Effect on seed germination and seedling growth of soaking seeds with diethyl aminoethyl hexanoate in Cynanchum bungei Decne.

doi:10.16178/j.issn.0528-9017.20250578

Abstract

Abstract:

Cynanchum bungei Decne. is a characteristic medicinal and edible crop in Binhai County, Jiangsu Province, which is of great significance to the development of local agricultural economy. This study systematically investigated the mechanism of the plant growth regulator diethyl aminoethyl hexanoate (DA-6) on seed germination and seedling growth of Cynanchum bungei. The results indicated that seed soaking with DA-6 significantly (p<0.05) enhanced germination rate, germination energy, germination index, and vigor index, while also promoting seedling height, stem diameter, root length, and fresh weight. Physiological analyses revealed that DA-6 effectively increased the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and reduced malondialdehyde (MDA) content. It is noteworthy that the seed soaking treatment with DA-6 at a concentration of 15 mg·L^-1 demonstrated the optimal effect, with all indicators significantly better than those of the control group. The comprehensive analysis revealed that DA-6 may promote the growth and development of Cynanchum bungei by regulating the antioxidant system and reducing membrane lipid peroxidation damage, which provides a theoretical basis for the efficient cultivation of Cynanchum bungei.

Key words: Cynanchum bungei Decne., diethyl aminoethyl hexanoate, seed germination, seedling growth

CLC Number:

S567.7

WU Chengdong, SHEN Mingchen, ZHANG Ming, YAN Kaixuan, ZHU Li, CHEN Lei. Effect on seed germination and seedling growth of soaking seeds with diethyl aminoethyl hexanoate in Cynanchum bungei Decne.[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(11): 2680-2684.

Figures/Tables 3

References 17

[1]	印鑫, 丁永芳, 邵久针, 等. 白首乌的研究进展[J]. 中草药, 2019, 50(4): 992-1000.
[2]	黄文婷, 冯乃杰, 郑殿峰, 等. 烯效唑和胺鲜酯对大豆叶片光合特性与碳代谢的调控效应[J]. 大豆科学, 2020, 39(2): 243-251.
[3]	王晨霞, 任如佳, 常瑞雪, 等. 生物活性物质提高玉米种子萌发及抗盐能力[J]. 中国农业大学学报, 2020, 25(7): 20-27.
[4]	王铎, 田雨龙, 张鸿建, 等. 胺鲜酯对铬胁迫下白三叶种子萌发特性的影响[J]. 草业科学, 2021, 38(10): 1986-1997.
[5]	张雪梅, 王雨熙, 朱沿舟, 等. 胺鲜酯对树番茄幼苗养分吸收和生理指标的影响[J]. 云南农业大学学报(自然科学), 2023, 38(4): 606-614.
[6]	GIANNOPOLITIS C N, RIES S K. Superoxide dismutases Ⅰ: occurrence in higher plants[J]. Plant Physiology, 1977, 59(2): 309-314.
[7]	MANO J, OHNO C, DOMAE Y, et al. Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photooxidative stress in spinach leaves: its relevance to monodehydroascorbate radical detected with in vivo ESR[J]. Biochimica et Biophysica Acta:Bioenergetics, 2001, 1504(2/3): 275-287.
[8]	CAKMAK I, MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant Physiology, 1992, 98(4): 1222-1227.
[9]	HEATH R L, PACKER L. Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics, 1968, 125(1): 189-198.
[10]	王文浩, 王丹, 宋贤鹏, 等. 植物生长调节剂复配对棉花生长及内源激素的影响[J]. 农药学学报, 2023, 25(5): 1093-1103.
[11]	周澜. 胺鲜酯对不同氮水平下绿豆生理和产量的调控效应[D]. 大庆: 黑龙江八一农垦大学, 2025.
[12]	曹亦芹, 程碧真, 李州. 胺鲜酯(DA-6)浸种对盐胁迫下白三叶种子萌发及抗盐性的影响[J]. 草地学报, 2023, 31(1): 140-147.
[13]	吴挺. 胺鲜酯·芸苔素内酯对水稻生长及产量的影响[J]. 上海农业科技, 2019(4): 48-49, 54.
[14]	满振峰, 方淑梅, 王庆燕, 等. 胺鲜酯与噻苯隆及其复配对大豆产量和品质的调控效应[J]. 黑龙江八一农垦大学学报, 2024, 36(5): 1-7.
[15]	崔灿, 王梦琦, 赵琬璐, 等. 胺鲜酯浸种对NaCl胁迫下紫花苜蓿种子萌发及幼苗生长的影响[J]. 草业学报, 2025, 34(6): 46-58.
[16]	阎晓光, 杜艳伟, 李洪, 等. 不同浓度胺鲜酯处理吐丝期玉米自交系对种子活力的影响[J]. 种子, 2020, 39(11): 11-13.
[17]	毛桂玲, 李梅兰, 任毛飞. 三种肥料增效剂对黄瓜幼苗生长的影响[J]. 北方园艺, 2021(18): 1-6.

DA-6质量浓度/(mg·L^-1)	发芽率/%	发芽势/%	发芽指数	活力指数
0	66.00±3.06 d	50.00±2.89 c	6.00±0.35 d	0.92±0.08 d
5	75.33±2.52 c	60.00±2.08 b	8.25±0.42 c	1.56±0.12 c
10	78.00±2.08 b	63.33±1.53 b	8.78±0.38 b	1.82±0.15 b
15	86.67±1.53 a	76.67±1.53 a	10.56±0.49 a	2.83±0.21 a
20	83.33±2.08 ab	70.00±2.08 a	9.58±0.45 ab	2.25±0.18 ab
25	80.00±2.08 b	66.67±2.08 ab	9.06±0.42 b	1.98±0.16 b

DA-6质量浓度/(mg·L^-1)	发芽率/%	发芽势/%	发芽指数	活力指数
0	66.00±3.06 d	50.00±2.89 c	6.00±0.35 d	0.92±0.08 d
5	75.33±2.52 c	60.00±2.08 b	8.25±0.42 c	1.56±0.12 c
10	78.00±2.08 b	63.33±1.53 b	8.78±0.38 b	1.82±0.15 b
15	86.67±1.53 a	76.67±1.53 a	10.56±0.49 a	2.83±0.21 a
20	83.33±2.08 ab	70.00±2.08 a	9.58±0.45 ab	2.25±0.18 ab
25	80.00±2.08 b	66.67±2.08 ab	9.06±0.42 b	1.98±0.16 b

DA-6质量浓度/(mg·L^-1)	株高/cm	茎粗/cm	根长/cm	鲜重/g
0	9.20±0.52 d	0.22±0.02 d	7.60±0.42 d	0.13±0.01 d
5	10.45±0.42 c	0.24±0.02 c	8.45±0.38 c	0.16±0.01 c
10	10.95±0.49 b	0.25±0.02 c	8.85±0.45 b	0.18±0.01 b
15	12.58±0.57 a	0.29±0.02 a	10.45±0.53 a	0.27±0.02 a
20	12.30±0.53 a	0.28±0.02 a	10.20±0.48 a	0.26±0.02 a
25	11.35±0.50 b	0.26±0.02 b	9.25±0.42 b	0.20±0.01 b

DA-6质量浓度/(mg·L^-1)	株高/cm	茎粗/cm	根长/cm	鲜重/g
0	9.20±0.52 d	0.22±0.02 d	7.60±0.42 d	0.13±0.01 d
5	10.45±0.42 c	0.24±0.02 c	8.45±0.38 c	0.16±0.01 c
10	10.95±0.49 b	0.25±0.02 c	8.85±0.45 b	0.18±0.01 b
15	12.58±0.57 a	0.29±0.02 a	10.45±0.53 a	0.27±0.02 a
20	12.30±0.53 a	0.28±0.02 a	10.20±0.48 a	0.26±0.02 a
25	11.35±0.50 b	0.26±0.02 b	9.25±0.42 b	0.20±0.01 b

DA-6质量浓度/ (mg·L^-1)	SOD活性/ (U·g^-1)	POD活性/ (U·g^-1·min^-1)	CAT活性/ (U·g^-1·min^-1)	MDA含量/ (μmol·g^-1)
0	165.67±5.86 d	123.25±4.32 d	71.00±3.25 d	9.14±0.42 a
5	192.33±6.25 c	145.50±5.18 c	82.67±3.86 c	7.86±0.35 b
10	210.50±5.67 b	162.75±4.89 b	89.33±3.52 b	7.02±0.28 d
15	235.67±6.50 a	186.33±5.67 a	98.50±4.12 a	6.25±0.25 e
20	228.33±5.92 a	178.67±5.25 a	94.33±3.98 a	6.58±0.28 e
25	205.00±5.75 b	156.25±4.67 b	85.67±3.65 b	7.35±0.32 c