Effects of different gibberellin concentrations and stratification temperatures on seed germination of Polygonatum sibiricum

doi:10.16178/j.issn.0528-9017.20241003

Abstract

Abstract:

This study explored the effects of different gibberellin treatments and stratification temperatures on seed germination of Polygonatum sibiricum, aiming to provide a technical solution for the large-scale and factory-based seedling planting of P. sibiricum. The results showed that treatment with 100 mg·L^-1 gibberellin could effectively broke seed dormancy, and the germination rate was as high as 61.75%. In addition, the treatment effect is the best when gibberellin is combined with low-temperature sand storage. Under the conditions of 15 ℃ and 100 mg·L^-1 gibberellin, the germination rate of seeds reached the maximum value. After 56 days of sand storage, the seeds were transferred to peat soil for cultivation for 10 days, and the germination rate reached 86.51%. Furthermore, with the extension of sand storage time, the germination time of seeds was significantly shortened, and the concentration of abscisic acid (ABA) in seeds showed a downward trend, which also indirectly indicated that the germination rate was negatively correlated with the ABA content. This study provides optimized conditions and technical support for the preservation and germination of Polygonatum sibiricum seeds.

Key words: Polygonatum sibiricum, sand storage, seed germination

CLC Number:

S567

XI Gangjun, YANG Hetong, SHI Jun, GAO Daxiang, JIA Jun. Effects of different gibberellin concentrations and stratification temperatures on seed germination of Polygonatum sibiricum[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(7): 1621-1626.

Figures/Tables 6

References 17

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赤霉素浓度/ (mg·L^-1)	种子发芽率
赤霉素浓度/ (mg·L^-1)	14 d	21 d	28 d	35 d	42 d
CK	0	0	15.26	22.45	32.23
50	12.56	26.32	34.65	48.58	53.86
100	22.68	33.57	37.86	55.38	61.75
200	9.85	23.58	30.76	42.67	48.56

赤霉素浓度/ (mg·L^-1)	种子发芽率
赤霉素浓度/ (mg·L^-1)	14 d	21 d	28 d	35 d	42 d
CK	0	0	15.26	22.45	32.23
50	12.56	26.32	34.65	48.58	53.86
100	22.68	33.57	37.86	55.38	61.75
200	9.85	23.58	30.76	42.67	48.56

贮藏温度/ ℃	赤霉素浓度/ (mg·L^-1)	发芽势/ %	发芽率/ %
4	0	35.21±2.65 cd	43.43±2.12 c
	50	39.34±3.42 d	49.14±2.57 d
	100	54.27±3.56 f	67.01±4.23 f
	200	42.55±2.89 de	52.00±2.16 de
15	0	20.53±3.50 ab	40.10±3.27 c
	50	55.84±4.78 f	68.42±3.65 f
	100	67.53±3.89 g	83.51±4.27 g
	200	43.28±3.56 e	55.17±2.56 e
25	0	19.50±2.78 ab	33.33±3.45 b
	50	20.6±3.50 ab	28.21±2.78 ab
	100	33.9±2.64 c	53.33±3.36 e
	200	16.6±2.83 a	24.59±3.89 a

贮藏温度/ ℃	赤霉素浓度/ (mg·L^-1)	发芽势/ %	发芽率/ %
4	0	35.21±2.65 cd	43.43±2.12 c
	50	39.34±3.42 d	49.14±2.57 d
	100	54.27±3.56 f	67.01±4.23 f
	200	42.55±2.89 de	52.00±2.16 de
15	0	20.53±3.50 ab	40.10±3.27 c
	50	55.84±4.78 f	68.42±3.65 f
	100	67.53±3.89 g	83.51±4.27 g
	200	43.28±3.56 e	55.17±2.56 e
25	0	19.50±2.78 ab	33.33±3.45 b
	50	20.6±3.50 ab	28.21±2.78 ab
	100	33.9±2.64 c	53.33±3.36 e
	200	16.6±2.83 a	24.59±3.89 a

沙藏层积时长/d	初始萌发时长/d	发芽势/ %	发芽率/ %
28	22	5.35±1.26 a	42.53±3.36 a
42	18	34.28±4.18 b	68.75±5.27 b
56	10	72.38±3.27 c	86.51±3.68 c