玄参中6种环烯醚萜和苯丙素类化合物含量检测及抗炎活性研究

doi:10.16178/j.issn.0528-9017.20250494

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (10): 2455-2461.DOI: 10.16178/j.issn.0528-9017.20250494

玄参中6种环烯醚萜和苯丙素类化合物含量检测及抗炎活性研究

张民达¹(), 李海申¹, 孙健¹^,²^,^*(), 王盼³, 邱萍⁴, 俞国良¹, 周潮滨¹, 王志安¹^,²

1.浙江省中药研究所有限公司,浙江杭州 310023
2.道地药材品质保障与资源持续利用全国重点实验室,北京 100700
3.磐安县中药创新发展研究院,浙江金华 322305
4.浙江中医药大学,浙江杭州 310053

收稿日期:2025-07-14 出版日期:2025-10-11 发布日期:2025-10-21
通讯作者: 孙健
作者简介:孙健,男,高级工程师,博士,研究方向为中药材抗性育种和生态栽培,E-mail:21116025@zju.edu.cn。
张民达(1995—),男,助理工程师,硕士,研究方向为中药材育种,E-mail:993612516@qq.com。
基金资助:
名贵中药资源可持续利用能力建设项目(2060302-2202-08);磐安县中药产业“揭榜挂帅”项目(PZYF202101);浙江省农业(中药材新品种选育)新品种选育重大科技专项(2021C02074);国家中医药管理局中医药科学技术研究专项课题(GZY-KJS-2021-020);浙江省中医药现代化专项项目(2022ZX003)

Detection of the contents of six iridoids and penylpropanoids in Scrophularia ninggpoensis Hemsl. and study on their anti-inflammatory activities

ZHANG Minda¹(), LI Haishen¹, SUN Jian¹^,²^,^*(), WANG Pan³, QIU Ping⁴, YU Guoliang¹, ZHOU Chaobin¹, WANG Zhian¹^,²

1. Zhejiang Institute of Chinese Materia Medica Co., Ltd., Hangzhou 310023, Zhejiang
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700
3. Pan'an County Institute of Traditional Chinese Medicine Innovation and Development, Jinhua 322305, Zhejiang
4. Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang

Received:2025-07-14 Online:2025-10-11 Published:2025-10-21
Contact: SUN Jian

摘要/Abstract

摘要： 本研究旨在分析玄参中6种环烯醚萜和苯丙素类化合物的含量分布规律,并探讨其与抗炎活性的相关性。采用高效液相色谱法测定了12批玄参样品中桃叶珊瑚苷、哈巴苷、毛蕊花糖苷、安格洛苷C、哈巴俄苷及肉桂酸的含量;同时利用RAW264.7细胞(小鼠单核巨噬细胞)模型评估不同玄参样品的抗炎活性。通过主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)、聚类分析及相关性分析,对样品的化学成分和抗炎活性进行系统解析,以筛选出关键的差异化合物及抗炎相关成分。结果显示,玄参样品中桃叶珊瑚苷的含量为0.80%~1.54%,哈巴苷的含量为0.65%~1.92%,毛蕊花糖苷的含量为0.05%~0.47%,安格洛苷C的含量为 0.25%~0.52%,哈巴俄苷的含量为0.19%~0.61%,肉桂酸的含量为0.01%~0.07%,不同样品的NO抑制率在22.33%~40.76%;主成分分析与偏最小二乘判别分析结果显示,不同样品间存在明显差异,主要差异化合物为肉桂酸、桃叶珊瑚苷和哈巴苷;聚类分析及相关性分析表明,哈巴苷和毛蕊花糖苷与抗炎活性呈正相关。通过对玄参活性成分及抗炎活性的测定,明确了其差异化合物及抗炎相关成分,为将苯丙素类化合物纳入玄参的质量控制提供了理论依据。

关键词: 玄参, 环烯醚萜, 苯丙素, 抗炎活性, 质量控制

Abstract:

This study aims to analyze the content distribution patterns of six components in Scrophularia ninggpoensis Hemsl. and explore their correlation with anti-inflammatory activity. The contents of aucubin, harpagide, verbascoside, angloside C, harpagoside and cinnamic acid in 12 batches of Scrophularia ninggpoensis Hemsl. samples were determined by high performance liquid chromatography. Meanwhile, the anti-inflammatory activity of different Scrophularia ninggpoensis Hemsl. samples was evaluated using the RAW264.7 cell (mouse mononuclear macrophage) model. Through principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), cluster analysis and correlation analysis, the chemical composition and anti-inflammatory activity of the samples were systematically analyzed to screen out the key differential compounds and their anti-inflammatory related components. The results showed that the content ranges of each component in Scrophularia ninggpoensis Hemsl. were as follows: the content of aucubin was 0.80% to 1.54%, the content of harpagide was 0.65% to 1.92%, the content of verbascoside was 0.05% to 0.47%, the content of angloside C was 0.25% to 0.52%, and the content of harpagoside was 0.19% to 0.61%. The content of cinnamic acid was 0.01% to 0.07%, and the NO inhibition rates of different samples ranged from 22.33% to 40.76%. The results of principal component analysis and partial least squares discriminant analysis showed that there were obvious differences among different samples, with the main differential compounds being cinnamic acid, aucubin and harpagide. Cluster analysis and correlation analysis indicated that harpagide and verbascoside were positively correlated with anti-inflammatory activity. Through the determination of the active components and anti-inflammatory activities of Scrophularia ninggpoensis Hemsl., the differential compounds and anti-inflammatory related components were identified, providing a theoretical basis for the inclusion of penylpropanoids in the quality control of Scrophularia ninggpoensis Hemsl..

Key words: Scrophularia ninggpoensis Hemsl., iridoids, penylpropanoids, anti-inflammatory activities, quality control

中图分类号:

S567

张民达, 李海申, 孙健, 王盼, 邱萍, 俞国良, 周潮滨, 王志安. 玄参中6种环烯醚萜和苯丙素类化合物含量检测及抗炎活性研究[J]. 浙江农业科学, 2025, 66(10): 2455-2461.

ZHANG Minda, LI Haishen, SUN Jian, WANG Pan, QIU Ping, YU Guoliang, ZHOU Chaobin, WANG Zhian. Detection of the contents of six iridoids and penylpropanoids in Scrophularia ninggpoensis Hemsl. and study on their anti-inflammatory activities[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2455-2461.

图/表 5

图1 玄参样品6种成分的HPLC含量

Fig.1 HPLC contents of six components in Scrophularia ninggpoensis Hemsl. samples

表1 不同浓度玄参提取液的细胞毒性

Table 1 Cytotoxicity of different concentrations of Scrophularia ninggpoensis Hemsl. extracts

浓度/(μg·mL^-1)	吸光度	相对标准偏差/%	细胞存活率/%
0	0.428 9	3.67	—
12.5	0.400 8	3.17	93.44
25.0	0.386 2	5.55	90.04
50.0	0.397 3	7.96	92.64
100.0	0.405 1	5.38	94.45
200.0	0.398 7	4.17	92.95
400.0	0.402 9	5.89	93.95

图2 不同玄参样品的抗炎活性

Fig.2 The anti-inflammatory activity of different samples of Scrophularia ninggpoensis Hemsl.

图3 不同玄参样品PCA及PLS-DA结果 A,玄参样品PCA结果;B,玄参样品PLS-DA结果

Fig.3 PCA and PLS-DA results of different Scrophularia ninggpoensis Hemsl. Samples

图4 不同玄参样品聚类热图及相关性分析结果 A,玄参样品聚类分析结果;B,玄参样品相关性分析结果; *、**代表在P<0.05和P<0.01水平显著、极显著相关。

Fig.4 Clustering heat maps and correlation analysis results of different Scrophularia ninggpoensis Hemsl. Samples

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玄参中6种环烯醚萜和苯丙素类化合物含量检测及抗炎活性研究

Detection of the contents of six iridoids and penylpropanoids in Scrophularia ninggpoensis Hemsl. and study on their anti-inflammatory activities

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