Effect of different organic materials combined with nitrification inhibitor on soil nitrogen conversion in paddy field under dry-wet alternation

doi:10.16178/j.issn.0528-9017.20240563

Abstract

Abstract:

Microcosmic culture experiments were used to investigate the effects of combined application of different organic materials, nitrification inhibitor and chemical nitrogen fertilizers on soil nitrogen transformation in paddy field under the condition of dry-wet alternation. It was found that under dry-wet alternation conditions, 3,4-dimethylpyrazole phosphate(DMPP) can increase soil ammonium nitrogen content, inhibit the abundance of ammonia-oxidizing bacteria, and reduce the soil net nitrification rate. Furthermore, the application of DMPP with organic fertilizer was more effective in increasing soil pH value, and content of dissolved organic carbon and DOC ammonium nitrogen content.

Key words: paddy soil, organic materials, nitrification inhibitor, dry-wet alternation, nitrogen conversion

CLC Number:

S146

SHENG Yanfei, ZHANG Wenhan, ZHANG Yuanyuan, WANG Feng, FU Liqing, HUANG Qiying, LU Zhongcheng, YE Nan, LU Jiujin. Effect of different organic materials combined with nitrification inhibitor on soil nitrogen conversion in paddy field under dry-wet alternation[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2324-2328.

Figures/Tables 7

References 20

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引物名称	引物序列(5'—3')	片段长度/bp
AOA-amoAF	STAATGGTCTGGCTTAGACG	635
AOA-amoAR	GCGGCCATCCATCTGGTATGT
AOB-amoAF	GGGGTTTCTACTGGTGGT	491
AOB-amoAR	CCCCTCKGSAAAGCCTTCTTC

引物名称	引物序列(5'—3')	片段长度/bp
AOA-amoAF	STAATGGTCTGGCTTAGACG	635
AOA-amoAR	GCGGCCATCCATCTGGTATGT
AOB-amoAF	GGGGTTTCTACTGGTGGT	491
AOB-amoAR	CCCCTCKGSAAAGCCTTCTTC

处理	不同处理时间(d)的土壤净硝化速率/ (mg·kg^-1·d^-1)
处理	7	14	21	28
CK	0.19 b	0.57 b	2.54 b	3.29 a
U	0.24 b	1.23 a	3.12 a	2.82 b
DMPP	0.09 c	-0.03 c	-0.15 d	0.31 d
BC	0.08 c	0.09 c	-0.28 d	0.79 c
S	0.17 b	-0.21 c	0.01 c	-0.01 e
M	1.44 a	-0.99 d	0.20 c	0.02 e

处理	不同处理时间(d)的土壤净硝化速率/ (mg·kg^-1·d^-1)
处理	7	14	21	28
CK	0.19 b	0.57 b	2.54 b	3.29 a
U	0.24 b	1.23 a	3.12 a	2.82 b
DMPP	0.09 c	-0.03 c	-0.15 d	0.31 d
BC	0.08 c	0.09 c	-0.28 d	0.79 c
S	0.17 b	-0.21 c	0.01 c	-0.01 e
M	1.44 a	-0.99 d	0.20 c	0.02 e

因子	DOC含量	pH值	AOB丰度	AOA丰度	$\text{NH} ^{+}_{4}$-N含量	$\text{NO} ^{-}_{3}$-N含量
pH值	0.886^**
AOB丰度	0.044	-0.032
AOA丰度	-0.380	-0.376	-0.311
$\text{NH} ^{+}_{4}$-N含量	0.335	0.572^**	-0.063	0.179
$\text{NO} ^{-}_{3}$-N含量	-0.234	-0.574^**	0.568^**	-0.061	-0.467^*
净硝化速率	-0.148	-0.500^*	0.627^**	-0.104	-0.502^*	0.972^**