Effect of soaking seeds in gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide on the germination of calabash gourd seeds and the growth of seedlings

doi:10.16178/j.issn.0528-9017.20240395

Abstract

Abstract:

To determine the effect of different plant growth regulators on seed germination and seedling growth of calabash gourd, the germination rate of calabash gourd seeds treated with five plant growth regulators was measured using a double-layer filter paper cultivation method under indoor conditions. Under plastic greenhouse production conditions, pot experiments were conducted to determine the effects of soaking seeds in different concentrations of gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide, gibberellic acid, water for 16 h on seed germination rate, germination potential, germination index and vitality index of calabash gourd seeds. The aboveground fresh weight, root weight, plant height, root length and stem base width of the seedlings were also measured. The results showed that 0.4, 4.0 mg·L^-1 gibberellic acid and 1.0 mg·L^-1 gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide could promote the germination of calabash gourd seeds under indoor conditions. Under plastic greenhouse production conditions, 0.5 and 1.0 mg·L^-1 gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide could improve the seed germination rate, germination index and vitality index of calabash gourd seedlings. In addition, 0.5 and 1.0 mg·L^-1 gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide promoted seed coat shedding and helped the true leaves to stretch in time. 0.5 and 1.0 mg·L^-1 gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide promoted the growth of calabash gourd seedlings, and were significantly superior to other methods in aboveground fresh weight, root weight, plant height, root length and stem base width. In summary, soaking seeds in 0.5 and 1.0 mg·L^-1 gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide for 16 h before sowing can promote the germination of calabash gourd seeds and the growth of seedlings, which is recommended to promote the application.

Key words: plant growth regulator, calabash gourd seed, germination rate, seedling growth

CLC Number:

S642

XIANG Binghan, HE Jie, CAI Xinyi, PAN Sufeng, YAN Chengjin, GAO Liuxiao, CHEN Jiajia, YU Yangbo, ZHANG Zhen, YU Haibing. Effect of soaking seeds in gibberellic acid·indol-3-ylacetic acid·28-epihomobrassinolide on the germination of calabash gourd seeds and the growth of seedlings[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(8): 1871-1876.

Figures/Tables 8

References 15

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处理方式	推荐使用浓度/ (mg·L^-1)	试验浓度/ (mg·L^-1)
清水	0	0	0	0
14-羟基芸苔素甾醇	4	0.4	4.0	40.0
28-高芸苔素内酯	40	4.0	40.0	400.0
24-表高芸苔素内酯	40	4.0	40.0	400.0
赤霉酸	4	0.4	4.0	40.0
28-表高芸·赤·吲乙	4	0.1	1.0	10.0

处理方式	推荐使用浓度/ (mg·L^-1)	试验浓度/ (mg·L^-1)
清水	0	0	0	0
14-羟基芸苔素甾醇	4	0.4	4.0	40.0
28-高芸苔素内酯	40	4.0	40.0	400.0
24-表高芸苔素内酯	40	4.0	40.0	400.0
赤霉酸	4	0.4	4.0	40.0
28-表高芸·赤·吲乙	4	0.1	1.0	10.0

处理方式	使用剂量/(mg·L^-1)
清水	0
磕种	0
28-表高芸·赤·吲乙	0.5
	1.0
	2.0
赤霉酸	4.0

处理方式	使用剂量/(mg·L^-1)
清水	0
磕种	0
28-表高芸·赤·吲乙	0.5
	1.0
	2.0
赤霉酸	4.0

处理方式	浓度/ (mg·L^-1)	最终萌发率/%	标准误	差异显著性
清水	0	57.78	5.879	b
14-羟基芸苔素甾醇	0.4	64.44	4.443	b
	4.0	66.67	0	b
	40.0	51.11	2.220	b
28-高芸苔素内酯	4.0	40.00	11.547	c
	40.0	64.44	8.013	b
	400.0	13.33	3.848	d
24-表高芸苔素内酯	4.0	55.56	2.223	b
	40.0	31.11	5.879	c
	400.0	35.56	4.443	c
赤霉酸	0.4	75.56	4.443	a
	4.0	71.11	4.443	a
	40.0	31.11	4.443	c
28-表高芸·赤·吲乙	0.1	57.78	2.223	b
	1.0	82.22	2.223	a
	10.0	40.00	0	c