Journal of Zhejiang Agricultural Sciences ›› 2024, Vol. 65 ›› Issue (2): 287-291.DOI: 10.16178/j.issn.0528-9017.20230068

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Effects of different cultivation conditions on cultivable microorganisms in wheat rhizosphere

Luqiong LYU1,3(), Younan OUYANG2, Shuzhen YE2, Yuxin YOU3, Bin LI3, Yanli WANG1,*()   

  1. 1. Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Zhejiang Provincial National Crop Variety Resistance Identification Test Station, Hangzhou 310021,Zhejiang
    2. China National Rice Research Institute, Hangzhou 310006,Zhejiang
    3. Institute of Biotechnology Research, Zhejiang University, Hangzhou 310058,Zhejiang
  • Received:2023-01-20 Online:2024-02-11 Published:2024-02-28
  • Contact: Yanli WANG

Abstract:

The rhizosphere microbial community of plants has a significant impact on their healthy growth, and isolating and cultivating their microorganisms is of great significance for promoting crop growth and yield increase. During the process of microbial cultivation, the type of culture medium and oxygenation capacity have different effects on the enrichment of rhizosphere microorganisms. This study used 16S rRNA high-throughput sequencing technology and bioinformatics analysis to analyze the characteristics and functions of wheat rhizosphere bacterial communities under different cultivation conditions. Research has shown that the microbial community in the rhizosphere of wheat is mainly composed of γ-Proteobacteria, Bacteroides, and Bacillus, and the type of culture medium and oxygen content significantly affect their abundance. The functional prediction of FAPROTAX indicated that the dominant microbial community in wheat rhizosphere microorganisms is mainly related to functions such as chemical heterotrophic, fermentation, carbon cycling, nitrogen cycling, and iron respiration, which played an important role in plant growth and development. The research results provided important basis for the cultivation of wheat rhizosphere microorganisms and the development and utilization of microbial resources.

Key words: rhizospheric microorganism, culture medium, oxygenation capacity, high throughput sequencing, microbial characteristics

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