Test and research on micro bud grafting technology of Ougan (Citrus suavissima Hort. ex Tanaka)

doi:10.16178/j.issn.0528-9017.20241004

Abstract

Abstract:

The experiment primarily focused on obtaining sterile rootstocks and scions for citrus micro-grafting, and investigated the effects of shoot tip size and grafting methods on the survival rate of in vitro micro-grafting in Ougan citrus. Additionally, the effectiveness of Huanglongbing (HLB) pathogen elimination in grafted seedlings was detected using RT-PCR technology. The results showed that local Zhuluan seeds and Guangxi trifoliate orange seeds exhibited the highest germination rate in the current year. Using Guangxi trifoliate orange seeds as rootstocks, the optimal germination conditions were achieved by disinfecting the seeds with 50% Bijieshi disinfectant for 10-20 minutes and culturing them in a water + agar medium, yielding a sterile germination rate of 43.5%. The survival rates of micro-grafting varied significantly among different rootstock varieties. Among the four rootstock varieties tested, the ease of micro-grafting survival followed the order: Zhuluan>trifoliate orange>fragrant orange>red tangerine. Triangular bud grafting was performed using a stem tip diameter of 0.2-0.4 mm. The survival rate of the stem tip was 42.5% after 30 days grafting. The removal rate of Huanglongbing was 41.6%.

Key words: Ougan (Citrus suavissima Hort. ex Tanaka), micro bud grafting, germination rate, survival rate

CLC Number:

S666.1

WANG Hong, HONG Fanglei, ZHENG Bosheng, YU Hongao. Test and research on micro bud grafting technology of Ougan (Citrus suavissima Hort. ex Tanaka)[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(4): 945-951.

Figures/Tables 13

References 12

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处理方式	砧木	培养基	当年萌芽率/%	平均萌芽率/%
1	枳壳	1	10.0±2.00 a	9.0±3.12 b
		2	6.3±1.53 b
		3	10.7±4.04 a
	朱栾	1	36.7±6.51 a	26.6±9.19 a
		2	25.0±5.00 b
		3	18.0±2.00 c
	香橙	1	11.0±1.00 a	10.8±2.28 b
		2	9.7±1.53 b
		3	11.7±3.79 a
	红橘	1	9.3±0.58 a	8.0±2.74 b
		2	5.0±2.65 b
		3	9.7±1.53 a
	平均值			13.6±9.12 b
2	枳壳	1	17.7±2.52 b	15.8±5.02 b
		2	9.7±1.53 c
		3	20.0±2.00 a
	朱栾	1	71.3±4.04 a	57.3±15.62 a
		2	62.3±6.03 b
		3	38.3±7.09 c
	香橙	1	20.0±2.00 a	17.4±4.64 b
		2	12.0±3.00 b
		3	20.3±2.52 a
	红橘	1	20.7±3.79 a	16.1±6.33 b
		2	8.3±1.53 b
		3	19.3±2.52 a
	平均值			26.7±19.96 a
3	枳壳	1	7.7±2.08 b	8.2±4.06 c
		2	4.3±1.53 c
		3	12.7±2.52 a
	朱栾	1	83.7±4.16 a	66.8±19.85 a
		2	75.7±3.06 ab
		3	41.0±2.65 c
	香橙	1	20.0±2.65 a	18.0±3.32 b
		2	19.0±3.61 a
		3	15.0±1.73 b
	红橘	1	21.7±2.89 a	17.8±3.80 b
		2	14.3±2.08 bc
		3	17.3±2.08 b
	平均值			27.7±25.29 a

处理方式	砧木	培养基	当年萌芽率/%	平均萌芽率/%
1	枳壳	1	10.0±2.00 a	9.0±3.12 b
		2	6.3±1.53 b
		3	10.7±4.04 a
	朱栾	1	36.7±6.51 a	26.6±9.19 a
		2	25.0±5.00 b
		3	18.0±2.00 c
	香橙	1	11.0±1.00 a	10.8±2.28 b
		2	9.7±1.53 b
		3	11.7±3.79 a
	红橘	1	9.3±0.58 a	8.0±2.74 b
		2	5.0±2.65 b
		3	9.7±1.53 a
	平均值			13.6±9.12 b
2	枳壳	1	17.7±2.52 b	15.8±5.02 b
		2	9.7±1.53 c
		3	20.0±2.00 a
	朱栾	1	71.3±4.04 a	57.3±15.62 a
		2	62.3±6.03 b
		3	38.3±7.09 c
	香橙	1	20.0±2.00 a	17.4±4.64 b
		2	12.0±3.00 b
		3	20.3±2.52 a
	红橘	1	20.7±3.79 a	16.1±6.33 b
		2	8.3±1.53 b
		3	19.3±2.52 a
	平均值			26.7±19.96 a
3	枳壳	1	7.7±2.08 b	8.2±4.06 c
		2	4.3±1.53 c
		3	12.7±2.52 a
	朱栾	1	83.7±4.16 a	66.8±19.85 a
		2	75.7±3.06 ab
		3	41.0±2.65 c
	香橙	1	20.0±2.65 a	18.0±3.32 b
		2	19.0±3.61 a
		3	15.0±1.73 b
	红橘	1	21.7±2.89 a	17.8±3.80 b
		2	14.3±2.08 bc
		3	17.3±2.08 b
	平均值			27.7±25.29 a

地区	当年萌芽率/%	污染率/%	第2年萌芽率/%	第2年污染率/%
山东	14.9±1.8 c	63.8±3.3 a	31.7±2.4 d	54.8±2.9
河南	12.4±2.2 c	61.3±4.5 a	33.4±2.0 cd	57.0±1.6
江苏	20.2±1.8 b	65.4±1.9 a	36.9±1.0 bc	55.0±1.6
浙江	23.8±2.8 b	62.8±2.1 a	40.4±1.5 b	49.2±0.7
广西	43.5±1.9 a	40.0±4.9 b	52.8±3.3 a	34.4±2.5

地区	当年萌芽率/%	污染率/%	第2年萌芽率/%	第2年污染率/%
山东	14.9±1.8 c	63.8±3.3 a	31.7±2.4 d	54.8±2.9
河南	12.4±2.2 c	61.3±4.5 a	33.4±2.0 cd	57.0±1.6
江苏	20.2±1.8 b	65.4±1.9 a	36.9±1.0 bc	55.0±1.6
浙江	23.8±2.8 b	62.8±2.1 a	40.4±1.5 b	49.2±0.7
广西	43.5±1.9 a	40.0±4.9 b	52.8±3.3 a	34.4±2.5

贮藏方式	染色率/%		发芽率/%	污染率/%
贮藏方式	0.1%TTC	0.5%TTC	发芽率/%	污染率/%
沙藏	62.6±3.3	75.2±2.8	63.5±2.9	56.2±2.2
冷藏	56.4±1.4	59.8±1.5	51.6±1.3	42.5±2.3
冷冻	3.8±6.6	3.8±6.6	0±0	69.5±5.4
对照	36.8±2.4	40.8±3.6	44.4±2.1	44.4±2.1