Abstract:

In order to investigate the community composition of rhizosphere microorganisms and endophytes in okra, we used the spread plate method to isolate and purify the microorganisms in the rhizosphere soil, root tissue, and stem tissue of okra. The isolated strains were identified through morphological characteristics observation, physiological and biochemical tests combined with molecular biological identification. The results showed that the microbial species from rhizosphere soil of okra were abundant. A total of 18 species were identified, and the number of bacterial were the highest, followed by fungi, and the number of actinomycetes was the lowest. A total of 9 species were isolated and identified from root tissue and stem tissue of okra, and these microorganisms were common shared between okra tissue and rhizosphere soil, which indicated the species homology between the endophytes in the root tissue and stem tissue of okra and the microorganisms in the rhizosphere soil. Several isolated strains including Bacillus subtilis, Pseudomonas monteilii and Rhizobium pusense are able to fix nitrogen, dissolve phosphorus, and produce auxin that can benefit plant growth. Our study provides basis for deep study of distribution of microorganisms and their roles in the growth and development of okra.

Key words: okra, rhizosphere microorganism, endophyte, isolation and identification