Identification and pathogenicity of pathogens of garlic（Allium sativum L.） root rot

doi:10.16178/j.issn.0528-9017.20260357

Abstract

Abstract:

From garlic root rot samples collected in Shanghe County，Shandong Province，a total of 20 isolates were obtained. These isolates were identified using a combination of morphological and molecular approaches，and their pathogenicity to garlic seedlings was assessed through soil inoculation. The results showed that 17 isolates were identified as Fusarium oxysporum，and three isolates as F. ipomoeae. Both Fusarium species exhibited pathogenicity to garlic seedlings，confirming them as causal agents of garlic root rot. Among them，F. oxysporum displayed stronger pathogenicity，with average diseased plant rate and disease index reaching 95.24% and 59.59，respectively；whereas F. ipomoeae showed weaker pathogenicity，with average diseased plant rate and disease index of 66.67% and 20.32，respectively. Notably，the identification of F. ipomoeae as a pathogen of garlic root rot represented the first report of its kind，providing a theoretical basis for disease-resistant breeding and field control of garlic root rot.

Key words: garlic, root rot, pathogen identification, Fusarium, pathogenicity

CLC Number:

S436.33

GUO Lianjun, WANG Yajing, LI Teng, CUI Kai, DONG Zhan. Identification and pathogenicity of pathogens of garlic（Allium sativum L.） root rot[J]. Journal of Zhejiang Agricultural Sciences, 2026, 67(9): 2235-2239.

Figures/Tables 4

Fig. 1 Colony morphology and microscopic characteristics of F. oxysporum and F. ipomoeae

Fig.2 Systematic phylogenetic tree constructed using the maximum likelihood method based on the TEF-1α gene sequence

Fig.3 Pathogenicity assays of the representative isolates of F. oxysporum and F. ipomoeae

Table 1 Disease incidence and disease index on garlic seedlings inoculated with representative isolates

镰刀菌种类	病株率/%			病情指数
镰刀菌种类	最小值	最大值	平均值	最小值	最大值	平均值
尖孢镰刀菌	66.67	100.00	95.24	15.24	79.05	59.59
番薯镰刀菌	46.67	80.00	66.67	12.38	27.62	20.32

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