Journal of Zhejiang Agricultural Sciences ›› 2025, Vol. 66 ›› Issue (12): 3036-3043.DOI: 10.16178/j.issn.0528-9017.20240443

Previous Articles     Next Articles

Research and application of RNA interference in the prevention and control of agricultural and forestry pests and diseases

CUI Can1,2(), LI Xiaojuan1,2,*()   

  1. 1. Anhui Academy of Forestry, Hefei 230088, Anhui
    2. Key Laboratory of National Forestry and Grassland Administration on Prevention and Control Technology of Pine Wilt Disease, Hefei 230031, Anhui
  • Received:2024-05-30 Online:2025-12-11 Published:2025-12-17

Abstract:

RNA interference (RNAi) is a biological process that utilizes double-stranded RNA or small interfering RNA to specifically degrade or inhibit the expression of target gene mRNA. In recent years, due to its high specificity and selectivity, RNAi has been widely applied in the research and control of agricultural and forestry pests and diseases. This paper systematically summarized the main application approaches of RNAi in agricultural and forestry pest and disease control, including spray-induced gene silencing, virus-induced gene silencing, host-induced gene silencing, and nanocarriers delivered gene silencing. It also summarized the latest research progress and current application of RNAi in this field, discussed prominent challenges according to existing studies, and proposed corresponding recommendations. Finally, future development directions for RNAi in the prevention and control of agricultural and forestry pests and diseases are outlined. RNAi-based prevention and control strategies for agricultural and forestry pests and diseases are expected to bring new vitality to the protection of agricultural and forestry crops, thereby promoting the sustainable development of agriculture and forestry.

Key words: RNA interference, agricultural and forestry pests and disease, target gene

CLC Number: