culture and seedling formation using strawberry peduncles as explants(Benihoppe)

Fig.2 Different phenotypes produced by tissue culture using different strawberry organs of Fenyu as explants

Table 1 Comparison of adventitious shoots using different organs of stawberry as explants

组别	不定芽再生率/%	每外植体再生芽数
A	100.0 (26/26)	8.74±0.76 a (n=23)
B	36.0 (9/25)	4.25±0.86 b (n=8)
C	72.7 (8/11)	5.88±1.56 b (n=8)
D	100.0 (21/21)	4.57±0.65 b (n=21)

Fig.3 Phenotype of tissue culture of different strawberry cultivars using peduncle as explant

Table 2 Comparison of adventitious bud regeneration in different strawberry cultivars

品种	不定芽再生率/%	每外植体再生芽数
粉玉	100(26/26)	8.74±0.76 b(n=23)
越秀	100(15/15)	10.54±0.98 ab(n=13)
越心	100(30/30)	9.26±0.83 b(n=23)
章姬	100(20/20)	10.85±1.41 ab(n=20)
红颊	100(26/26)	13.74±1.43 a(n=19)

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