干湿交替条件下不同有机物料配施硝化抑制剂对稻田土壤氮素转化的影响

doi:10.16178/j.issn.0528-9017.20240563

摘要/Abstract

摘要：

采用微宇宙培养试验探究干湿交替条件下不同有机物料、硝化抑制剂及化学氮肥联合施用对稻田土壤氮素转化的影响。结果表明,干湿交替条件下3,4-二甲基吡唑磷酸盐(DMPP)可提高土壤铵态氮含量、抑制氨氧化细菌丰度,降低土壤净硝化速率。DMPP配施有机肥对提高土壤pH值及溶解有机碳和铵态氮含量的效果较佳。

关键词: 水稻土, 有机物料, 硝化抑制剂, 干湿交替, 氮素转化

Abstract:

Microcosmic culture experiments were used to investigate the effects of combined application of different organic materials, nitrification inhibitor and chemical nitrogen fertilizers on soil nitrogen transformation in paddy field under the condition of dry-wet alternation. It was found that under dry-wet alternation conditions, 3,4-dimethylpyrazole phosphate(DMPP) can increase soil ammonium nitrogen content, inhibit the abundance of ammonia-oxidizing bacteria, and reduce the soil net nitrification rate. Furthermore, the application of DMPP with organic fertilizer was more effective in increasing soil pH value, and content of dissolved organic carbon and DOC ammonium nitrogen content.

Key words: paddy soil, organic materials, nitrification inhibitor, dry-wet alternation, nitrogen conversion

中图分类号:

S146

生艳菲, 张闻汉, 张园园, 王峰, 傅丽青, 黄其颖, 卢忠诚, 叶楠, 卢九斤. 干湿交替条件下不同有机物料配施硝化抑制剂对稻田土壤氮素转化的影响[J]. 浙江农业科学, 2025, 66(10): 2324-2328.

SHENG Yanfei, ZHANG Wenhan, ZHANG Yuanyuan, WANG Feng, FU Liqing, HUANG Qiying, LU Zhongcheng, YE Nan, LU Jiujin. Effect of different organic materials combined with nitrification inhibitor on soil nitrogen conversion in paddy field under dry-wet alternation[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(10): 2324-2328.

图/表 7

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引物名称	引物序列(5'—3')	片段长度/bp
AOA-amoAF	STAATGGTCTGGCTTAGACG	635
AOA-amoAR	GCGGCCATCCATCTGGTATGT
AOB-amoAF	GGGGTTTCTACTGGTGGT	491
AOB-amoAR	CCCCTCKGSAAAGCCTTCTTC

引物名称	引物序列(5'—3')	片段长度/bp
AOA-amoAF	STAATGGTCTGGCTTAGACG	635
AOA-amoAR	GCGGCCATCCATCTGGTATGT
AOB-amoAF	GGGGTTTCTACTGGTGGT	491
AOB-amoAR	CCCCTCKGSAAAGCCTTCTTC

处理	不同处理时间(d)的土壤净硝化速率/ (mg·kg^-1·d^-1)
处理	7	14	21	28
CK	0.19 b	0.57 b	2.54 b	3.29 a
U	0.24 b	1.23 a	3.12 a	2.82 b
DMPP	0.09 c	-0.03 c	-0.15 d	0.31 d
BC	0.08 c	0.09 c	-0.28 d	0.79 c
S	0.17 b	-0.21 c	0.01 c	-0.01 e
M	1.44 a	-0.99 d	0.20 c	0.02 e

处理	不同处理时间(d)的土壤净硝化速率/ (mg·kg^-1·d^-1)
处理	7	14	21	28
CK	0.19 b	0.57 b	2.54 b	3.29 a
U	0.24 b	1.23 a	3.12 a	2.82 b
DMPP	0.09 c	-0.03 c	-0.15 d	0.31 d
BC	0.08 c	0.09 c	-0.28 d	0.79 c
S	0.17 b	-0.21 c	0.01 c	-0.01 e
M	1.44 a	-0.99 d	0.20 c	0.02 e

因子	DOC含量	pH值	AOB丰度	AOA丰度	$\text{NH} ^{+}_{4}$-N含量	$\text{NO} ^{-}_{3}$-N含量
pH值	0.886^**
AOB丰度	0.044	-0.032
AOA丰度	-0.380	-0.376	-0.311
$\text{NH} ^{+}_{4}$-N含量	0.335	0.572^**	-0.063	0.179
$\text{NO} ^{-}_{3}$-N含量	-0.234	-0.574^**	0.568^**	-0.061	-0.467^*
净硝化速率	-0.148	-0.500^*	0.627^**	-0.104	-0.502^*	0.972^**