金柑CHS和CHI基因的克隆及表达分析

doi:10.16178/j.issn.0528-9017.20190744

摘要/Abstract

摘要： 为了研究查尔酮合成酶(chalcone synthase,CHS)和查尔酮异构酶(chalcone isomerase,CHI)在金柑类黄酮合成过程中的作用,从金柑果实中克隆3个CHS和2个CHI编码基因,构建进化树进行聚类分析;同时采用紫外分光法测定类黄酮含量,并通过qRT-PCR(quantitative real time polymerase chain reaction)进行定量分析。结果表明,FmCHS1和FmCHS2与已知功能CHS紧密聚为一类,界定为CHS蛋白;FmCHS3单独聚类,界定为CHS-like蛋白。FmCHI1界定为类型Ⅰ CHI蛋白,具有CHI酶催化活性,参与类黄酮合成;FmCHI2界定为类型Ⅳ CHI蛋白,在拟南芥中与类型Ⅰ CHI蛋白互作,促进类黄酮合成。在金柑果实发育过程中,类黄酮含量在果皮中呈明显的下降趋势,而在果肉中呈先升后降的变化趋势。FmCHS1和FmCHI1在金柑果实发育中呈高表达模式,且与类黄酮积累模式最为接近。FmCHS1和FmCHI1可能直接参与了金柑果实类黄酮的合成;虽然FmCHI2没有催化功能,但与FmCHI1互作促进类黄酮的合成。

关键词: 金柑, 类黄酮, 查尔酮合成酶, 查尔酮异构酶

中图分类号:

S666.1

马小迪, 谭嫣, 王久照, 刘小刚, 曾明. 金柑CHS和CHI基因的克隆及表达分析[J]. 浙江农业科学, 2019, 60(7): 1211-1215.

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