丹参和藏丹参毛状根MYB和bHLH转录因子基因表达差异研究

doi:10.16178/j.issn.0528-9017.20190936

摘要/Abstract

摘要： 为了筛选参与丹参次生代谢的MYB和bHLH转录因子,选取47个MYB转录因子和47个bHLH转录因子基因进行分析。采用实时荧光定量PCR的方法,比较藏丹参和紫花丹参毛状根MYB和bHLH的基因表达差异。结果表明:4个MYB和6个bHLH在藏丹参中高表达,24个MYB和15个bHLH在丹参中高表达,19个MYB和26个bHLH在两种材料中表达差异不明显。这些差异表达基因可能与藏丹参高丹参酮和迷迭香酸积累以及低丹酚酸B积累有关。这为进一步研究藏丹参和紫花丹参次生代谢差异机制奠定了基础。

关键词: 丹参, 藏丹参, MYB转录因子, bHLH转录因子, 基因表达

中图分类号:

王艳婷, 郭妍宏, 王飞艳, 方誉民, 夏鹏国, 梁宗锁, 杨东风. 丹参和藏丹参毛状根MYB和bHLH转录因子基因表达差异研究[J]. 浙江农业科学, 2019, 60(9): 1593-1600.

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