Study on the metabolism regularity of diazepam in Parabramis pekinensis

doi:10.16178/j.issn.0528-9017.20250032

Abstract

Abstract:

A method based on ultra performance liquid chromatography electrostatic field orbital high resolution mass spectrometry (UPLC-Q Exactive MS) was developed to study the enrichment and metabolic patterns of diazepam in Parabramis pekinensis. By conducting an experiment on the distribution of diazepam in the tissue of the fish, the transmission path of diazepam in the fish body was determined. By studying the metabolism of diazepam in Parabramis pekinensis, the fastest detection time, concentration, and metabolic patterns of diazepam in Parabramis pekinensis were determined. The metabolic period of diazepam in Parabramis pekinensis is quite long. In the actual production process, it is important to avoid introducing diazepam into the water and fish as much as possible. Water and aquatic products should be monitored irregularly to ensure the safety and health of consumers.

Key words: Parabramis pekinensis, diazepam, enrichment, metabolic patterns

CLC Number:

S859.84

WANG Jieqiong, YIN Xueyan, LIU Xiao, WANG Yuqing, ZHAI Yunzhong, GU Xiaoming, WANG Yakun. Study on the metabolism regularity of diazepam in Parabramis pekinensis[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2468-2472.

Figures/Tables 5

References 20

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步骤	开始时间/ min	步骤时长/ s	Loading泵				Eluting泵
步骤	开始时间/ min	步骤时长/ s	流速/(mL·min^-1)	A/%	B/%	C/%	转变模式	流速/(mL·min^-1)	A/%	B/%
1	0.00	30	2.00	100	—	—	Step	0.40	100	—
2	0.50	60	0.10	100	—	—	Step	0.40	95	5
3	1.50	60	0.50	100	—	—	Step	0.40	50	50
4	2.50	60	0.50	—	—	100	Ramp	0.40	30	70
5	3.50	300	0.50	—	—	100	Ramp	0.40	10	90
6	8.50	150	1.00	70	30	—	Ramp	0.40	95	5
7	11.00	300	1.00	100	—	—	Step	0.40	100	—

步骤	开始时间/ min	步骤时长/ s	Loading泵				Eluting泵
步骤	开始时间/ min	步骤时长/ s	流速/(mL·min^-1)	A/%	B/%	C/%	转变模式	流速/(mL·min^-1)	A/%	B/%
1	0.00	30	2.00	100	—	—	Step	0.40	100	—
2	0.50	60	0.10	100	—	—	Step	0.40	95	5
3	1.50	60	0.50	100	—	—	Step	0.40	50	50
4	2.50	60	0.50	—	—	100	Ramp	0.40	30	70
5	3.50	300	0.50	—	—	100	Ramp	0.40	10	90
6	8.50	150	1.00	70	30	—	Ramp	0.40	95	5
7	11.00	300	1.00	100	—	—	Step	0.40	100	—

时间/h	A组(48 μg·kg^-1)体内地西泮/(μg·L^-1)				B组(30 μg·kg^-1)体内地西泮/(μg·L^-1)
时间/h	血浆	肝脏	肾脏	肌肉	血浆	肝脏	肾脏	肌肉
0.5	32.2	35.5	20.3	12.6	21.7	37.7	16.7	10.1
1.0	24.6	67.7	31.2	10.2	17.7	39.3	28.6	13.9
2.0	26.5	81.0	41.2	25.8	20.9	49.2	35.4	12.4
4.0	30.3	57.2	53.2	39.8	18.6	41.1	39.0	14.9
6.0	25.2	61.7	37.9	29.9	12.3	41.3	30.0	15.6
10.0	22.4	55.7	41.6	21.6	10.1	33.1	35.0	11.2
24.0	16.5	38.7	37.5	23.6	14.9	24.7	30.5	13.0

时间/h	A组(48 μg·kg^-1)体内地西泮/(μg·L^-1)				B组(30 μg·kg^-1)体内地西泮/(μg·L^-1)
时间/h	血浆	肝脏	肾脏	肌肉	血浆	肝脏	肾脏	肌肉
0.5	32.2	35.5	20.3	12.6	21.7	37.7	16.7	10.1
1.0	24.6	67.7	31.2	10.2	17.7	39.3	28.6	13.9
2.0	26.5	81.0	41.2	25.8	20.9	49.2	35.4	12.4
4.0	30.3	57.2	53.2	39.8	18.6	41.1	39.0	14.9
6.0	25.2	61.7	37.9	29.9	12.3	41.3	30.0	15.6
10.0	22.4	55.7	41.6	21.6	10.1	33.1	35.0	11.2
24.0	16.5	38.7	37.5	23.6	14.9	24.7	30.5	13.0

总试验天数/d	代谢时间/d	鳊鱼中地西泮含量/(μg·kg^-1)			水温/ ℃
总试验天数/d	代谢时间/d	B处理	C处理	D处理	水温/ ℃
10	3	1.860	2.469	2.809	22
12	5	1.806	2.170	2.627	23
14	7	1.628	1.929	2.295	22
17	10	1.705	2.088	2.415	23
22	15	1.502	1.798	1.919	24
27	20	1.364	1.608	1.843	23
32	25	1.159	1.471	1.627	23
37	30	1.003	1.298	1.466	25
44	37	0.926	1.104	1.372	23
51	44	0.812	0.925	1.107	24
58	51	0.703	0.797	0.896	28
67	58	0.609	0.699	0.785	26
72	65	0.612	0.700	0.712	28
79	72	0.569	0.621	0.704	27
86	79	0.508	0.581	0.604	28
93	86	0.441	0.494	0.528	29
100	93	0.409	0.430	0.460	28
107	100	0.374	0.393	0.425	28