The influencing factors of stem tip culture and bulb propagat of lily Robina

doi:10.16178/j.issn.0528-9017.20240681

Abstract

Abstract:

In order to explore the influencing factors on stem tip culture and bulb propagation of lily, the tissue culture seedlings of lily Robina were selected as experimental materials, and the study was conducted through stem tip culture and culture of bulbs in bottles. The results showed that the size of the stem tip is a key factor affecting the survival rate of the stem tip. The survival rate of the 1.0 mm stem tip was about 5 percentage points higher than that of the 0.5 mm stem tip. Increasing the content of plant growth regulators and banana powder can improve the survival rate of the stem tip. Light culture (12 h·d^-1) was more conducive to bulb proliferation and growth than dark culture, and there was almost no obvious difference in the effect of 20 ℃ and 25 ℃ on bulb growth. Compared with dark culture, the number, diameter, fresh weight, and dry weight of bulbs obtained under light culture at 25 ℃ increased by 36.36%,15.33%,59.91% and 44.74%, respectively. The stem tip culture of lily Robina can be carried out by inoculating the stem tips with 1/2 MS+0.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA+5.0 g·L^-1 banana powder medium and directly culturing under light, and the cultivation conditions for bulbs can be selected as 25 ℃ and light cultivation.

Key words: lily, stem tip, bulb

CLC Number:

S682.2

WANG Yiting, LEI Xueming, LYU Yongping, MOU Haojie, LI Haiying, CHEN Zhi. The influencing factors of stem tip culture and bulb propagat of lily Robina[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(5): 1201-1205.

Figures/Tables 3

References 27

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温度/℃	培养条件	数量	直径/mm	总鲜重/g	总干重/g	总干物质含量/%
20	光培养	13±1.50 a b	10.56±0.38 a	6.34±0.39 b	1.02±0.05 a	16.24±0.41 b
	暗培养	12±1.26 b	9.34±0.49 b	4.26±0.53 c	0.77±0.07 b	18.09±0.49 a
25	光培养	15±1.57 a	10.61±0.12 a	7.26±0.17 a	1.10±0.03 a	15.30±0.35 c
	暗培养	11±0.51 b	9.20±0.33 b	4.54±0.51 c	0.76±0.07 b	16.80±0.38 b

温度/℃	培养条件	数量	直径/mm	总鲜重/g	总干重/g	总干物质含量/%
20	光培养	13±1.50 a b	10.56±0.38 a	6.34±0.39 b	1.02±0.05 a	16.24±0.41 b
	暗培养	12±1.26 b	9.34±0.49 b	4.26±0.53 c	0.77±0.07 b	18.09±0.49 a
25	光培养	15±1.57 a	10.61±0.12 a	7.26±0.17 a	1.10±0.03 a	15.30±0.35 c
	暗培养	11±0.51 b	9.20±0.33 b	4.54±0.51 c	0.76±0.07 b	16.80±0.38 b