Detection of the contents of six iridoids and penylpropanoids in Scrophularia ninggpoensis Hemsl. and study on their anti-inflammatory activities

doi:10.16178/j.issn.0528-9017.20250494

Abstract

Abstract:

This study aims to analyze the content distribution patterns of six components in Scrophularia ninggpoensis Hemsl. and explore their correlation with anti-inflammatory activity. The contents of aucubin, harpagide, verbascoside, angloside C, harpagoside and cinnamic acid in 12 batches of Scrophularia ninggpoensis Hemsl. samples were determined by high performance liquid chromatography. Meanwhile, the anti-inflammatory activity of different Scrophularia ninggpoensis Hemsl. samples was evaluated using the RAW264.7 cell (mouse mononuclear macrophage) model. Through principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), cluster analysis and correlation analysis, the chemical composition and anti-inflammatory activity of the samples were systematically analyzed to screen out the key differential compounds and their anti-inflammatory related components. The results showed that the content ranges of each component in Scrophularia ninggpoensis Hemsl. were as follows: the content of aucubin was 0.80% to 1.54%, the content of harpagide was 0.65% to 1.92%, the content of verbascoside was 0.05% to 0.47%, the content of angloside C was 0.25% to 0.52%, and the content of harpagoside was 0.19% to 0.61%. The content of cinnamic acid was 0.01% to 0.07%, and the NO inhibition rates of different samples ranged from 22.33% to 40.76%. The results of principal component analysis and partial least squares discriminant analysis showed that there were obvious differences among different samples, with the main differential compounds being cinnamic acid, aucubin and harpagide. Cluster analysis and correlation analysis indicated that harpagide and verbascoside were positively correlated with anti-inflammatory activity. Through the determination of the active components and anti-inflammatory activities of Scrophularia ninggpoensis Hemsl., the differential compounds and anti-inflammatory related components were identified, providing a theoretical basis for the inclusion of penylpropanoids in the quality control of Scrophularia ninggpoensis Hemsl..

Key words: Scrophularia ninggpoensis Hemsl., iridoids, penylpropanoids, anti-inflammatory activities, quality control

CLC Number:

S567

ZHANG Minda, LI Haishen, SUN Jian, WANG Pan, QIU Ping, YU Guoliang, ZHOU Chaobin, WANG Zhian. Detection of the contents of six iridoids and penylpropanoids in Scrophularia ninggpoensis Hemsl. and study on their anti-inflammatory activities[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2455-2461.

Figures/Tables 5

Fig.1 HPLC contents of six components in Scrophularia ninggpoensis Hemsl. samples

Table 1 Cytotoxicity of different concentrations of Scrophularia ninggpoensis Hemsl. extracts

浓度/(μg·mL^-1)	吸光度	相对标准偏差/%	细胞存活率/%
0	0.428 9	3.67	—
12.5	0.400 8	3.17	93.44
25.0	0.386 2	5.55	90.04
50.0	0.397 3	7.96	92.64
100.0	0.405 1	5.38	94.45
200.0	0.398 7	4.17	92.95
400.0	0.402 9	5.89	93.95

Fig.2 The anti-inflammatory activity of different samples of Scrophularia ninggpoensis Hemsl.

Fig.3 PCA and PLS-DA results of different Scrophularia ninggpoensis Hemsl. Samples

Fig.4 Clustering heat maps and correlation analysis results of different Scrophularia ninggpoensis Hemsl. Samples

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