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11 September 2026, Volume 67 Issue 9 Previous Issue   

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Analysis of current status and quality improvement strategies of seed quality in Zhejiang Province HAO Wanjun, WU Zaogui, HE Weijun 2026, 67 (9):  2105-2108.  DOI: 10.16178/j.issn.0528-9017.20260494 Abstract ( 58 )   HTML ( 2 )   PDF (401KB) ( 177 )   Seed is the “chip” of agriculture，and its quality is directly related to national food security and agricultural sustainable development. To explore the current status of seed quality in Zhejiang Province，this paper systematically analyzed the inspection data of seed samples from the seed quality monitoring campaigns in Zhejiang Province during 2015-2024. The results showed that the overall seed quality in Zhejiang Province could basically meet agricultural production needs；however，the seed quality still lacked competitiveness，with prominent issues such as non-standard seed packaging，insufficient enterprise quality management capabilities，weak compliance awareness，and inadequate attention to seed vigor and seed health. In response to the above problems，this paper proposed countermeasures and suggestions including raising ideological awareness，improving quality standard systems，comprehensively implementing seed certification，strengthening supervision and random inspection of seed quality，and enhancing publicity and guidance. Figures and Tables | References | Related Articles | Metrics

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High-efficiency cultivation techniques for early rice variety Zhongzu 18 in the coastal region of Taizhou CHEN Jun, XU Qiangqiang, YU Pengcheng, DING Yangdong, BAO Zuda 2026, 67 (9):  2109-2112.  DOI: 10.16178/j.issn.0528-9017.20250213 Abstract ( 58 )   HTML ( 4 )   PDF (334KB) ( 153 )   To investigate the cultivation techniques of the high-quality early-maturing rice variety Zhongzu 18 in the coastal area of Taizhou，comparative experiments were conducted under different cultivation methods，planting densities，and nitrogen fertilizer levels，with the aim of summarizing a high-yield cultivation technical scheme for Zhongzu 18 in this region. The results showed that Zhongzu 18 had moderate tillering ability and small panicle size，so direct sowing was recommended to increase effective panicles. With a seed rate of 5-6 kg per 667 m2 and a pure nitrogen application rate of 12 kg per 667 m2，ideal effective panicle numbers and panicle size could be achieved，and the yield potential was significantly improved. Figures and Tables | References | Related Articles | Metrics

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Effects of different seedling raising agents on seedling vigor and stress resistance of ratoon rice under early spring low-temperature stress in southern Henan rice region ZHAO Haiying, LI Gexing, GU Mengxuan, FENG Daqing, YU Guilong, ZHANG Qiang, LIU Xiangchen, FU Qiang, LIU Yali 2026, 67 (9):  2113-2118.  DOI: 10.16178/j.issn.0528-9017.20260373 Abstract ( 50 )   HTML ( 2 )   PDF (465KB) ( 147 )   To screen high-efficiency seedling-raising agents suitable for early-spring seedling raising of ratoon rice in the southern Henan rice region，and alleviate low-temperature stress caused by late spring cold and the damage of sheath blight，this experiment took Taoyou Xiangzhan， the main cultivar of ratoon rice in southern Henan， as the test material. A low-temperature stress environment was simulated in an artificial climate chamber，with five treatments（CK，pure soil；Z，Longqi brand seedling-raising agent；Y，self-developed seedling-raising agent；X，Dr. Xu brand seedling-strengthening adjuvant；S，Chengbiao brand sulfur）set up. The regulatory effects of different treatments on seedling quality and physiological and biochemical indices were compared，and the stress resistance mechanism and application value of the self-developed formula were clarified. The results showed that under low temperature stress，compared with CK，all treatments could improve the seedling quality，among which the Y and Z treatments had better effects. The stem base width of the seedlings in treatments Y and Z significantly （p<0.05） increased by 41.2% compared with CK. The fresh weight of stems and roots in treatment Y significantly increased by 148.1% and 41.2% compared with CK. The total root length，total root surface area，and total root volume significantly increased by 67.6%，55.2%，and 100.0%， respectively， compared with CK. The incidence of seedling blight was reduced by 58.67 percentage points compared with CK. Treatment Y activated the antioxidant system：the activities of peroxidase（POD），catalase（CAT）and superoxide dismutase（SOD） significantly increased by 45.56%，58.48%，35.45% compared with CK，respectively；the malondialdehyde（MDA）content significantly decreased by 28.6%，the proline and soluble sugar content were increased by 61.08%，72.88%， respectively， compared with CK，effectively maintaining cell membrane integrity and osmotic adjustment capacity. The results showed that the self-developed seedling raising agent Y significantly enhanced the low-temperature tolerance and blight resistance of ratoon rice seedlings in southern Henan，providing theoretical basis and technical support for safe early-spring seedling raising of regional ratoon rice. Figures and Tables | References | Related Articles | Metrics

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Effect of “one-basal-one-topdressing” fertilization mode on rice yield and nutrient uptake and utilization LUO Chunhong, SHI Yong, LYU Zhiwei, JIN Meijuan, ZHANG Yanhui, WANG Haihou 2026, 67 (9):  2119-2123.  DOI: 10.16178/j.issn.0528-9017.20250261 Abstract ( 52 )   HTML ( 0 )   PDF (459KB) ( 161 )   To investigate the responses of yield and nutrient use efficiency of different rice varieties to the “one-basal-one-topdressing” fertilization mode，field experiments were conducted with two rice varieties，Huruanyu 1 and Sujing 4699. Two treatments were established：conventional fertilization mode（T1）with broadcasting of basal fertilizer，tillering fertilizer，and panicle fertilizer，and the “one-basal-one-topdressing” mode（T2）with side-deep basal fertilization and broadcasting of panicle fertilizer. The yield，yield components，panicle setting rate，and nutrient use efficiency were compared between treatments. The results showed that under T2，the yields per 667 m2 of Huruanyu 1 and Sujing 4699 were 623.5 and 611.8 kg，respectively，with no significant difference from T1. The panicle setting rate under T2 were 76.5% for Huruanyu 1 and 70.1% for Sujing 4699，which were significantly increased by 6.6 and 6.9 percentage points compared with T1. Regarding fertilizer use efficiency，T2 significantly increased the uptake of nitrogen，phosphorus，and potassium，as well as grain production efficiency and dry matter production efficiency for both varieties. Specifically，the partial factor productivity of nitrogen fertilizer for Huruanyu 1 and Sujing 4699 under T2 increased by 40.7% and 20.3%，respectively，compared with T1；that of phosphorus fertilizer increased by 307.3% and 249.3%，respectively，and that of potassium fertilizer increased by 103.8% and 74.6%，respectively. Variety comparison indicated that Huruanyu 1 exhibited generally higher nutrient use efficiency than Sujing 4699 under T2. In conclusion，the “one-basal-one-topdressing” mode with side-deep basal fertilization and broadcasting of panicle fertilizer can achieve both stable rice yield and improved nutrient uptake and utilization even under reduced N，P2O5，and K2O application rates. This mode is more suitable for high-efficiency production of Huruanyu 1. For Sujing 4699，it is suggested to appropriately reduce the proportion of basal nutrients to control unproductive tillers and increase the amount of panicle fertilizer to promote the formation of productive panicles，thereby further improving its yield stability and nutrient use efficiency. Figures and Tables | References | Related Articles | Metrics

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Screening test of herbicide-resistant rice varieties in Huaibei region of Jiangsu Province in 2024 WANG Wu, WANG Jingjing, ZHANG Peng, ZHANG Yanbing, WANG Kangkang, MAO Xiaoye 2026, 67 (9):  2124-2127.  DOI: 10.16178/j.issn.0528-9017.20250573 Abstract ( 47 )   HTML ( 1 )   PDF (385KB) ( 163 )   To screen new medium-maturing and medium-japonica herbicide-resistant rice varieties suitable for planting in Huaibei region of Jiangsu Province，this study conducted a field experiment at the experimental farm of Dongxin Branch of Jiangsu Agricultural Reclamation Development Co.，Ltd. in 2024. A comprehensive evaluation was carried out on the high-yielding potential，main agronomic traits，and field resistance of 17 tested varieties（including the control Huajing 5）. The results showed that Lianjing 166 had the highest yield，moderate growth duration，and good lodging resistance，indicating great potential for popularization and application. Runnong 17 ranked second in yield，with lower plant height，good lodging resistance，and shorter growth duration，offering certain flexibility in crop rotation arrangement. Runnong 802 had the lowest yield and poor lodging resistance，and was recommended to be eliminated or further tested. Future efforts should continue to focus on the stability and adaptability of herbicide-resistant rice varieties. Figures and Tables | References | Related Articles | Metrics

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Comparative trials and comprehensive evaluation of 15 new wheat varieties in northern Zhejiang Province AO Li, FAN Min, HUA Wei, WANG Weidong, PENG Guifu, ZHU Jinghuan 2026, 67 (9):  2128-2132.  DOI: 10.16178/j.issn.0528-9017.20250791 Abstract ( 55 )   HTML ( 1 )   PDF (406KB) ( 144 )   To evaluate the growth characteristics of high-yielding wheat varieties in northern Zhejiang，refine supporting cultivation techniques，and provide technical support for the high-quality development of the local wheat industry and the widespread adoption of new varieties，this study used Yangmai 28 as a control to investigate the growth period，Fusarium head blight resistance，lodging resistance，yield，and key economic traits of 15 new wheat varieties cultivated in Changxing，Zhejiang. Results showed significant variations among the tested varieties in terms of growth period，Fusarium head blight resistance，lodging resistance，and yield. Among the 15 varieties，Ningmai 24，Yangmai 39，Zhehua No.1，Zhefengmai 11，Nongmai 77，and Nongmai 99 all achieved yields exceeding 400 kg per 667 m2. Considering Fusarium head blight resistance，lodging resistance，and cold tolerance comprehensively，Zhefengmai 11 demonstrated the best performance with a Fusarium head blight disease index of 7.0，lodging resistance rating of 3，and cold tolerance grade of 1；followed by Yangmai 39，Nongmai 99，and Zhehua No.1. In conclusion，these varieties showed excellent potential for widespread adoption in northern Zhejiang. Figures and Tables | References | Related Articles | Metrics

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Effects of seeding date and harvest date on the quality of fresh-eating Huangjinxiaoyumi NIU Liying, CAI Chenxiang, ZHAO Wenming, FENG Jialin, LIU Ningyue, WU Haihong, NIE Meimei, LIU Tianxing, LI Dajing, CHEN Yanping 2026, 67 (9):  2133-2139.  DOI: 10.16178/j.issn.0528-9017.20250329 Abstract ( 44 )   HTML ( 0 )   PDF (688KB) ( 143 )   Huangjinxiaoyumi is a national geographical indication agricultural product，belonging to fresh-eating waxy corn，and is characterized by its small ear size and sweet，glutinous taste. In this study，two fresh-eating waxy corn cultivars，Suyunuo 5 and Jinnuo 262，were used as controls to compare the ear shape，kernel traits，and kernel sensory quality of Huangjinxiaoyumi harvested at seven dates across two seeding dates（spring and summer）. The results showed that at 17-20 days after pollination，Huangjinxiaoyumi had ear length of 10.5-11.8 cm，ear diameter of 2.4-3.0 cm，and fresh hundred-kernel weight of 14.5-16.5 g，all of which were lower than those of Suyunuo 5 and Jinnuo 262. In addition，Huangjinxiaoyumi exhibited lower kernel moisture content，higher hardness，higher soluble solids content，and stronger aroma，while its sweetness and tenderness were greatly influenced by seeding date and harvest date. Huangjinxiaoyumi also featured a short development period，rapid changes in kernel quality，and high sensitivity to climatic conditions. Therefore，appropriate sowing date and timely harvest are important measures to improve the quality stability of Huangjinxiaoyumi and ensure the healthy development of its industry. Figures and Tables | References | Related Articles | Metrics

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Integration and application of high-yield and high-efficiency cultivation technique for feed barley ZHANG Liangcheng, XU Jun, HUAI Yan, MENG Zhonglei, ZHANG Yao, WANG Junmei, YUE Wenhao, LIU Lei, LI Yong, CAI Kangfeng 2026, 67 (9):  2140-2142.  DOI: 10.16178/j.issn.0528-9017.20250582 Abstract ( 43 )   HTML ( 0 )   PDF (389KB) ( 154 )   The high-yield and high-efficiency cultivation technique for feed barley was integrated in the context of climate conditions and production experience in Zhejiang Province. The technique covers aspects such as field pretreatment，variety selection，seed dressing，timely sowing，efficient fertilization，chemical regulation，pest，disease and weed control，stress tolerance improvement，as well as timely harvest. The technique was applied in Pinghu，Zhejiang from 2024 to 2025，achieving an average yield of 9 563.5 kg·hm-2，which represented a 33.9% increase over the provincial high-yield record for barley under conventional cultivation practices in Zhejiang. Efforts should be made to promote and demonstrate this technique to underpin the large-scale yield increase of feed barley in Zhejiang Province. Figures and Tables | References | Related Articles | Metrics

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Effects of organic fertilizer formulations on oil content，yield and quality of flue-cured tobacco leaves ZHU Zhizhong, GE Zixuan, DUAN Weidong, WANG Xinzhong, GUO Fangyang, WU Zhaohui, XU Wenzheng 2026, 67 (9):  2143-2148.  DOI: 10.16178/j.issn.0528-9017.20250547 Abstract ( 60 )   HTML ( 0 )   PDF (493KB) ( 153 )   To investigate the effects of different formulations of organic fertilizer on oil content，yield and quality of flue-cured tobacco leaves，a field experiment was conducted in the Xuchang tobacco-growing area. Four treatments were set up：local farmers' conventional fertilization as the control（CK），and three organic fertilizer treatments with different formulations，designated as Formula 1，Formula 2，and Formula 3. The yield and quality performance of flue-cured tobacco under each treatment were measured and analyzed. The results showed that the single-leaf weight of Formula 1，Formula 2，and Formula 3 was significantly （p<0.05） higher than that of the control，with increases of 22.32%，24.71%，and 16.83%，respectively. The oil content scores of appearance quality were improved compared with the control，and the yields of petroleum ether extract were significantly increased in all three formula treatments. The output values of Formula 1 and Formula 2 were significantly increased by 12.89% and 10.86%，respectively，compared with the control. Sensory quality analysis of flue-cured tobacco leaves indicated that Formula 1 performed the best among all treatments. In conclusion，Formula 1 significantly enhanced the oil content of flue-cured tobacco leaves and showed the best overall improvement in yield and quality，making it a promising formulation for enhancing oil content，chemical quality，and yield of flue-cured tobacco in the Xuchang tobacco-growing area in central Henan. Figures and Tables | References | Related Articles | Metrics

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Distribution characteristics of chemical components and quality traits in different leaf zones of tobacco based on fractal theory LI Peiyan, WEI Yuewei, XU Shizhen, LI Yanzhou, YIN Quanyu, REN Tianbao, GENG Zongze, LIU Guoshun 2026, 67 (9):  2149-2156.  DOI: 10.16178/j.issn.0528-9017.20250492 Abstract ( 39 )   HTML ( 0 )   PDF (1564KB) ( 139 )   In order to investigate the differences and distribution patterns of chemical components and quality characteristics in different leaf positions of flue-cured tobacco leaves，this study took tobacco leaves as the test material， cut the leaves into 14 different zones from the tip to the base of the leaf， and tobacco strips were classified into five categories：large strip， medium strip， small strip， broken strip and fine dust，according to the size grade. Precise chemical composition analysis，cluster analysis，sensory quality evaluation were conducted on these samples. The results showed that the chemical component contents of flue-cured tobacco leaves varied greatly among different leaf positions and the size grade of tobacco strips，with obvious variation patterns from leaf tip to leaf base. The contents of total sugar and reducing sugar gradually decreased from the leaf margin to the main vein，while the contents of nicotine and total nitrogen were higher in the leaf tip and leaf margin，and potassium，chlorine and pigment contents were more enriched in the leaf base. Based on the overall chemical components，the leaves can be clustered into two major categories：zones 1-7，9，10，and large strip，medium strip，broken strip，fine dust，and small strip，as well as zones 8 and 11-14. In practical production，tobacco leaves with similar clustering results can be substituted for one another，thereby alleviating the shortage of certain tobacco raw materials. The results of the study clarified the distribution patterns of chemical components in different positions of flue-cured tobacco leaves，and provided a theoretical basis for improving the refined processing of flue-cured tobacco and enhancing the utilization value of tobacco raw materials. Figures and Tables | References | Related Articles | Metrics

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Experimental study on the rapeseed-ratoon rice cropping system and lightweight simplified cultivation techniques LIU Weiwei, LIN Guanghao, WANG Yumeng 2026, 67 (9):  2157-2162.  DOI: 10.16178/j.issn.0528-9017.20250734 Abstract ( 51 )   HTML ( 0 )   PDF (442KB) ( 175 )   To screen for superior varieties suitable for the “rapeseed-ratoon rice” cropping system and to explore the associated simplified cultivation techniques，this study conducted variety comparison trials and demonstration validation involving 8 rapeseed varieties and 5 ratoon rice varieties in Changshan County，Zhejiang Province. The results showed that Fengyou 737（yield 1 533.0 kg·hm-2，growth duration 180 d），Fengyou 112（1 530.0 kg·hm-2，178 d），and Yangguang 131（1 416.0 kg·hm-2，177 d）were early-maturing and high-yielding rapeseed varieties. Yongyou 4901 had a total growth duration of 210-215 d across the main and ratoon seasons with a combined yield of 14 008.5 kg·hm-2，while Yongyou 123 had a total growth duration of 212-217 d with a combined yield of 13 919.3 kg·hm-2，both demonstrating outstanding yield potential. Due to its shorter growth duration，Yongyou 4901 is more suitable for annual cultivation in combination with rapeseed. The demonstration trials further validated the high yield potential and adaptability of the selected variety combinations，and the corresponding simplified cultivation techniques were summarized. Figures and Tables | References | Related Articles | Metrics

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Effects of calcium and boron on fruit quality and storage tolerance of Chinese bayberry SUN Li, WANG Rong, ZHANG Shuwen, YU Zheping, ZHANG Yang, QU Jing, ZHANG Qi 2026, 67 (9):  2163-2167.  DOI: 10.16178/j.issn.0528-9017.20260473 Abstract ( 42 )   HTML ( 1 )   PDF (412KB) ( 152 )   To investigate the effects of calcium and boron on fruit quality and storage tolerance of Chinese bayberry，the cultivars Biqizhong and Dongkui were used as test materials，and foliar sprays of boron and calcium were applied separately during fruit development，with water spray as the control. The external and internal quality indicators of fruits at the color-turning stage and the mature stage were measured. The results showed that both exogenous boron and calcium treatments improved fruit quality. Boron treatment increased soluble sugar content at the color-turning stage，reduced titratable acidity，and significantly （p<0.05）increased amino acid content at the mature stage. Calcium treatment significantly increased fruit firmness，total soluble solids content，and anthocyanin content，with particularly prominent effects on improving fruit color and firmness. Overall，both calcium and boron improved the internal quality of Chinese bayberry fruits，but calcium showed better effects on improving storage tolerance. In conclusion，application of calcium and boron during the fruit stage can effectively improve Chinese bayberry fruit quality and has practical value for production. This study provides a theoretical basis for quality regulation and storage tolerance improvement in Chinese bayberry cultivation management. Figures and Tables | References | Related Articles | Metrics

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Comparison and screening of agronomic traits of 13 medium-sized fruit tomato varieties in Cixi region YAO Zhuping, LIU Chenxu, CHENG Yuan, WAN Hongjian, WANG Rongqing, RUAN Meiying, ZHOU Guozhi, LI Zhimiao, YE Qingjing 2026, 67 (9):  2168-2171.  DOI: 10.16178/j.issn.0528-9017.20260411 Abstract ( 49 )   HTML ( 1 )   PDF (348KB) ( 162 )   To screen high-quality medium-sized fruit tomato varieties suitable for cultivation in the Cixi region，a systematic comparison of the main agronomic traits of 13 varieties was conducted. A randomized block experiment was carried out in a protected greenhouse in Kandun Sub-District，Cixi City，from 2025 to 2026. The botanical traits，fruit traits，and yield components were measured for each variety. The results showed that all 13 varieties exhibited pink fruit color. Zhewei 4 had the highest number of flowers per ear（9.2 flowers）. S25001 displayed the largest fruit transverse diameter（8.1 cm）and the greatest single fruit weight（194.0 g）. Yuyi Suantianguo had the highest soluble solids content（8.8%），while Zhewei 4 showed the lowest malformed fruit rate（10.22%）. S25001 achieved the highest 667 m2 yield （6 914.2 kg），and S24010 produced the lowest 667 m2 yield（1 837.4 kg）. Considering the overall performance of all traits，Zhewei 4 had a high number of fruit setting and a low malformed fruit rate，and Zhewei 2 showed well-balanced comprehensive traits. Therefore，both varieties can be recommended as main-promoting fruit tomato varieties for further demonstration and extension in the Cixi region. Figures and Tables | References | Related Articles | Metrics

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Comparative study of six taste-oriented tomato varieties under greenhouse substrate cultivation YE Xiongfang, CHEN Ke, HE Dongping, RONG Song, XIANG Tao, GU Likai, YU Yongqi, FAN Anli, YANG Zhe, ZHANG Yulong, WANG Huaying 2026, 67 (9):  2172-2176.  DOI: 10.16178/j.issn.0528-9017.20260059 Abstract ( 41 )   HTML ( 0 )   PDF (429KB) ( 153 )   To identify high-quality taste-oriented tomato varieties suitable for substrate cultivation in greenhouses in Xiaoshan District，this study introduced six mainstream market varieties. Through systematic measurement and comprehensive evaluation of key indicators，including phenophase，disease resistance，fruit traits，yield，and commercial value， the adaptability and production potential of each variety under local greenhouse conditions were assessed. Results demonstrated that Jiahong 100 exhibited the most outstanding performance，combining high yield（4 228.95 kg per 667 m2），strong disease resistance（pathogen indices for viral disease and stem rot both at 0.2），and excellent commercial quality（visual score of 0.8），making it highly adaptable. Guanghui 101 stood out with its high sugar content（10.78 °Brix）and superior flavor profile（sensory score of 8.9），making it ideal for premium markets. Based on these findings，Jiahong 100 was recommended as the primary tomato variety for substrate cultivation in Xiaoshan's greenhouses，while Guanghui 101 served as a complementary variety. Figures and Tables | References | Related Articles | Metrics

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Effects of spraying plant growth regulator and foliar fertilizer on the fruit cracking and quality of hybrid citrus Yangguang 1 MA Chuangju, WANG Dengliang, SUN Jiancheng, CHEN Jun, XU Yanhui, TONG Lu, LIU Lili 2026, 67 (9):  2177-2182.  DOI: 10.16178/j.issn.0528-9017.20250414 Abstract ( 46 )   HTML ( 0 )   PDF (456KB) ( 156 )   Using hybrid citrus Yangguang 1 as the experimental material，plant growth regulators and foliar fertilizers were sprayed in the early flowering period（beginning of April），full flowering period（mid-April）and fruit enlargement period（mid-July）. The effects of spraying plant growth regulators and foliar fertilizers at different growth periods on fruit cracking and quality were investigated. The results showed that compared with the control，each treatment could significantly （p<0.05）reduce the fruit cracking rate of Yangguang 1 and significantly increase the single fruit weight. The peel thickness of T4 treatment ［spraying 30 μg·L-1 6-benzylaminopurine （6-BA）+50 μg·L-1 gibberellic acid （GA?） at the early flowering period， spraying 100 μg·L-1 GA3 at the full flowering period，and spraying 800 times Ca-Mg-B-Zn-Fe fertilizer at the fruit enlargement period］ was significantly reduced. T3 （spraying 30 μg·L-1 6-BA+50 μg·L-1 GA? at the early flowering period， spraying 100 μg·L-1 GA3 at the full flowering period，and spraying 500 times potassium dihydrogen phosphate at the fruit enlargement period）and T4 treatments improved the color contribution index of fruit. Except for the decrease of solid-acid ratio of T7 treatment （spraying 200 μg·L-1 GA?+500 times potassium dihydrogen phosphate at the fruit enlargement period），the solid-acid ratio and vitamin C content of other treatments had no significant difference compared with the control. Principal component analysis showed that T3 treatment had the highest comprehensive score and overall effectiveness，which can be referenced for practical production. Figures and Tables | References | Related Articles | Metrics

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Effects of chemical fertilizer replacement by bio-nano-selenium fertilizer on fruit quality of Hongmeiren citrus and soil nutrients FAN Mingliang, FENG Kongjun, ZHOU Kaile, YANG Shuai, HE Bing, ZHOU Yong, PAN Feiyun, ZHENG Lin, LAN Liyan, FU Ruijie, DAI Fen 2026, 67 (9):  2183-2186.  DOI: 10.16178/j.issn.0528-9017.20250377 Abstract ( 53 )   HTML ( 1 )   PDF (408KB) ( 144 )   To explore the potential of bio-nano-selenium fertilizer as a replacement for chemical fertilizer in improving fruit quality of Hongmeiren citrus and enhancing soil nutrients，this study compared and analyzed the effects of different replacement ratios of bio-nano-selenium fertilizer. The results showed that the application of bio-nano-selenium fertilizer increased the selenium content，single fruit weight，soluble solid content，and sugar-acid ratio in the fruit. Considering all indicators，treatment T5（60% replacement of chemical fertilizer by bio-nano-selenium fertilizer）ranked first overall，with its yield increasing by 45.70% compared with CK. Under this treatment，soil nutrient contents were more appropriate，maintaining soil nitrogen content，alleviating phosphorus loss，and keeping a relatively high potassium content. This study can provide a reference for reducing chemical fertilizer application，improving quality and efficiency in Hongmeiren citrus production. Figures and Tables | References | Related Articles | Metrics

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Comprehensive evaluation of salt tolerance of local wild grape resources from Fu’an City LIN Xu, LIN Xiaohong, DONG Yu, WANG Xianyin, FAN Xiaohui 2026, 67 (9):  2187-2192.  DOI: 10.16178/j.issn.0528-9017.20250565 Abstract ( 36 )   HTML ( 1 )   PDF (481KB) ( 141 )   To explore the salt tolerance of local wild grape resources and screen suitable salt tolerant rootstock varieties for grape production areas along the southeast coast，10 local wild and progeny grape germplasm resources from Fu’an City were selected for salt tolerance experiments，with summer black grape as the control. Irrigation with 0.1 mol·L-1 NaCl solution was used to measure various indicators of salt damage index，new shoot growth，antioxidant enzyme activity，osmoregulatory substances，malondialdehyde content of seedlings under salt（NaCl）stress. Salt tolerance of wild grape resources was comprehensively analyzed by principal component analysis，membership function analysis. The results showed that the activities of catalase （CAT），peroxidase （POD），and superoxide dismutase （SOD） of S27 and S08 were relatively high in the control group and under NaCl stress. S27，S08， and S05 occupied the top three positions in the comprehensive ranking，indicating strong salt tolerance and suitability as salt tolerant grape rootstock resources in southeastern coastal areas such as Fu’an. Figures and Tables | References | Related Articles | Metrics

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Effects of Trichoderma microbial agent on grape quality，yield and soil microorganisms WANG Lili, ZHU Shijun 2026, 67 (9):  2193-2195.  DOI: 10.16178/j.issn.0528-9017.20260081 Abstract ( 57 )   HTML ( 1 )   PDF (369KB) ( 148 )   Yongyou grapes were used as the experimental materials to explore the effect of self-made Trichoderma microbial agent on grape quality，yield，and rhizosphere soil microbial community. The results showed that the application of self-made non-inactivated Trichoderma microbial agents significantly（p<0.05）improved grape fruit quality，with a significant increase of 28.93% in cluster weight and 13.00% in vitamin C content compared with CK （pure water）. The yield of non-inactivated Trichoderma microbial agent treatment was the highest，significantly increasing by 20.68% compared with CK. In terms of microbial community，the number of soil bacteria significantly increased by 17.13% and the number of fungi significantly decreased by 16.84% treated with non-inactivated Trichoderma microbial agents compared with CK. The results of this study can provide a reference for the promotion of microbial agent. Figures and Tables | References | Related Articles | Metrics

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A method for inducing haploid plants from spiral pepper XU Zheming, WU Shuinyu 2026, 67 (9):  2196-2199.  DOI: 10.16178/j.issn.0528-9017.20250599 Abstract ( 40 )   HTML ( 1 )   PDF (380KB) ( 150 )   Anther culture is the primary method for obtaining haploid plants in pepper（Capsicum annuum L.），but it is highly genotype-dependent，and there is currently no established anther culture protocol for spiral pepper. In this study，the spiral pepper variety Bolong 37-94（Rijk Zwaan）was used as experimental material. Through screening of hormone types，optimization of hormone combinations，and pre-culture treatments，a suitable anther culture system for spiral pepper was established. Anthers of spiral pepper were collected，surface-sterilized，and then transferred to D6 medium（MS+0.5 mg·L-1 IAA+1.0 mg·L-1 NAA+2.0 mg·L-1 2，4-D+1.0 mg·L-1 KT30），followed by incubation at 35 ℃ for one day. Subsequently，the anthers were cultured under a photoperiod of 16 hours，at 24 ℃，with a light intensity of 2 000 lx until embryoids were formed. The embryoids were then transferred to MS medium and cultured until regenerated plantlets were obtained. The anther culture protocol established in this study achieved an embryoid induction rate of 5.2%. Ploidy analysis by flow cytometry confirmed that haploid plants were successfully obtained among the regenerated plantlets. This protocol provides an effective approach for accelerating pure line selection and genetic population construction in pepper. Figures and Tables | References | Related Articles | Metrics

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Effects of 10 kinds of microbial inoculants on the growth，development and yield of garlic GAO Yuanyuan, TAN He, LI Siqi, ZHU Chunyu, HE Junya, LIU Guowei, REN Yanyun 2026, 67 (9):  2200-2204.  DOI: 10.16178/j.issn.0528-9017.20250298 Abstract ( 54 )   HTML ( 0 )   PDF (415KB) ( 155 )   The effects of 10 kinds of microbial inoculants on the growth and yield of garlic were analyzed with water as the control. The results showed that after the application of microbial inoculants，garlic plants had strong growth potential，robust plants，and high yield of garlic shoots and bulbs，among which jinggangmycin and zhongshengmycin had the most significant yield increase，reaching more than 1 500 kg per 667 m2. The yield increases of 50% phenazine-1-carboxylic acid，Bacillus subtilis，Trichoderma harzianum，Bacillusamyloliquefaciens and polyoxin ranged from 3.47% to 7.25%. The results of this study provided a reference for the scientific application of microbial inoculants on garlic. Figures and Tables | References | Related Articles | Metrics

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Study on entity recognition and knowledge graph application for beet breeding in low-resource scenarios ZHANG Li, CHEN Junjie, WANG Xiaoyu 2026, 67 (9):  2205-2210.  DOI: 10.16178/j.issn.0528-9017.20260443 Abstract ( 35 )   HTML ( 0 )   PDF (590KB) ( 141 )   Beet is an important sugar crop in China，and its breeding literatures contain rich structured knowledge such as varieties，traits，and genes. However，the scarcity of annotated data and the complexity of terminology limit the performance of existing entity recognition methods. To address this problem，we constructed the first dedicated dataset for named entity recognition（NER）in beet breeding in China，defining eight core entity types. Through a strategy combining small-scale manual annotation with rule-based template augmentation，the strategy effectively expanded the training samples. Using this dataset，the BERT-BiLSTM-CRF model achieved an F1 value of 90.19%，outperforming baseline model. To validate the practicality of the proposed method，we applied the model to recognize entities in 502 literatures and built a knowledge graph that centered on the literature and supports traceability. The graph's coverage and retrieval accuracy were evaluated through structured-retrieval tasks. Experimental results showed that the normalized retrieval accuracy based on the graph reached 90.8%，confirming the usability of the knowledge graph in the domain of beet breeding. This study provides an effective methodological reference for entity recognition and knowledge organization in low-resource scenarios. Figures and Tables | References | Related Articles | Metrics

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Physiological and biochemical responses of Clematis Midori under waterlogging stress ZHANG Weiguo, ZHANG Haiying, HUANG Aiyun, LIN Lin, HU Yaping, HU Qingdi, ZHANG Xule, CHEN Yizeng 2026, 67 (9):  2211-2217.  DOI: 10.16178/j.issn.0528-9017.20260260 Abstract ( 41 )   HTML ( 0 )   PDF (1185KB) ( 145 )   To analyze the tolerance characteristics of Clematis to waterlogging stress，this study used Clematis Midori as the experimental material and set up three treatments：control，half-waterlogging，and full-waterlogging. The growth phenotype，photosynthetic characteristics，chlorophyll content，malondialdehyde（MDA）content，antioxidant enzyme activity，and 1-aminocyclopropane-1-carboxylic acid（ACC）and ATP contents of Clematis under waterlogging stress were systematically analyzed. The results showed that full-waterlogging stress inhibited the growth of Clematis Midori，with obvious wilting and leaf abscission，compared with the control，the net photosynthetic rate significantly（p<0.05）decreased by 84.55%，total chlorophyll content significantly decreased by 28.15%，MDA content significantly increased by 53.22%，ACC content significantly decreased by 69.92%，and ATP content significantly decreased by 84.65%. Meanwhile，the activities of antioxidant enzymes such as peroxidase and catalase were significantly increased. ACC content reached the highest value under half-waterlogging stress. These results indicate that Clematis can initiate certain adaptive responses through ACC accumulation and the differential regulation of the antioxidant enzyme system under waterlogging stress. However，continuous severe waterlogging leads to a sharp decline in energy metabolism，aggravated membrane lipid peroxidation，and severe damage to the photosynthetic system，exceeding the physiological tolerance threshold of the plant and significantly inhibiting growth. These findings provide a theoretical basis for the breeding of stress-resistant varieties and cultivation management of Clematis. Figures and Tables | References | Related Articles | Metrics

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Evaluation of agronomic traits among 28 Pelargonium hortorum germplasm resources ZHANG Fujian, SANG Liangliang, ZHANG Ping 2026, 67 (9):  2218-2223.  DOI: 10.16178/j.issn.0528-9017.20250563 Abstract ( 58 )   HTML ( 1 )   PDF (443KB) ( 181 )   To investigate the performance and variability in growth habits and agronomic traits among different Pelargonium hortorum germplasms，28 Pelargonium hortorum germplasm resources were selected as experimental materials. Agronomic traits were observed and measured，followed by comprehensive analysis using one-way ANOVA analysis，correlation analysis，variability analysis，and clustering analysis.The results showed that A06 had the highest plant height，reaching 13.87 cm，while the maximum stem thickness was 12.86 mm（C03）and the minimum was 8.06 mm（A01）. A07 demonstrated the highest branch number（4.00 branches），A06 showed the greatest leaf width（12.80 cm），and A02 recorded the highest relative chlorophyll content（SPAD value），reaching 48.43. Flower number in 28 Pelargonium hortorum germplasm resources ranged from 4.33 to 13.33，with B series varieties displaying higher flower numbers than other series. A06 had the longest pedicel（20.00 cm），while the shortest was B01（9.53 cm）. The average coefficient of variation（CV）for the nine agronomic traits was 15.94%，with the flower number exhibiting the highest coefficient of variation at 33.98%. Further analysis revealed that plant height showed a highly significant（p<0.01）positive correlation with crown width and pedicel length，and stem thickness exhibited a highly significant positive correlation with crown width and leaf width. Crown width demonstrated a highly significant positive correlation with branch number，leaf width，and pedicel length. Plant height showed significant（p<0.05）positive correlation with stem thickness，pedicel length showed significant positive correlation with stem thickness and leaf width. Notably，SPAD value displayed a highly significant negative correlation with crown width and leaf width. Furthermore，hierarchical cluster analysis based on nine agronomic traits categorized 28 Pelargonium hortorum accessions into six distinct groups at a distance coefficient of 15. Group Ⅱ exhibited the largest stem thickness，crown width，leaf width，and pedicel length，while Group Ⅲ performed best in plant height and branch number. Group Ⅴ had the highest leaf number and superior leaf utility，whereas Group Ⅵ demonstrated the highest SPAD value and flower number，indicating vigorous reproductive growth and higher photosynthetic efficiency. This study provides theoretical reference for the breeding and cultivation management of Pelargonium hortorum. Figures and Tables | References | Related Articles | Metrics

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The relationship between the flowering period of Paeonia suffruticosa Andr and its phenotype as well as environmental factors WANG Ruohan, JI Hanle, YAO Junqiao, DING Jianlan, MA Huiping, WANG Erqiang 2026, 67 (9):  2224-2229.  DOI: 10.16178/j.issn.0528-9017.20250258 Abstract ( 42 )   HTML ( 2 )   PDF (452KB) ( 150 )   Flowering phenology is a critical characteristic of ornamental peonies. Investigating the growth patterns of flowering characteristics across different peony varieties and their relationships with environmental factors can provide valuable insights for precise flowering period regulation，identification of unique germplasm resources，and targeted breeding programs.This study employed 29 ornamental peony varieties from the Luoyang National Peony Germplasm Resource Garden as experimental materials，investigating their flowering characteristics and measuring data on petal traits and meteorological factors during flowering periods. The results showed that the flowering period of the tested peony varieties ranged from 7 to 13 days，with a coefficient of variation of 17.43%. Correlation analysis indicated that flowering duration exhibited a highly significant （p<0.01） positive correlationwith single-branch flower formation rate and petal thickness；a highly significant negative correlation with light intensity and daily maximum temperature；a significant （p<0.05） negative correlation with daily average temperature；while no significant correlations were observed with indicators such as flower color，flower shape，petal number，or flower diameter. The results of this study provided valuable references for resource evaluation of ornamental peony varieties，precise regulation of flowering periods，identification of unique germplasm resources，and targeted breeding. Figures and Tables | References | Related Articles | Metrics

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Population dynamics of fly insects in citrus orchards in Lishui HU Manfei, YAN Fuhua, LI Yang, YANG Xianyu, WU Lianhai 2026, 67 (9):  2230-2234.  DOI: 10.16178/j.issn.0528-9017.20250568 Abstract ( 39 )   HTML ( 1 )   PDF (442KB) ( 145 )   Fly insects are important pests in the citrus industry. In order to understand the occurrence pattern of fly insects in Lishui area，three representative citrus orchards were selected to use pheromone-baited traps and fly adhesive boards for trapping pests. The results showed that three species of fly insects in Lishui orchards：Bactrocera dorsalis，Zeugodacus tau，and Z. scutellatus，were trapped，with the first two being the dominant species. B. dorsalis peaked synchronously with citrus maturity，identifying it as the primary yield-reducing pest. Z. tau persisted year-round but developed a single distinct peak from late November to early December due to climatic constraints. This peak occurred later than the maturity period of citrus and did not overlap with B. dorsalis，causing no economic damage. Z. scutellatus accounted less than 3.0% of the total trapped population，warranting no specific control measures. Population dynamics were consistent across all monitoring sites. This study systematically elucidates the occurrence pattern of fly insects for the first time in citrus orchards in Lishui，providing reference for the prevention and control of fly insects in the local area. Figures and Tables | References | Related Articles | Metrics

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Identification and pathogenicity of pathogens of garlic（Allium sativum L.） root rot GUO Lianjun, WANG Yajing, LI Teng, CUI Kai, DONG Zhan 2026, 67 (9):  2235-2239.  DOI: 10.16178/j.issn.0528-9017.20260357 Abstract ( 42 )   HTML ( 0 )   PDF (1165KB) ( 139 )   From garlic root rot samples collected in Shanghe County，Shandong Province，a total of 20 isolates were obtained. These isolates were identified using a combination of morphological and molecular approaches，and their pathogenicity to garlic seedlings was assessed through soil inoculation. The results showed that 17 isolates were identified as Fusarium oxysporum，and three isolates as F. ipomoeae. Both Fusarium species exhibited pathogenicity to garlic seedlings，confirming them as causal agents of garlic root rot. Among them，F. oxysporum displayed stronger pathogenicity，with average diseased plant rate and disease index reaching 95.24% and 59.59，respectively；whereas F. ipomoeae showed weaker pathogenicity，with average diseased plant rate and disease index of 66.67% and 20.32，respectively. Notably，the identification of F. ipomoeae as a pathogen of garlic root rot represented the first report of its kind，providing a theoretical basis for disease-resistant breeding and field control of garlic root rot. Figures and Tables | References | Related Articles | Metrics

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Survey of weed communities in wheat fields in northern Zhejiang and analysis of their changing causes XIE Jing, TIAN Ye, YAO Xiaoming, SHEN Ying, WANG Siming, LI Bin, NING Guoyun 2026, 67 (9):  2240-2244.  DOI: 10.16178/j.issn.0528-9017.20250420 Abstract ( 48 )   HTML ( 0 )   PDF (398KB) ( 141 )   In recent years，the promotion of large-scale wheat production and no-till techniques have led to less intensive field management，exacerbating weed infestations and significantly increasing control challenges. To investigate the characteristics and evolutionary mechanisms of weed community changes in wheat fields and to provide a basis for scientific control strategies，a survey was conducted in northern Zhejiang in 2024.Results showed that Alopecurus aequalis，Beckmannia syzigachne，Persicaria orientalis，and Myosoton aquaticum were the dominant weed species. Analysis revealed that shifts in planting methods，the spread of malignant weeds，changes in weed control techniques，and herbicide resistance due to prolonged use of single herbicides were key drivers of weed community succession. Therefore，it was recommended to reduce the weed seed bank in shallow soil through deep plowing，prioritize post-sowing soil sealing techniques，and establish an integrated control strategy combining agricultural measures and chemical control to enhance weed management effectiveness. Figures and Tables | References | Related Articles | Metrics

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Effects of different operational parameters of unmanned aerial vehicle on control efficacy against rice planthoppers LI Kuan, XU Xiaofang, WANG Chunyan, ZHANG Chao, JIANG Minhua, WANG Xing, DING Yi 2026, 67 (9):  2245-2249.  DOI: 10.16178/j.issn.0528-9017.20250522 Abstract ( 45 )   HTML ( 2 )   PDF (389KB) ( 148 )   To investigate the effects of different operational parameters of plant protection unmanned aerial vehicle（UAV）on the control efficacy against rice planthoppers，field trials were conducted during the booting stage of early-season rice and the tillering stage of late-season rice in Jinhua City，Zhejiang Province，China. The effects of spray volume，operating height，and flight speed on control efficacy were analyzed. The results showed that spray volume was the primary factor affecting the control efficacy of UAV. At the booting stage，increasing spray volume from 2 L to 4 L per 667 m2 improved the control efficacy by 19.95-25.59 percentage points；at the tillering stage，a spray volume of 3 L per 667 m2 was sufficient to achieve the optimal control efficacy. The effect of operating height on control efficacy varied with growth stage：at the booting stage，operating heights of 2-3 m provided relatively stable control efficacy；at the tillering stage，increasing operating height from 2 m to 4 m resulted in considerable differences in control efficacy. Flight speeds of 4 m·s-1 and 5 m·s-1 showed no significant difference in control efficacy. Under appropriate operational parameters，UAV application achieved control efficacy comparable to that of knapsack electric sprayer，with no adverse effects on rice growth. In conclusion，the recommended operational parameters for UAV application at the tillering stage of rice are operating height of 2 m，spray volume of 3 L per 667 m2，and flight speed of 4 m·s-1；at the booting stage，the recommended parameters are operating height of 2-3 m，spray volume of 4 L per 667 m2，and flight speed of 4-5 m·s-1. Figures and Tables | References | Related Articles | Metrics

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Effects of different fertilizer treatments on Pomacea canaliculata and Oryza sativa HE Jie, ZHANG Juefeng, LI Fang, YU Kaili, LI Linhan, WANG Jia, ZHONG Haiying 2026, 67 (9):  2250-2254.  DOI: 10.16178/j.issn.0528-9017.20250607 Abstract ( 48 )   HTML ( 2 )   PDF (438KB) ( 151 )   Pomacea canaliculata（golden apple snail）is a major pest of aquatic crops. It has high fecundity and disperses rapidly with water flow. Current control measures rely primarily on chemical molluscicides，which to some extent affect water quality in paddy fields and the safety of other aquatic organisms. Therefore，there is an urgent need to develop efficient and environmentally friendly “green” control strategies against this pest. In this study，we compared the effects of different fertilizer treatments， namely，ammonium bicarbonate applied as base fertilizer，ammonium bicarbonate applied as base fertilizer plus one topdressing， tea seed cake，and tobacco stem organic fertilizer， on snail mortality as well as on rice yield and agronomic traits in paddy fields in Huangyan District，Taizhou City. The results showed that all the tested fertilizer treatments exhibited certain molluscicidal effects. Among them，tea seed cake applied at 300 kg·hm-2 achieved the highest snail mortality rate of 43.38%. In terms of rice yield，all the tested treatments resulted in higher grain yields compared with the conventional fertilizer control. The highest yield was observed under the tea seed cake treatment at 375 kg·hm-2，with an increase of 1 521.20 kg·hm-2（20.37%）over the control，followed by tobacco stem organic fertilizer at 300 kg·hm-2，which gave a yield increase of 19.83%. Under different application rates of tea seed cake，both the effective panicle number and 1 000-grain weight remained at relatively high levels. The treatment of ammonium bicarbonate as basal fertilizer at 375 kg·hm-2 showed the highest net benefit increase of 3 345.29 yuan·hm-2，whereas the three application amounts of ammonium bicarbonate as one basal plus one topdressing all exhibited lower benefit increases than the other fertilizer treatments. Collectively，these findings indicate that the tested fertilizer treatments have promising potential in controlling P. canaliculata while simultaneously improving rice yield and economic returns，thus providing a valuable reference for the green management of this snail in aquatic crop fields. Figures and Tables | References | Related Articles | Metrics

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Effects of different disinfection methods on soil microbial flora of continuous cropping strawberry CAO Yiwen, SUN Ping, WU Jiaqi, CHEN Chenfei, LIN Xianrui, SHEN Jiansheng 2026, 67 (9):  2255-2264.  DOI: 10.16178/j.issn.0528-9017.20250376 Abstract ( 43 )   HTML ( 0 )   PDF (2394KB) ( 166 )   This study investigated the effects of three soil disinfection methods， irrigation with 10 mg·L-1 ozone water（R1），irrigation with 150 mg·L-1 hypochlorous acid solution（R2），and high-temperature sterilization at 121 °C for 20 min（R3）， on soil microbial communities in strawberry soil with 15 years of continuous cropping. The results showed that，compared with the water control（R4），R3 caused the bacterial Chao1 index，observed OTUs，and Shannon index to first decrease and then increase，while it reduced the fungal Chao1 index，observed OTUs，and Shannon index. In contrast，R1 and R2 increased the bacterial Chao1 index，observed OTUs，and Shannon index initially，followed by a decrease，and increased the fungal Chao1 index，observed OTUs，and Shannon index. Disinfection with ozone water inhibited the growth of harmful bacteria and increased the abundance of beneficial microorganisms with soil remediation capabilities. Although R2 increased the relative abundance of Chitinophagaceae，which promotes plant growth and development，it also led to higher abundances of pathogenic genera such as Fusarium and Nectriaceae. High-temperature sterilization imposed severe stress on all soil microorganisms and affected both bacterial and fungal community structures. Figures and Tables | References | Related Articles | Metrics

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Identification of Watermelon iflavirus 1（WIV1） WANG Haoran, WANG Yucong, XIAO Ming, MING Ke, WU Huijie, FENG Guozhong 2026, 67 (9):  2265-2269.  DOI: 10.16178/j.issn.0528-9017.20250824 Abstract ( 40 )   HTML ( 0 )   PDF (723KB) ( 150 )   This study aimed to identify the pathogen responsible for typical viral disease symptoms observed in watermelon plants in Zhejiang Province，including vein distortion，leaf crumpling，and downward curling. Total RNA extracted from symptomatic leaves was subjected to meta-transcriptomic sequencing. Through sequence assembly and alignment，a novel virus species belonging to the family Iflaviridae was identified and named Watermelon iflavirus 1（WIV1）. Subsequent RT-PCR and DNA sequencing validation detected specific sequences of this virus in 7 out of 10 diseased leaf samples，yielding a positive detection rate of 70%. Further genomic annotation revealed that WIV1 encoded a single open reading frame，and the putative polyprotein shared high amino acid sequence homology with polyproteins of known members in the family Iflaviridae，consistent with the characteristics of typical Iflavirus. For the first time，this study reports the infection of watermelon plants in China by WIV1，providing a theoretical basis for pathogen identification，pathogenesis research，and the development of integrated control strategies for this viral disease. Figures and Tables | References | Related Articles | Metrics

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Improvement and validation of an HPLC method for the determination of vitamin C in fruits and vegetables CHEN Guo 2026, 67 (9):  2270-2274.  DOI: 10.16178/j.issn.0528-9017.20260181 Abstract ( 38 )   HTML ( 0 )   PDF (491KB) ( 142 )   To address the issues of oxidative degradation of vitamin C and severe chromatographic interference in fruit and vegetable matrices，this study optimized the reduction procedure and chromatographic conditions of the existing high-performance liquid chromatography（HPLC）method. The results showed that limits of detection for L-ascorbic acid and D-isoascorbic acid before reduction，and for L-ascorbic acid and D-isoascorbic acid after reduction，were as low as 0.14，0.13，0.35，and 0.32 mg·kg-1，respectively. Both isomers exhibited good linearity within the respective concentration ranges，with coefficient of determination（R2）of no less than 0.999 9. In the three-level（low，medium，and high）spiked recovery experiments（n=7），the recovery rates ranged from 90.0% to 106.0%，with relative standard deviations of no more than 2.5%. The improved method is simple in operation with less matrix interference，and can simultaneously determine the content of vitamin C before and after oxidation，making it suitable for batch detection and routine application for fruit and vegetable samples. Figures and Tables | References | Related Articles | Metrics

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Application of graphite furnace atomic absorption spectrometry for determining lead content in high-salt foods XIANG Qianqian, ZHANG Xiaotian, WU Mengxi, ZHANG Ying, DENG Ying, LIU Chen, HUANG Wenyao 2026, 67 (9):  2275-2278.  DOI: 10.16178/j.issn.0528-9017.20250479 Abstract ( 36 )   HTML ( 1 )   PDF (367KB) ( 136 )   Addressing the cumbersome and technically challenging pre-treatment steps involved in the graphite furnace atomic absorption spectrometry method specified in the National Food Safety Standard for Determination of Lead in Foods（2023 edition），this study proposed a simplified and improved protocol. The standard-recommended palladium nitrate-ammonium dihydrogen phosphate ［Pd（NO?）?］ matrix modifier was replaced with ammonium dihydrogen phosphate （NH?H?PO?） or ammonium nitrate （NH?NO?），with ashing temperatures set at 600 and 500 ℃， respectively，eliminating the need for additional solid-phase extraction column purification. Method accuracy was validated through quality control samples and addition recovery tests. The results demonstrated that both matrix modifiers maintained a good linear relationship between lead concentration（0-25.0 μg·L-1）and absorbance. When NH4H2PO4 was used as the modifier，the addition recovery rates for salt and hot pot base were 99.1% and 97.6%，respectively；whereas with NH4NO3，the recovery rate for salt reached 99.2%，but was lower at only 84.3% for hot pot base. Furthermore，when NH4H2PO4 was employed as the modifier at a calcination temperature of 600 ℃，the simulated high-salt samples exhibited lead concentrations of 0.126 and 1.083 mg·kg-1，both within the specified limits of the control samples. These findings confirm that the modification method was simple to implement，accurate，and reliable，effectively reduces pre-treatment complexity，is suitable for rapid lead determination in high-salt foods，and is well-suited for widespread application. Figures and Tables | References | Related Articles | Metrics

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Effects of selenomethionine on the growth performance，immune function and serum biochemical indicators of yellow-feathered broilers induced by high fluoride exposure ZHANG Kun, ZHONG Qunbin, WU Hao, LI Jun, SHEN Yutian, WANG Yongxia 2026, 67 (9):  2279-2284.  DOI: 10.16178/j.issn.0528-9017.20260291 Abstract ( 39 )   HTML ( 0 )   PDF (431KB) ( 149 )   To investigate the regulatory effect of selenomethionine（SM）on the physiological status of broilers under high fluoride-induced oxidative stress conditions，this experiment selected yellow-feathered broilers with oxidative stress as the research subjects，and evaluated the effects of adding SM to the diet on growth performance，immune indicators，and serum biochemical indicators. 720 one-day-old Lingnan yellow-feathered broilers were randomly divided into 4 groups：control group（CON），sodium fluoride （HF） group，sodium selenite（SS） group，and SM group. The HF group added 800 mg·kg-1 sodium fluoride to the basal diet，while the SS group and SM group supplemented 0.15 mg·kg-1 SS and SM on the basis of the HF diet. The experimental period was 56 days. The results showed that compared with the CON group，the average daily weight of the HF group was significantly（p<0.05）reduced，the feed-to-weight ratio was significantly increased，the serum immunoglobulin levels and immune organ indices were significantly decreased，and the serum alanine aminotransferase activity，aspartate aminotransferase activity，lactate dehydrogenase activity，glucose content，and uric acid content were significantly increased. Compared with the HF group，selenium supplementation could improve the above changes to varying degrees. Among them，SM was superior to SS in improving growth performance，immune indicators，and alleviating abnormal serum biochemical indicators. In summary，under high fluoride-induced oxidative stress conditions，adding SM to the diet can help alleviate the adverse effects of oxidative stress on the physiological functions of yellow-feathered broilers. Figures and Tables | References | Related Articles | Metrics

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Contamination characteristics and dietary exposure risk assessment of pentachlorophenol in shellfish from Ningbo JIANG Hai, ZHENG Dan, LU Ailan, WU Beili 2026, 67 (9):  2285-2289.  DOI: 10.16178/j.issn.0528-9017.20260442 Abstract ( 38 )   HTML ( 0 )   PDF (665KB) ( 141 )   To systematically and comprehensively understand the residue status of pentachlorophenol（PCP）in major commercial shellfish from Ningbo and to assess the health risk of dietary exposure to PCP via shellfish consumption，shellfish samples were collected from farming areas and markets in Ningbo from 2021 to 2025. The PCP concentrations in the samples were determined. Using the risk assessment model recommended by the US EPA，combined with population structure，body weight，and shellfish consumption，the estimated daily intake（EDI），hazard quotient（HQ）for non-carcinogenic risk，and cancer risk index（R）were calculated for different age groups to conduct exposure assessment. A total of 553 samples were analyzed. The overall mean PCP concentration over the five years was 0.94 μg·kg-1，with annual concentrations ranging from ND to 38.6 μg·kg-1，showing a decreasing trend. The mean concentration in razor clams（1.28 μg·kg-1）was slightly higher than the overall mean，while other species were at relatively low levels. The HQ for all age groups ranged from 7.0×10-5 to 9.4×10-5，all far below 1. R ranged from 1.4×10-7 to 1.9×10-7，below the lower bound of the US EPA acceptable risk range（10-6）. Among different shellfish species，razor clams made the highest contribution to HQ（45.4%），followed by oysters（35.5%）. The contamination level of pentachlorophenol in shellfish from Ningbo is generally low，and both non-carcinogenic and carcinogenic health risks from dietary exposure via shellfish consumption are within safe and controllable limits. It is recommended to continuously strengthen the supervision of farming environments and regulate the use of pond-cleaning chemicals to ensure the quality and safety of shellfish products. Figures and Tables | References | Related Articles | Metrics

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Black spot disease mediates nutritional metabolic disorders in Macrobrachium nipponense muscle QIU Qing, WANG Haizhi, WEI Leqiao, GUO Kefan, HUANG Fuyong, XU Guoming, YANG Qingman 2026, 67 (9):  2290-2293.  DOI: 10.16178/j.issn.0528-9017.20250602 Abstract ( 43 )   HTML ( 0 )   PDF (423KB) ( 140 )   To elucidate the mediating effect of black spot disease（BSD）on muscle nutritional metabolism and its associated health risks in Macrobrachium nipponense，this study systematically compared and analyzed conventional nutritional components，amino acid composition，fatty acid composition，and mineral elements content in diseased and healthy Macrobrachium nipponense. The results indicated that BSD induced extensive dysregulation of muscle nutritional metabolism. Compared with healthy Macrobrachium nipponense，the crude protein and crude ash content of muscles in diseased Macrobrachium nipponense were significantly（p<0.01）reduced. Total amounts of amino acids（TAA），essential amino acids（EAA，particularly threonine），semi-essential amino acids（SEAA），nonessential amino acids（NEAA），and delicious amino acid（DAA）were all significantly reduced. The contents of C16：0，C18：0，C14：1，C16：1，C17：1，and the total saturated fatty acids（SFA）significantly increased，while the contents of C20：0，C22：0，C18：1，C18：3 n-3，C20：5 n-3（eicosapentaenoic acid，EPA），C22：5 n-3，C22：6 n-3（docosahexaenoic acid，DHA），EPA+DHA，total polyunsaturated fatty acids（PUFA），and total n-3 PUFA significantly decreased. Furthermore，the contents of major elements（Na，K，Ca）and trace elements（Mn，Zn，Se）were significantly reduced. This study provides insights into the pathogenic mechanisms of BSD at the nutritional metabolic level and comprehensively reveals its detrimental effects on Macrobrachium nipponense health. The findings offer a scientific basis for developing targeted nutritional interventions and precise disease prevention strategies. Figures and Tables | References | Related Articles | Metrics

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Effects of different biodegradable mulching films on growth，yield and soil environment of taro CAI Meiyan, MAO Lingrong, LIN Guiping, LIN Yi, XIAO Wenduo 2026, 67 (9):  2294-2297.  DOI: 10.16178/j.issn.0528-9017.20250212 Abstract ( 39 )   HTML ( 0 )   PDF (383KB) ( 152 )   To study the effects of different biodegradable mulching films on the growth，yield and soil environment of taro，a comparative experiment was conducted on spring-sown taro using biodegradable film mulching，ordinary plastic film mulching and open-field cultivation. The growth of taro，soil environment and degradation of the mulching films were compared among different treatments. The results showed that biodegradable mulching films had significant soil warming effects and no significant influence on emergence rate and leaf age of taro. In terms of yield，Hongrui 8 performed best，being highly significantly（p<0.01）higher than the other treatments. Regarding degradation performance，Changfa 10 and Hongrui 8 degraded faster，while Jialemi 10 degraded slightly slower；by the time of taro harvest，the ridge surfaces of all biodegradable mulching film treatments had basically no film residue. In comprehensive comparison，biodegradable mulching films have certain advantages in taro production，as they can meet crop growth requirements and effectively control soil pollution，and thus can be promoted as substitutes for ordinary plastic films. Figures and Tables | References | Related Articles | Metrics

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Effect of different amounts of meal residue organic fertilizer on the growth of cabbage and soil improvement XU Haimin, YU Pengcheng, SHI Tongwu, XU Qiangqiang, CHEN Wei, YE Zhengqian 2026, 67 (9):  2298-2303.  DOI: 10.16178/j.issn.0528-9017.20250423 Abstract ( 34 )   HTML ( 1 )   PDF (466KB) ( 148 )   In order to evaluate the effect of meal residue organic fertilizer in vegetable production，cabbage was used as the test material，and no fertilization（CK），conventional chemical fertilization （CF），chemical fertilizer reduction+different amounts of commercial organic fertilizer or meal residue organic fertilizer were set up to analyze the effects of different amounts of commercial organic fertilizer or meal residue organic fertilizer on the growth of cabbage，soil physical and chemical properties，and nutrients content. The results showed that T6 treatment （chemical fertilizer reduction+15 t·hm-2 meal residue organic fertilizer） had the highest yield of cabbage，reaching 128.40 t·hm-2，which was significantly（p<0.05）increased by 652.20% compared with CK. There was no significant difference in the effect of meal residue organic fertilizer and commercial organic fertilizer on increasing the yield of cabbage. The pH value of the soil in the experimental area was alkaline，and compared with CK，the decrease in each fertilization treatment ranged from 3.72% to 7.69%. The soil electrical conductivity of T6 treatment was the highest，significantly increasing by 146.89% compared with CK. With the increase of amounts of meal residue organic fertilizer，soil electrical conductivity showed an upward trend. The T3 treatment （chemical fertilizer reduction+15 t·hm-2 commercial organic fertilizer） had the highest contents of soil organic matter，available phosphorus，and available potassium，which significantly increased by 41.64%，83.88%，and 75.80% compared with CK. The alkali-hydrolyzable nitrogen content of T6 treatment was the highest，significantly increasing by 15.51% compared with CK. The yield of cabbage was highly significantly（p<0.01）positively correlated with plant height，ball diameter，and soil available phosphorus and available potassium content， highly significantly negatively correlated with soil pH value，and significantly positively correlated with soil electrical conductivity. Based on comprehensive analysis， chemical fertilizer reduction+15 t·hm-2 meal residue organic fertilizer was most suitable for the growth and development of cabbage. Its fertility is good，providing a more suitable growth environment for cabbage and allowing for better resource utilization of meal residues. Figures and Tables | References | Related Articles | Metrics

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Effect of different amendments on coastal saline-alkali soil and sorghum growth YANG Jiaheng, MA Jiawei, WANG Mei, YANG Shaona 2026, 67 (9):  2304-2308.  DOI: 10.16178/j.issn.0528-9017.20250436 Abstract ( 39 )   HTML ( 0 )   PDF (591KB) ( 156 )   To investigate the effects of different soil amendments and application rates on the improvement of coastal saline-alkali soil in Zhoushan and their regulation on crop growth，sorghum was selected as the experimental material in coastal saline-alkali soil of Putuo District，Zhoushan City in 2023. Using no soil amendment as the control，three amendments，calcium sulfate，commercial organic fertilizer，and biochar，were employed to systematically compare their effects on the physicochemical properties of the saline-alkali soil and sorghum growth.The results showed that application of commercial organic fertilizers and biochar improved saline-alkali soil，with the T9 treatment（high application rate of biochar at 800 ke per 667 m2）performing the best. The T9 treatment resulted in the longest ear length，highest ear weight，and maximum thousand-kernel weight for sorghum，as well as the highest total nitrogen and phosphorus content in sorghum grains. Compared with CK，soil available phosphorus content were significantly（p<0.05）increased，indicating that high application rate of biochar was highly effective for improving coastal saline-alkali soil in Zhoushan. Figures and Tables | References | Related Articles | Metrics

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Effects of amendments and irrigation methods on salt leaching efficiency in clayey saline soil of southern Zhejiang YANG Jian, ZHANG Mingkui, WANG Liang 2026, 67 (9):  2309-2313.  DOI: 10.16178/j.issn.0528-9017.20250104 Abstract ( 39 )   HTML ( 1 )   PDF (377KB) ( 141 )   To explore rapid improvement techniques for coastal clayey saline soil，this study used indoor soil column leaching simulation to compare the effects of different irrigation methods（freshwater，calcium-containing freshwater，and alternating brackish water and freshwater）and soil application of desulfurization gypsum on salt leaching efficiency. The results showed that applying desulfurization gypsum to the soil accelerated the infiltration rate of irrigation water and promoted the leaching of water-soluble salts. Under the same soil treatment conditions，the salt removal efficiencies of the three irrigation methods were in the order：calcium-containing freshwater > alternating brackish water and freshwater > freshwater. Freshwater irrigation tended to cause soil alkalinization，whereas calcium-containing freshwater alleviated alkalinization；desulfurization gypsum application effectively inhibited alkalinization during the leaching process. In conclusion，using desulfurization gypsum for soil improvement combined with calcium-containing freshwater or alternating brackish water and freshwater irrigation can accelerate soil desalination and improve salt leaching efficiency. Figures and Tables | References | Related Articles | Metrics

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Effects of different organic materials on soil and yield of greenhouse vegetables QIAN Zhongming, HUANG Jiaqi, WU Lingen, WANG Ronglei, ZENG Xianjun, XU Lijun, CHEN Xiang 2026, 67 (9):  2314-2317.  DOI: 10.16178/j.issn.0528-9017.20250223 Abstract ( 37 )   HTML ( 0 )   PDF (359KB) ( 137 )   To address the problem that soil salinity accumulation in greenhouse vegetable production restricts the sustainable development of the vegetable industry，this study conducted a comparative experiment using four organic materials with contrasting C/N ratios，namely wood chips，rice straw，chicken manure，and pig manure. The results showed that all types of organic materials improved soil physical properties，with soil bulk density significantly（p<0.05）decreased by 3.73%-8.96%，the content of >0.25 mm water-stable aggregates in soil significantly increased by 14.98-20.19 percentage points，soil organic matter content significantly increased by 3.53%-5.11%，and total yield significantly increased by 11.73%-20.29%. These findings can provide a reference for the efficient，stable，and sustainable development of the greenhouse vegetable industry. Figures and Tables | References | Related Articles | Metrics

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Effects of combined application of soil conditioners and organic fertilizer on soil improvement and pepper yield in mountain CHEN Xiaoping, CAO Xuexian 2026, 67 (9):  2318-2322.  DOI: 10.16178/j.issn.0528-9017.20250351 Abstract ( 46 )   HTML ( 0 )   PDF (489KB) ( 140 )   To address the problem of soil acidification in mountain vegetable production，this study used organic fertilizer alone as the control and selected four widely used new soil conditioners，setting up five treatments to explore the effects of different conditioners combined with organic fertilizer on soil improvement and pepper yield. The results showed that T1（750 kg·hm-2 formula fertilizer + 15 000 kg·hm-2 organic fertilizer，combined with 1 500 kg·hm-2 soil conditioner 1）and T4（750 kg·hm-2 formula fertilizer +6 000 kg·hm-2 soil conditioner 4） treatments significantly （p<0.05）increased soil pH value. Compared with CK，pH value of T1 and T4 treatments increased by 0.55 and 0.38，respectively，and T1 treatment had the best effect. Compared with CK，T2 （750 kg·hm-2 formula fertilizer + 15 000 kg·hm-2 organic fertilizer，combined with 1 500 kg·hm-2 soil conditioner 2）and T4 treatments significantly increased soil available phosphorus content，and T4 treatment showed the best effect. In general， T1 and T4 treatments could maintain pepper yield，and effectively ameliorate acidic soil，thus serving as effective formulas for acidification improvement and sustained nutrient supply in acidic vegetable soils. Figures and Tables | References | Related Articles | Metrics

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Effect of biochar combined with Bacillus megaterium on secondary salinized soil and cucumber growth ZHENG Lingyan, HONG Qinghong, HUANG Wei, XING Chenghua 2026, 67 (9):  2323-2327.  DOI: 10.16178/j.issn.0528-9017.20250444 Abstract ( 39 )   HTML ( 0 )   PDF (447KB) ( 152 )   With the rapid development of greenhouse agriculture in China，secondary salinization has become increasingly severe，significantly affecting crop growth and soil health. This study investigated the effects of combining biochar with Bacillus megaterium on the remediation of secondary salinized soil. Through pot experiments，the effects of biochar combined with Bacillus megaterium on soil physicochemical properties，nitrate content，and cucumber growth were investigated. The results showed that treatments incorporating biochar and Bacillus megaterium（BT1 and BT2）significantly（p<0.05）reduced soil electrical conductivity（EC）and nitrate content. Specifically，the EC value of the soil under BT2 treatment was 47.25% lower than that of the control（CK），and nitrate content also decreased significantly. Additionally，the application of biochar and Bacillus megaterium significantly improved soil enzyme activity and microbial abundance. Simultaneously，the treatment of applying biochar and Bacillus megaterium significantly increased cucumber plant height，stem thickness，above-ground dry and fresh weight，and root vitality. The treatment also significantly enhanced the activities of antioxidant enzymes in cucumber leaves and reduced the content of malondialdehyde（MDA）. These findings indicated that the synergistic effect of biochar and Bacillus megaterium is highly effective in the remediation of secondary salinized soil，providing theoretical support and technical guidance for sustainable soil management in greenhouse agriculture. Figures and Tables | References | Related Articles | Metrics

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Field study on the regulation of paddy soil acidification and cadmium bioavailability by soil amendments HU Minjun, NI Kailun, ZHANG Haijuan, SHAO Sainan, DONG Daixing, MENG Qingjiu, ZHOU Chengyun, MENG Jun, CUI Zhonghua, XU Jun 2026, 67 (9):  2328-2333.  DOI: 10.16178/j.issn.0528-9017.20250207 Abstract ( 44 )   HTML ( 1 )   PDF (466KB) ( 143 )   Soil acidification exacerbates cadmium（Cd）activation and migration，posing a threat to the safe production of rice. This study conducted a field experiment in a Cd-contaminated paddy field in Zhejiang，China，to investigate the effects of five soil amendments：silicon-calcium-magnesium-potassium（SCMP），Ningliang shengwu，Fengshouyan，Tebei calcium，and lime，on ameliorating soil acidification and regulating Cd bioavailability. The results demonstrated that，except for SCMP，all other amendments significantly（p<0.05） increased soil pH value（from 0.50 to 1.28 ）and reduced the total exchangeable acid content by 74.68%-100.00%，with Fengshouyan showing the most pronounced effect. Additionally，all amendments significantly decreased bioavailable Cd content in soil and the Cd content in brown rice by 15.22%-58.94% and 17.04%-46.09%，respectively，among which Ningliang shengwu exhibited the optimal efficacy. In summary，Fengshouyan and Ningliang shengwu demonstrated superior performance in improving acidic soil and reducing Cd bioavailability，and their combined application was recommended. This study provided a scientific basis for the remediation of Cd-contaminated acidic paddy fields. Figures and Tables | References | Related Articles | Metrics

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Mechanisms and practical effects of the early rice-broccoli rotation model for common prosperity in Linhai City YE Junying, SHEN Ying, TANG Xuejun, CAI Jianjun, GAO Xu, ZHU Meihua 2026, 67 (9):  2334-2338.  DOI: 10.16178/j.issn.0528-9017.20250787 Abstract ( 51 )   HTML ( 4 )   PDF (412KB) ( 157 )   This paper takes the early rice-broccoli rotation model in Linhai City as the research object，focusing on how this model，through its core mechanism of "dual use of one field and double harvest of grain and cash crops"，effectively overcomes the continuous cropping obstacles of broccoli and alleviates the competition between grain and cash crops for land while improving land use efficiency and comprehensive benefits. The study reveals the internal logic and practical pathways through which this model achieves synergistic improvement of economic and ecological benefits，and analyzes its specific effects in ensuring regional food security，promoting agricultural efficiency and farmers' income growth，and advancing rural common prosperity，with a view to providing references for agricultural modernization and rural revitalization in similar regions. Figures and Tables | References | Related Articles | Metrics

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Structural characteristics of the spatial correlation network of agripreneurs in Zhejiang Province LI Xiaochu, XIAO Chenwen, LI Muwen, XIAO Wenxuan 2026, 67 (9):  2339-2344.  DOI: 10.16178/j.issn.0528-9017.20260489 Abstract ( 51 )   HTML ( 1 )   PDF (439KB) ( 135 )   This study focuses on agripreneurs in 64 counties（cities and districts）of Zhejiang Province as the research subjects. By constructing a comprehensive livelihood resilience evaluation index system（covering three dimensions：buffering capacity，self-organizing capacity，and learning capacity），the entropy method was employed to determine indicator weights. An improved gravity model was then used to establish the spatial network structure of agripreneurs. Using UCINET 6.0 and ArcGIS software，network density，centrality，and cohesive subgroups were analyzed. The findings reveal that the overall network connectivity density of agripreneurs in Zhejiang is relatively low（network density of 0.156 8），yet significant spatial clustering characteristics exist. Core regions such as Hangzhou and Huzhou emerge as network hubs due to resource aggregation and policy advantages，while geographical proximity and industrial complementarity serve as primary drivers of strong spatial associations. The research provides theoretical foundations and practical references for optimizing regional collaborative development policies for agripreneurs. Figures and Tables | References | Related Articles | Metrics