Abstract:

In this study, five kinds of mycorrhizal fungi, including Glomus mossesa (Gm), Glomus hoi (Gh), Glomus versiforme (Gv), Glomus etunicatum (Ge) and Glomus aggregatum (Ga), were selected as the test materials, and the domesticated seedlings of Dendrobium officinale Kimura et Migo were tested under the conditions of 28 ℃ light for 14 h and 22 ℃ darkness for 10 h, to explore the effects of mycorrhizal fungi on the growth of Dendrobium officinale Kimura et Migo. The results showed that all of the five mycorrhizal fungi could infect Dendrobium officinale Kimura et Migo and form a symbiotic structure. Comprehensive analysis of the phenotype, fresh weight, nitrogen and phosphorus content, and polysaccharide content of Dendrobium officinale Kimura et Migo inoculated with five mycorrhizal fungi showed that the inoculated five mycorrhizal fungi could promote the growth of Dendrobium officinale Kimura et Migo, increase the content of nitrogen and phosphorus, and promote the increase of polysaccharide content. Among them, Gm has the best promoting effect on the growth, accumulation of nutrient elements and polysaccharide content of Dendrobium officinale Kimura et Migo, and is a suitable mycorrhizal fungi for the mycorrhizal production of Dendrobium officinale Kimura et Migo. By inoculating three different lines of Dendrobium officinale Kimura et Migo (red rod, rusty rod and green rod) with Gm, it was found that Gm also promoted the growth and polysaccharide accumulation of different lines. This study provided the advantageous material and theoretical basis for the mycorrhizal cultivation of Dendrobium officinale Kimura et Migo, and laid a foundation for the application of the mycorrhizal cultivation of Dendrobium officinale Kimura et Migo.

Key words: mycorrhizal symbiosis, Dendrobium officinale Kimura et Migo, growth effect, polysaccharide content