Study on tissue culture and rapid propagation of Hydrangea macrophylla cv. Ivetta

doi:10.16178/j.issn.0528-9017.20230500

Abstract

Abstract:

In order to establish a large-scale propagation and efficient genetic transformation system of Hydrangea macrophylla cv. Ivetta, the effects of different disinfection methods, basic medium types, hormone types and concentrations, transplanting substrate and other factors on adventitious bud induction, rooting and transplanting survival rate were studied by using Ivetta with bud shoots as explants. The results showed that Ivetta was disinfected by NaClO with an effective chlorine concentration of 2% for 10 min, and the contamination rate was low, and the optimal medium for axillary bud initiation was 1/2 B5+2.0 mg·L^-1 6-BA; The optimal proliferation medium was B5+2.0 mg·L^-1 6-BA; The optimal rooting medium was 1/2 B5+0.5 mg·L^-1 IBA; The most suitable substrate for transplanting sterile seedlings was perlite:peat soil=1:1 (V:V). In this study, the tissue culture and rapid propagation technology system of Ivetta was preliminarily established, which could provide technical support for its factory seedling breeding and large-scale production.

Key words: Hydrangea macrophylla, Ivetta, tissue culture, rapid propagation

CLC Number:

S682.1

CHEN Shuangshuang, QIN Ziyi, QIU Shuai, FENG Jing, GAO Kai, QI Xiangyu, CHEN Huijie, DENG Yanming. Study on tissue culture and rapid propagation of Hydrangea macrophylla cv. Ivetta[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(7): 1627-1633.

Figures/Tables 10

References 23

[1]	任倩倩, 郑建鹏, 张京伟, 等. 绣球属抗逆性研究进展[J]. 安徽农业科学, 2020, 48(11): 26-28.
[2]	闵祥凤, 姜卫兵, 魏家星. “绣球” 类观赏植物资源探究及应用开发[J]. 中国城市林业, 2017, 15(1): 60-63.
[3]	冯卫生, 张艳丽, 郑晓珂, 等. 绣球花的化学成分研究[J]. 中国药学杂志, 2011, 46(8): 576-579.
[4]	邓衍明, 韩勇, 齐香玉, 等. 绣球属植物种质资源分析及其花色可调性和叶斑病抗性比较[J]. 植物资源与环境学报, 2018, 27(4): 90-100.
[5]	陈双双, 齐香玉, 冯景, 等. 铝处理下绣球实时荧光定量PCR内参基因筛选及验证[J]. 华北农学报, 2021, 36(2): 9-18.
[6]	陈双双, 齐香玉, 冯景, 等. 基于流式细胞术和基因组Survey的绣球基因组大小及特征分析[J]. 江苏农业科学, 2021, 49(12): 39-44.
[7]	陈慧杰, 邓衍明, 齐香玉, 等. 绣球叶斑病病原鉴定及其生物学特性[J]. 江苏农业科学, 2022, 50(7): 106-111.
[8]	冯景, 邓衍明, 齐香玉, 等. 圆锥绣球‘石灰灯’种子形态及萌发特性研究[J]. 江苏林业科技, 2022, 49(3): 9-14.
[9]	CHEN S S, QI X Y, FENG J, et al. Biochemistry and transcriptome analyses reveal key genes and pathways involved in high-aluminum stress response and tolerance in Hydrangea sepals[J]. Plant Physiology and Biochemistry, 2022, 185: 268-278.
[10]	QIN Z Y, CHEN S S, FENG J, et al. Identification of aluminum-activated malate transporters (ALMT) family genes in Hydrangea and functional characterization of HmALMT5/9/11 under aluminum stress[J]. PeerJ, 2022, 10: e13620.
[11]	QI X Y, CHEN S S, WANG H D, et al. Comparative physiology and transcriptome analysis reveals that chloroplast development influences silver-white leaf color formation in Hydrangea macrophylla var. Maculata[J]. BMC Plant Biology, 2022, 22(1): 345.
[12]	LIU F, HUANG L L, LI Y L, et al. Shoot organogenesis in leaf explants of Hydrangea macrophylla ‘Hyd1’ and assessing genetic stability of regenerants using ISSR markers[J]. Plant Cell, Tissue and Organ Culture, 2011, 104(1): 111-117.
[13]	殷丽青, 胡永红, 汤桂钧, 等. 优良八仙花品种(Hydrangea serrata‘Preziosa’)的离体培养与快速繁殖[J]. 上海农业学报, 2010, 26(1): 38-41.
[14]	KHAING M T, JUNG H J, HAN T H. Effect of plant growth regulators on regeneration and proliferation of Hydrangea macrophylla cultivars[J]. Flower Research Journal, 2018, 26(3): 84-89.
[15]	孙晓波, 苏家乐, 陈双双, 等. 大花绣球‘无尽夏’组培苗叶片再生植株的研究[J]. 中国农学通报, 2020, 36(16): 67-72.
[16]	臧萌. 流苏相思优良无性系离体培养与快速繁殖技术研究[D]. 福州: 福建农林大学, 2009.
[17]	李杨丽. 八仙花离体快繁及盆栽生产相关技术研究[D]. 武汉: 华中农业大学, 2010.
[18]	秦紫艺, 陈双双, 王华娣, 等. 绣球属植物组织培养研究进展[J]. 江苏农业科学, 2021, 49(19): 45-50.
[19]	蒋梦烟. 绣球品种‘蓝尼康’快繁技术研究[D]. 长沙: 中南林业科技大学, 2017.
[20]	张瑞姿. 植物生长调节剂在观赏植物组织培养中的应用[J]. 山西林业, 2012(5): 39-40.
[21]	王红梅, 陈婷婷, 刘春风, 等. 圆锥绣球 “粉色精灵” 组培快繁技术研究[J]. 种子, 2022, 41(4): 130-137, 封3.
[22]	余孟娟, 孙婷婷, 董诚明, 等. 半夏组培苗再生途径优选及驯化移栽[J]. 植物生理学报, 2022, 58(1): 214-222.
[23]	房超, 李金鑫, 余鹏, 等. 基于泥炭土、蛭石和珍珠岩的混配对赤泥土壤化改良的试验研究[J]. 山东化工, 2017, 46(6): 146-148.

处理	基本培养基	6-BA/(mg·L^-1)	IBA/(mg·L^-1)
1	MS	1.0	0
2	MS	1.5	0.1
3	MS	2.0	0.2
4	MS	2.5	0.3
5	1/2 MS	1.0	0.1
6	1/2 MS	1.5	0
7	1/2 MS	2.0	0.3
8	1/2 MS	2.5	0.2
9	B5	1.0	0.2
10	B5	1.5	0.3
11	B5	2.0	0
12	B5	2.5	0.1
13	1/2 B5	1.0	0.3
14	1/2 B5	1.5	0.2
15	1/2 B5	2.0	0.1
16	1/2 B5	2.5	0

处理	基本培养基	6-BA/(mg·L^-1)	IBA/(mg·L^-1)
1	MS	1.0	0
2	MS	1.5	0.1
3	MS	2.0	0.2
4	MS	2.5	0.3
5	1/2 MS	1.0	0.1
6	1/2 MS	1.5	0
7	1/2 MS	2.0	0.3
8	1/2 MS	2.5	0.2
9	B5	1.0	0.2
10	B5	1.5	0.3
11	B5	2.0	0
12	B5	2.5	0.1
13	1/2 B5	1.0	0.3
14	1/2 B5	1.5	0.2
15	1/2 B5	2.0	0.1
16	1/2 B5	2.5	0

处理	基本培养基	6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	萌芽率/ %
1	MS	0.5	0	47.0±2.1 d
2	MS	1.0	0.1	57.0±1.5 c
3	MS	1.5	0.2	66.0±1.6 b
4	MS	2.0	0.3	57.0±1.5 c
5	1/2 MS	0.5	0.1	44.0±2.2 d
6	1/2 MS	1.0	0	55.0±1.7 c
7	1/2 MS	1.5	0.3	58.0±2.0 c
8	1/2 MS	2.0	0.2	66.0±1.6 b
9	B5	0.5	0.2	29.0±1.0 e
10	B5	1.0	0.3	32.0±1.3 e
11	B5	1.5	0	70.0±2.1 ab
12	B5	2.0	0.1	66.0±1.6 b
13	1/2 B5	0.5	0.3	47.0±2.1 d
14	1/2 B5	1.0	0.2	33.0±2.1 e
15	1/2 B5	1.5	0.1	60.0±1.8 c
16	1/2 B5	2.0	0	74.0±2.2 a

处理	基本培养基	6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	萌芽率/ %
1	MS	0.5	0	47.0±2.1 d
2	MS	1.0	0.1	57.0±1.5 c
3	MS	1.5	0.2	66.0±1.6 b
4	MS	2.0	0.3	57.0±1.5 c
5	1/2 MS	0.5	0.1	44.0±2.2 d
6	1/2 MS	1.0	0	55.0±1.7 c
7	1/2 MS	1.5	0.3	58.0±2.0 c
8	1/2 MS	2.0	0.2	66.0±1.6 b
9	B5	0.5	0.2	29.0±1.0 e
10	B5	1.0	0.3	32.0±1.3 e
11	B5	1.5	0	70.0±2.1 ab
12	B5	2.0	0.1	66.0±1.6 b
13	1/2 B5	0.5	0.3	47.0±2.1 d
14	1/2 B5	1.0	0.2	33.0±2.1 e
15	1/2 B5	1.5	0.1	60.0±1.8 c
16	1/2 B5	2.0	0	74.0±2.2 a

处理	基本培养基	6-BA/ (mg·L^-1)	NAA/ (mg·L^-1)	植株生长状态
1	MS	1.0	0	植株矮小,叶片发黄,无愈伤
2	MS	1.5	0.1	植株矮小,叶片发黄,无愈伤
3	MS	2.0	0.2	植株矮小,叶片发黄,无愈伤
4	MS	2.5	0.3	植株矮小,叶色正常,无愈伤
5	1/2 MS	1.0	0.1	植株矮小,叶片发黄,有愈伤
6	1/2 MS	1.5	0	植株正常,叶色正常,有愈伤
7	1/2 MS	2.0	0.3	植株矮小,叶片发黄,无愈伤
8	1/2 MS	2.5	0.2	植株正常,叶色正常,有愈伤
9	B5	1.0	0.2	植株矮小,叶色正常,无愈伤
10	B5	1.5	0.3	植株正常,叶色发黄,有愈伤
11	B5	2.0	0	植株正常,叶色正常,少量愈伤
12	B5	2.5	0.1	植株矮小,叶色正常,有愈伤
13	1/2 B5	1.0	0.3	植株正常,叶色正常,有愈伤
14	1/2 B5	1.5	0.2	植株正常,叶色正常,有愈伤
15	1/2 B5	2.0	0.1	植株正常,叶色正常,有愈伤
16	1/2 B5	2.5	0	植株健壮,叶色正常,无愈伤