浙江省甘蓝链格孢引起的十字花科黑斑病菌危害寄主及分布范围调查

doi:10.16178/j.issn.0528-9017.20240761

浙江农业科学 ›› 2025, Vol. 66 ›› Issue (1): 146-149.DOI: 10.16178/j.issn.0528-9017.20240761

浙江省甘蓝链格孢引起的十字花科黑斑病菌危害寄主及分布范围调查

王玉玺¹(), 刘敏¹, 贾星语¹^,², 张震¹, 章金明¹^,^*()

1.浙江省农业科学院植物保护与微生物研究所, 浙江杭州 310021
2.安庆师范大学生命科学学院, 皖西南生物多样性研究与生态保护安徽省重点实验室, 安徽安庆 246133

收稿日期:2024-09-24 出版日期:2025-01-11 发布日期:2025-01-14
通讯作者: 章金明(1975—),男,浙江新昌人,副研究员,从事外来入侵物种和经济作物害虫监测预警及综合治理研究工作,E-mail:zhanginsect@163.com。
作者简介:王玉玺(1999—),女,河南郑州人,从事外来入侵物种监测预警相关工作,E-mail:wangyuxi0027@163.com。
基金资助:
浙江省“领雁”研发攻关计划项目(2022C02030);农业农村部农业外来入侵物种重点监测项目(13230120);农业农村部农业外来入侵物种重点监测项目(019240117)

Investigation of hosts and distribution of Alternaria brassicicola causing black spot of Cruciferae vegetables in Zhejiang Province

WANG Yuxi¹(), LIU Min¹, JIA Xingyu¹^,², ZHANG Zhen¹, ZHANG Jinming¹^,^*()

1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang
2. School of Life Sciences, Anqing Normal University, Anhui Provincial Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Anqing 246133, Anhui

Received:2024-09-24 Online:2025-01-11 Published:2025-01-14

摘要/Abstract

摘要：

十字花科黑斑病是一种世界性的重要真菌病害,通过种子传带形成远距离扩散和蔓延,可危害十字花科蔬菜的叶、茎、果等部位,造成严重的品质和产量损失。十字花科黑斑病有3种专化型致病菌,分别是甘蓝链格孢(Alternaria brassicicola),芸薹链格孢(Alternaria brassicae)和萝卜链格孢(Alternaria japonica)。中国南方十字花科蔬菜黑斑病多由甘蓝链格孢菌引起,为明确该菌引起的十字花科黑斑病在浙江省的危害状况,该研究利用分子鉴定方法,在全省开展了甘蓝链格孢菌危害寄主及分布范围调查。结果显示,5大类十字花科寄主植物中甘蓝链格孢菌的检获数量从高到低依次为白菜类>油菜类>芥菜类>甘蓝类>萝卜类。在全省11个地级市89个区县市中,有甘蓝链格孢菌分布的地级市有9个,占比81.8%;有甘蓝链格孢菌分布的县区市有27个,占比30.3%。甘蓝链格孢菌在浙江全省分布广泛,对众多寄主植物构成严重威胁,因此必须加强防治。

关键词: 十字花科黑斑病, 甘蓝链格孢, 寄主, 分布范围

Abstract:

Black spot is an important worldwide fungal disease in Cruciferae vegetables. It can spread far away through seed carrying, which can harm the leaves, stems, fruits and other parts of Cruciferae vegetables, causing serious quality and yield losses. Black spot is caused by three specialized pathogens, including Alternaria brassicicola, Alternaria brassicae, and Alternaria japonica. Black spot of Cruciferae vegetables in southern China is mostly caused by Alternaria brassicicola. In order to clarify the damage status of black spot in Zhejiang Province, this study used molecular identification techniques to investigate the infection host and distribution range of Alternaria brassicicola in the whole province. The results indicated the number of detected Alternaria brassicicola in the five major categories of Cruciferae host plants from high to low was Chinese cabbage> rapeseed> mustard>cabbage>radish. Among the 89 districts or counties in 11 prefecture-level cities, Alternaria brassicicola was detected in 27 districts or counties, accounting for 30.3%, and Alternaria brassicicola was detected in 9 cities, accounting for 81.8%. Alternaria brassicicola is widely distributed across Zhejiang Province and poses a significant threat to numerous host plants. Therefore, it is essential to strengthen control and prevention efforts.

Key words: black spot, Alternaria brassicicola, host, distribution area

中图分类号:

S436.35

王玉玺, 刘敏, 贾星语, 张震, 章金明. 浙江省甘蓝链格孢引起的十字花科黑斑病菌危害寄主及分布范围调查[J]. 浙江农业科学, 2025, 66(1): 146-149.

WANG Yuxi, LIU Min, JIA Xingyu, ZHANG Zhen, ZHANG Jinming. Investigation of hosts and distribution of Alternaria brassicicola causing black spot of Cruciferae vegetables in Zhejiang Province[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(1): 146-149.

图/表 5

图1 植物病样中甘蓝链格孢菌检测 1—Abra1/Abra 2引物扩增阳性对照;2~5—ZD1765-3~ZD1768-3的ITS1/ITS4引物扩增; 6~9—ZD1765-3~ZD1768-3的Abra1/Abra 2引物扩增;10—无菌水的Abra1/Abra2引物扩增。

Fig.1 Detection of Alternaria brassicicola in plant disease samples

图2 检获甘蓝链格孢菌的十字花科蔬菜类别占比

Fig.2 The detected proportion of Alternaria brassicicola in Cruciferae vegetable species

图3 甘蓝链格孢菌在浙江省11个地级市的分布概况此地图基于审图号为GS(2022)1873号的标准地图制作, 底图无修改,图4同。

Fig.3 Distribution of Alternaria brassicicola in 11 prefecture-level cities in Zhejiang Province

图4 甘蓝链格孢菌在浙江省89个区县市的分布概况

Fig.4 Distribution of Alternaria brassicicola in 89 districts, counties and cities of Zhejiang Province

图5 27个区县市甘蓝链格孢菌发生点位数

Fig.5 The occurrence points of A. brassicicola in 27 districts, counties and cities

参考文献 9

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浙江省甘蓝链格孢引起的十字花科黑斑病菌危害寄主及分布范围调查

Investigation of hosts and distribution of Alternaria brassicicola causing black spot of Cruciferae vegetables in Zhejiang Province

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