Journal of Zhejiang Agricultural Sciences ›› 2023, Vol. 64 ›› Issue (7): 1768-1773.DOI: 10.16178/j.issn.0528-9017.20230379
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Received:2023-04-25
Online:2023-07-11
Published:2023-07-04
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URL: http://www.zjnykx.cn/EN/10.16178/j.issn.0528-9017.20230379
| 基因 | 上游引物 | 下游引物 | 片段大小/bp | 内切酶 | |||||
|---|---|---|---|---|---|---|---|---|---|
| Pi37 | ATTCACGCAACCAGGGGGATA | TCGCAACTCATATCCCTTCTG | 716(R) | — | |||||
| Pit | ATGATAACCTCATCCTCAATAAGT | AAGCACCAAGTGGCGCCA | 590(S) | — | |||||
| Pish | GATCGATCATGGCCAGAGAG | ACAGCAGGTTGATGAGGACC | 207(R) | — | |||||
| Pi35 | GCGTACAGCGTTCGCTCTATA | CTTCAAAAGCTCACAACCA | |||||||
| CCGTCCTCCCTCCAGCATATATGTAT | CTGCAAAACAAAGGAAACGTG | 535+317(R) | — | ||||||
| AAGGGATGCAGGATAAAGAATGAA | 535+266(S) | — | |||||||
| Pi64 | TCCTGTGTTTCCTACCGAGTCCAGC | AGAGGAGTGCAAGGTTACCAGAGCC | 1016(R) | — | |||||
| Pib | GAACAATGCCCAAACTTGAGA | GGGTCCACATGTCAGTGAGC | 365(R) | — | |||||
| Pi63 | TGGCTATCTGGTCTCCGTG | TCCAGATCAGGGCAGTTAGA | 614(R) | — | |||||
| Pi2 | CAGCGATGGTATGAGCACAA | CGTTCCTATACTGCCACATCG | 450(R)/282(S) | — | |||||
| Pi9 | GCTGTGCTCCAAATGAGGAT | GCGATCTCACATCCTTTGCT | 397(S)/291(R) | — | |||||
| Pizt | ATGTGGATGCTGTGTTAT | TAGTTTGCTGCTCAATAAGTA | 176(R) | — | |||||
| Pi50 | TTGCACATGGTGCTGGATGG | GATCTTCCTCGAAAGAGCTG | 1260(R) | — | |||||
| Pi36 | ATCATGGCAAATACTCGAAAG | CATATCATGAACACGACAACC | 628(R) | — | |||||
| Pi5 | ATAGATCATGCGCCCTCTTG | TCATACCCCATTCGGTCATT | 307(S)/206(R) | — | |||||
| Pi56 | TGCAAGAACCCTCCTCCTAC | CCTGCCATCTCAAGACTCTC | 725(S) | — | |||||
| Pita | GCAGGTTATAAGCTAGCTAT | GCCAAATAGCCAATTCATA | 240+179(S) | — | |||||
| CAAGAGAGAGTATTTGCTTAGGAGT | CCTATATCTCTTTAAAATATGTTTGGC | 240+104(R) | — | ||||||
| Pid3 | TACTACTCATGGAAGCTAGTTCTC | AGCACTTCTTGACTACTGTCTGCC | 658 | BamHI | |||||
| Pi1 | GGTTGGTCGAAACCAGAAAA | GCTGAGGTAGAAGCGGGAGC | 220(R)/209(S) | — | |||||
| Pia | GCTCCTTCTTCAATTACTGGGAATGGTCGA | CTTGCAGTATCTCAAACTGG | 119(S)/89(R) | SalI | |||||
| Pid4 | CCGCATCTCCTCCTTCCTCT | AGAGATCTCGCTGTTCAGTC | 352(S)/279(R) | — | |||||
| Pik | GGAAAGCTGATATGTTGTCG | ACTCGGAGTCGGAGAGTCAG | 1650(R) | — | |||||
| Pikm2 | CAGTAGCTGTGTCTCAGAACTATG | AAGGTACCTCTTTTCGGCCAG | 332(S)/290(R) | — | |||||
| Pigm | CCTTGTTCCTCCTGCTATC | ATCTCGCTGTTCAGTCTTG | 220(R) | — | |||||
| Ptr | TGCAGATTTGACTGCTCGGT | GGGATCTTCCTCGCCCAAA | 200(R) | — | |||||
| Pikm2(测序) | GAGATCCTCCGCAATTTACC | TACTACGGGACCATGGTAAG | 601 | — | |||||
| Xa5 | CCGGAGCTCGCCATTCAAGTTCTTG | TGCTCTTGACTTGGTTCTCC | 170(S)/145(R) | — | |||||
| Xa10 | CGCAATCACTTCAATTACTT | GAAGAATGAAAGAGGAGGG | 270(R) | — | |||||
| Xa13 | AGCTCCAGCTCTCCAAATG | GGCCATGGCTCAGTGTTTAT | 490(R)/281(S) | — | |||||
| Xa21 | CGATCGGTATAACAGCAAAAC | TCTGATCATGCATGTTCTGTG | 574(R)/447(S) | — | |||||
| Xa23 | CTCTTCCCTGAGTCAAAGTC | GGAGAATAACCATCTTGTCG | 640(R) | — | |||||
| Xa1 | CACATCACGGCCTCAAGTATCT | TCTCCATAACCACTGATTGCG | 472(R) | — | |||||
| Xa7 | GGGAGAAATTGGCCCCAGTTAGAGAA | GCTTCACTTGATCACTGCTGTTTG | 531(S)/306(R) | — | |||||
| STV11(测序) | CTGCACAGTGCACACGATAC | ATAATAGATAGATAGATAGATA | 1222 | — | |||||
| STV11 | GACATGGCCGAGCTCATC | GGAGATGGCGAAGGTGGA | 312(S) | SmaI | |||||
| 211+101(R) | |||||||||
| STV11 | TGAAGGGTGTCACAACGAGC | GGAGGAAGAGGACCTTCTC | 180(S)/169(R) | SacI | |||||
| Xa25(测序) | CACTACATCTCTTCCCTTCA | CTTGTCTCCTCCAAGTTTGC | 1154 | — | |||||
| Xa14 | GGCTTCCTTCTTCACTTACCTGT | GCAGCGTTTTTAGGGAATCA | 608(R) | — | |||||
| 基因 | 上游引物 | 下游引物 | 片段大小/bp | 内切酶 | |||||
|---|---|---|---|---|---|---|---|---|---|
| Pi37 | ATTCACGCAACCAGGGGGATA | TCGCAACTCATATCCCTTCTG | 716(R) | — | |||||
| Pit | ATGATAACCTCATCCTCAATAAGT | AAGCACCAAGTGGCGCCA | 590(S) | — | |||||
| Pish | GATCGATCATGGCCAGAGAG | ACAGCAGGTTGATGAGGACC | 207(R) | — | |||||
| Pi35 | GCGTACAGCGTTCGCTCTATA | CTTCAAAAGCTCACAACCA | |||||||
| CCGTCCTCCCTCCAGCATATATGTAT | CTGCAAAACAAAGGAAACGTG | 535+317(R) | — | ||||||
| AAGGGATGCAGGATAAAGAATGAA | 535+266(S) | — | |||||||
| Pi64 | TCCTGTGTTTCCTACCGAGTCCAGC | AGAGGAGTGCAAGGTTACCAGAGCC | 1016(R) | — | |||||
| Pib | GAACAATGCCCAAACTTGAGA | GGGTCCACATGTCAGTGAGC | 365(R) | — | |||||
| Pi63 | TGGCTATCTGGTCTCCGTG | TCCAGATCAGGGCAGTTAGA | 614(R) | — | |||||
| Pi2 | CAGCGATGGTATGAGCACAA | CGTTCCTATACTGCCACATCG | 450(R)/282(S) | — | |||||
| Pi9 | GCTGTGCTCCAAATGAGGAT | GCGATCTCACATCCTTTGCT | 397(S)/291(R) | — | |||||
| Pizt | ATGTGGATGCTGTGTTAT | TAGTTTGCTGCTCAATAAGTA | 176(R) | — | |||||
| Pi50 | TTGCACATGGTGCTGGATGG | GATCTTCCTCGAAAGAGCTG | 1260(R) | — | |||||
| Pi36 | ATCATGGCAAATACTCGAAAG | CATATCATGAACACGACAACC | 628(R) | — | |||||
| Pi5 | ATAGATCATGCGCCCTCTTG | TCATACCCCATTCGGTCATT | 307(S)/206(R) | — | |||||
| Pi56 | TGCAAGAACCCTCCTCCTAC | CCTGCCATCTCAAGACTCTC | 725(S) | — | |||||
| Pita | GCAGGTTATAAGCTAGCTAT | GCCAAATAGCCAATTCATA | 240+179(S) | — | |||||
| CAAGAGAGAGTATTTGCTTAGGAGT | CCTATATCTCTTTAAAATATGTTTGGC | 240+104(R) | — | ||||||
| Pid3 | TACTACTCATGGAAGCTAGTTCTC | AGCACTTCTTGACTACTGTCTGCC | 658 | BamHI | |||||
| Pi1 | GGTTGGTCGAAACCAGAAAA | GCTGAGGTAGAAGCGGGAGC | 220(R)/209(S) | — | |||||
| Pia | GCTCCTTCTTCAATTACTGGGAATGGTCGA | CTTGCAGTATCTCAAACTGG | 119(S)/89(R) | SalI | |||||
| Pid4 | CCGCATCTCCTCCTTCCTCT | AGAGATCTCGCTGTTCAGTC | 352(S)/279(R) | — | |||||
| Pik | GGAAAGCTGATATGTTGTCG | ACTCGGAGTCGGAGAGTCAG | 1650(R) | — | |||||
| Pikm2 | CAGTAGCTGTGTCTCAGAACTATG | AAGGTACCTCTTTTCGGCCAG | 332(S)/290(R) | — | |||||
| Pigm | CCTTGTTCCTCCTGCTATC | ATCTCGCTGTTCAGTCTTG | 220(R) | — | |||||
| Ptr | TGCAGATTTGACTGCTCGGT | GGGATCTTCCTCGCCCAAA | 200(R) | — | |||||
| Pikm2(测序) | GAGATCCTCCGCAATTTACC | TACTACGGGACCATGGTAAG | 601 | — | |||||
| Xa5 | CCGGAGCTCGCCATTCAAGTTCTTG | TGCTCTTGACTTGGTTCTCC | 170(S)/145(R) | — | |||||
| Xa10 | CGCAATCACTTCAATTACTT | GAAGAATGAAAGAGGAGGG | 270(R) | — | |||||
| Xa13 | AGCTCCAGCTCTCCAAATG | GGCCATGGCTCAGTGTTTAT | 490(R)/281(S) | — | |||||
| Xa21 | CGATCGGTATAACAGCAAAAC | TCTGATCATGCATGTTCTGTG | 574(R)/447(S) | — | |||||
| Xa23 | CTCTTCCCTGAGTCAAAGTC | GGAGAATAACCATCTTGTCG | 640(R) | — | |||||
| Xa1 | CACATCACGGCCTCAAGTATCT | TCTCCATAACCACTGATTGCG | 472(R) | — | |||||
| Xa7 | GGGAGAAATTGGCCCCAGTTAGAGAA | GCTTCACTTGATCACTGCTGTTTG | 531(S)/306(R) | — | |||||
| STV11(测序) | CTGCACAGTGCACACGATAC | ATAATAGATAGATAGATAGATA | 1222 | — | |||||
| STV11 | GACATGGCCGAGCTCATC | GGAGATGGCGAAGGTGGA | 312(S) | SmaI | |||||
| 211+101(R) | |||||||||
| STV11 | TGAAGGGTGTCACAACGAGC | GGAGGAAGAGGACCTTCTC | 180(S)/169(R) | SacI | |||||
| Xa25(测序) | CACTACATCTCTTCCCTTCA | CTTGTCTCCTCCAAGTTTGC | 1154 | — | |||||
| Xa14 | GGCTTCCTTCTTCACTTACCTGT | GCAGCGTTTTTAGGGAATCA | 608(R) | — | |||||
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