Effects of plant hormones and lactic acid bacteria on the growth of Ginkgo biloba L. leaves

doi:10.16178/j.issn.0528-9017.20240612

Abstract

Abstract:

In order to explore the biological fertilization formula for growth regulation of Ginkgo biloba leaves, gibberellin, cytokinin and trace elements were applied with lactic acid bacteria solution to study the effects of these two plant hormones, trace elements and microbial fertilizers on the growth of Ginkgo biloba leaves. The results showed that 4% boron and 4% gibberellin treatment had the best effect on delaying the leaf withering, increasing the length and width of leaves, and reducing the fruiting yield of Ginkgo biloba, followed by 8% manganese and 4% gibberellin treatments. After the treatment of trace elements of 4% boron + 8% manganese and 4% gibberellin, the defoliation time of Ginkgo biloba was delayed for more than a week, and the fruiting yield of Ginkgo biloba was reduced by 24.96%. In addition, it was found that the combination of 4% boron + 8% manganese, 4% gibberellin and lactic acid bacteria solution and the use of hydroxy propyl methyl cellulose (HPMC) membrane for pollination isolation had the most significant effect on the growth of Ginkgo biloba leaves.

Key words: Ginkgo biloba L., plant hormones, microelements, lactic acid bacteria

CLC Number:

S792.95

CHEN Hai, REN Ruochen, ZHU Pei, ZHU Lili, XU Zhenlan, WANG Qi, ZHANG Yuxiang, JIANG Min. Effects of plant hormones and lactic acid bacteria on the growth of Ginkgo biloba L. leaves[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(12): 2939-2943.

Figures/Tables 9

References 17

[1]	BARDGETT R D, VAN DER PUTTEN W H. Belowground biodiversity and ecosystem functioning[J]. Nature, 2014, 515(7528): 505-511.
[2]	LIU Z, GUO Q, FENG Z Y, et al. Long-term organic fertilization improves the productivity of kiwifruit (Actinidia chinensis Planch.) through increasing rhizosphere microbial diversity and network complexity[J]. Applied Soil Ecology, 2020, 147: 103426.
[3]	LIU J A, SHU A P, SONG W F, et al. Long-term organic fertilizer substitution increases rice yield by improving soil properties and regulating soil bacteria[J]. Geoderma, 2021, 404: 115287.
[4]	FAN K K, DELGADO-BAQUERIZO M, GUO X S, et al. Biodiversity of key-stone phylotypes determines crop production in a 4-decade fertilization experiment[J]. The ISME Journal, 2021, 15(2): 550-561.
[5]	WU W Q, DU K, KANG X Y, et al. The diverse roles of cytokinins in regulating leaf development[J]. Horticulture Research, 2021, 8(1): 118.
[6]	RITONGA F N, ZHOU D D, ZHANG Y H, et al. The roles of gibberellins in regulating leaf development[J]. Plants, 2023, 12(6): 1243.
[7]	KAUR H, KAUR H, KAUR H, et al. The beneficial roles of trace and ultratrace elements in plants[J]. Plant Growth Regulation, 2023, 100(2): 219-236.
[8]	SINGH B, KAUR P, GOPICHAND, et al. Biology and chemistry of Ginkgo biloba[J]. Fitoterapia, 2008, 79(6): 401-418.
[9]	吴建伟, 林强, 郑剑钧, 等. 银杏叶提取物注射液辅助治疗老年缺血性脑血管病的效果及对患者血液流变学、凝血功能的影响[J]. 中国医学创新, 2023, 20(20): 11-15.
[10]	付佳乐, 耿直, 郭亚敏, 等. 银杏叶总黄酮绿色提取工艺及抗氧化活性研究[J]. 化学与生物工程, 2024, 41(1): 40-45.
[11]	杜少严, 尹硕, 王意浓, 等. 银杏叶的药用与保健价值及其应用[J]. 中国食物与营养, 2020, 26(6): 59-62.
[12]	SABLOWSKI R, CARNIER DORNELAS M. Interplay between cell growth and cell cycle in plants[J]. Journal of Experimental Botany, 2014, 65(10): 2703-2714.
[13]	杨小芹, 窦凯. 植物激素在果树生长与环境适应中的作用[J]. 果树资源学报, 2024, 5(3): 112-114.
[14]	殷齐慧, 王胜华, 王茂锁, 等. 5种不同的植物激素对白花前胡种子萌发的影响[J]. 中南药学, 2024, 22(5): 1245-1249.
[15]	YANG W L, GONG T, WANG J W, et al. Effects of compound microbial fertilizer on soil characteristics and yield of wheat (Triticum aestivum L.)[J]. Journal of Soil Science and Plant Nutrition, 2020, 20(4): 2740-2748.
[16]	侯景清, 王旭, 陈玉海, 等. 乳酸菌复合制剂在盐碱地培肥及促进蔬菜生长效果的研究[J]. 内蒙古农业大学学报(自然科学版), 2020, 41(2): 37-43.
[17]	DEWI A K, RAHAYU S, DWIMAHYANI I, et al. The combination of irradiation biofertilizer of rhizosphere microbes consortium inoculant (IMR) and inorganic fertilizer on the growth of kale in a floating raft hydroponic system[J]. IOP Conference Series: Earth and Environmental Science, 2021, 924(1): 012007.

胸径/ cm	微量元素施用量/ (mL·cm^-1)	尿素施用量/ (kg·株^-1)	过磷酸钙施用量/ (kg·株^-1)	氯化钾施用量/ (kg·株^-1)
15	50	6	2	2
>15~30	100	9	3	3
>30	150	12	4	4

胸径/ cm	微量元素施用量/ (mL·cm^-1)	尿素施用量/ (kg·株^-1)	过磷酸钙施用量/ (kg·株^-1)	氯化钾施用量/ (kg·株^-1)
15	50	6	2	2
>15~30	100	9	3	3
>30	150	12	4	4

处理	化肥配施比例/%			微量元素/植物激素
处理	尿素	过磷酸钙	氯化钾	微量元素/植物激素
CK	30	10	10	0
T1	30	10	10	0.8%钼+3.5%细胞分裂素
T2	30	10	10	4%硼+3.5%细胞分裂素
T3	30	10	10	4%硼+4%赤霉素
T4	30	10	10	8%锰+4%赤霉素
T5	30	10	10	4%锌+4%赤霉素
T6	30	10	10	0.8%钼+4%赤霉素
T7	30	10	10	8%锰+3.5%细胞分裂素
T8	30	10	10	4%锌+3.5%细胞分裂素
T9	30	10	10	0.8%钼+4%硼+3.5%细胞分裂素
T10	30	10	10	0.8%钼+8%锰+3.5%细胞分裂素
T11	30	10	10	0.8%钼+4%锌+3.5%细胞分裂素
T12	30	10	10	4%硼+8%锰+3.5%细胞分裂素
T13	30	10	10	4%硼+4%锌+3.5%细胞分裂素
T14	30	10	10	8%锰+4%锌+3.5%细胞分裂素
T15	30	10	10	4%硼+8%锰+4%赤霉素
T16	30	10	10	4%硼+4%锌+4%赤霉素
T17	30	10	10	4%硼+0.8%钼+4%赤霉素
T18	30	10	10	8%锰+4%锌+4%赤霉素
T19	30	10	10	8%锰+0.8%钼+4%赤霉素
T20	30	10	10	4%锌+0.8%钼+4%赤霉素
T21	0	0	0	0
T22	30	10	10	乳酸菌灌根
T23	30	10	10	注干剂(自制)+乳酸菌灌根
T24	30	10	10	注干剂(自制)+乳酸菌灌根+HPMC膜授粉隔离

处理	化肥配施比例/%			微量元素/植物激素
处理	尿素	过磷酸钙	氯化钾	微量元素/植物激素
CK	30	10	10	0
T1	30	10	10	0.8%钼+3.5%细胞分裂素
T2	30	10	10	4%硼+3.5%细胞分裂素
T3	30	10	10	4%硼+4%赤霉素
T4	30	10	10	8%锰+4%赤霉素
T5	30	10	10	4%锌+4%赤霉素
T6	30	10	10	0.8%钼+4%赤霉素
T7	30	10	10	8%锰+3.5%细胞分裂素
T8	30	10	10	4%锌+3.5%细胞分裂素
T9	30	10	10	0.8%钼+4%硼+3.5%细胞分裂素
T10	30	10	10	0.8%钼+8%锰+3.5%细胞分裂素
T11	30	10	10	0.8%钼+4%锌+3.5%细胞分裂素
T12	30	10	10	4%硼+8%锰+3.5%细胞分裂素
T13	30	10	10	4%硼+4%锌+3.5%细胞分裂素
T14	30	10	10	8%锰+4%锌+3.5%细胞分裂素
T15	30	10	10	4%硼+8%锰+4%赤霉素
T16	30	10	10	4%硼+4%锌+4%赤霉素
T17	30	10	10	4%硼+0.8%钼+4%赤霉素
T18	30	10	10	8%锰+4%锌+4%赤霉素
T19	30	10	10	8%锰+0.8%钼+4%赤霉素
T20	30	10	10	4%锌+0.8%钼+4%赤霉素
T21	0	0	0	0
T22	30	10	10	乳酸菌灌根
T23	30	10	10	注干剂(自制)+乳酸菌灌根
T24	30	10	10	注干剂(自制)+乳酸菌灌根+HPMC膜授粉隔离