Analysis of the indicative role of active ingredients in the roots of Rubus chingii Hu on the quality of immature fruits

doi:10.16178/j.issn.0528-9017.20250462

Abstract

Abstract:

In this study, high-performance liquid chromatography (HPLC) was applied to determine the active ingredients in the roots and immature fruits of Rubus chingii Hu. Correlation analyses were conducted between the contents of ellagic acid and gallic acid in roots and the contents of ellagic acid and kaempferol-3-O-rutinoside in immature fruits respectively. The results showed that the content of ellagic acid in the roots of Rubus chingii Hu was 0.88%-2.02%, and the content of gallic acid was 0.52%-1.10%. The content of ellagic acid in immature fruits was 0.07%-0.32%, and the content of kaempferol-3-O-rutinoside was 0.03%-0.16%. The total content of ellagic acid and gallic acid in roots showed a linear correlation with the content of ellagic acid in immature fruits, with the determination coefficient R² being 0.740 9. When the total content of ellagic acid and gallic acid in roots was higher than 2.60%, the predicted value of ellagic acid content in immature fruits was higher than the index requirement of Pharmacopoeia of the People's Republic of China. No linear correlation was found between the contents of ellagic acid and gallic acid in roots and the content of kaempferol-3-O-rutinoside in immature fruits. This study indicates that determining the contents of ellagic acid and gallic acid in roots during the dormancy period can be used for pre-screening breeding materials with high levels of ellagic acid in fruits.

Key words: Rubus chingii Hu, ellagic acid, gallic acid, kaempferol-3-O-rutinoside

CLC Number:

S685.12

WANG Chuanbao, JIANG Zhiliang, HU Chunli, YU Guoliang, ZHAN Jianyong, JIANG Ling, ZHENG Pinghan, WANG Zhian, SUN Jian. Analysis of the indicative role of active ingredients in the roots of Rubus chingii Hu on the quality of immature fruits[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2352-2358.

Figures/Tables 7

Table 1 Average recovery of ellagic acid and kaempferol-3-O-rutinoside in fruits of Rubus chingii Hu

成分	样品中含量/mg	加入量/mg	检测量/mg	加样回收率/%	平均回收率/%	RSD/%
鞣花酸	1.311 2	1.320 0	2.621 2	99.24	100.46	1.33
	1.295 8	1.320 0	2.650 1	102.60
	1.310 5	1.320 0	2.621 0	99.28
	1.311 3	1.320 0	2.630 4	99.93
	1.302 3	1.320 0	2.642 1	101.50
	1.310 1	1.320 0	2.632 5	100.18
山柰酚-3-O-芸香糖苷	0.205 5	0.210 0	0.420 1	102.19	101.50	1.46
	0.210 3	0.210 0	0.421 4	100.52
	0.200 6	0.210 0	0.418 5	103.76
	0.211 1	0.210 0	0.423 0	100.90
	0.210 4	0.210 0	0.419 6	99.62
	0.211 4	0.210 0	0.425 6	102.00

Fig.1 Liquid chromatography of test sample and control sample in roots of Rubus chingii Hu

Fig.2 Liquid chromatography of test sample and control sample in fruits of Rubus chingii Hu

Fig.3 Contents of indicator ingredients in Rubus chingii Hu sample

Fig.4 Histogram of frequency distribution of indicator ingredients in Rubus chingii Hu sample

Fig.5 Cluster heatmap of indicator ingredients in the roots and fruits of Rubus chingii Hu

Fig.6 Linear analysis of indicator ingredients in the roots and fruits of Rubus chingii Hu

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