Comparison of physicochemical properties of gardenia shell pectin extracted by three solutions

doi:10.16178/j.issn.0528-9017.20230247

Abstract

Abstract:

In order to study the effect of extraction solution on the physicochemical properties of gardenia shell pectin, gardenia shell pectin was extracted with citric acid, sodium hydroxide and water, respectively. The extraction yield, monosaccharide composition and molecular weight of pectin extracted by using different solutions were determined. The structural characteristics were compared by using Fourier transform infrared (FTIR), scanning electron microscopy and X-ray diffraction, and the antioxidant activity function was analyzed. The results showed that the yield of pectin from gardenia shell extracted by citric acid, water and sodium hydroxide was 17.20%, 16.93% and 16.67%, respectively. All of pectins were composed of 8 monosaccharides, and the molecular weight of pectin was water extracted pectin>alkali extracted pectin>acid extracted pectin; Three gardenia shell pectins showed a similar functional groups. According to the scanning electron microscope, the surface of gardenia shell pectin is rough and accompanied by micro-roll structure and particles, of which the alkali extracted has the smoothest surface. The X-ray diffraction results showed that all of pectins had semi-crystalline and amorphous structure. Pectin extracted by citric acid has the strongest DPPH scavenging ability, and that extracted by alkali is the weakest.

Key words: gardenia shell, pectin, physicochemical properties

CLC Number:

PAN Kang, XU Yuru, SHEN Weikang, ZHANG Zhi, XU Guangzhi. Comparison of physicochemical properties of gardenia shell pectin extracted by three solutions[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(6): 1482-1487.

Figures/Tables 8

References 27

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提取方法	单糖含量/%
提取方法	甘露糖	鼠李糖	葡萄糖醛酸	葡萄糖	半乳糖醛酸	半乳糖	木糖	阿拉伯糖
酸提	0.53±0.02 a	0.83±0.01 a	1.92±0.07 a	7.93±0.04 a	35.09±0.08 a	30.66±0.01 a	9.81±0.08 a	13.23±0.02 a
水提	0.61±0.01 a	0.80±0.06 a	1.91±0.01 a	7.22±0.02 c	36.03±0.02 b	30.65±0.01 a	9.80±0.05 a	12.98±0.03 b
碱提	0.55±0.00 a	0.79±0.06 a	1.91±0.08 a	7.41±0.02 b	36.34±0.07 b	30.63±0.00 a	9.38±0.04 b	12.99±0.03 b

提取方法	单糖含量/%
提取方法	甘露糖	鼠李糖	葡萄糖醛酸	葡萄糖	半乳糖醛酸	半乳糖	木糖	阿拉伯糖
酸提	0.53±0.02 a	0.83±0.01 a	1.92±0.07 a	7.93±0.04 a	35.09±0.08 a	30.66±0.01 a	9.81±0.08 a	13.23±0.02 a
水提	0.61±0.01 a	0.80±0.06 a	1.91±0.01 a	7.22±0.02 c	36.03±0.02 b	30.65±0.01 a	9.80±0.05 a	12.98±0.03 b
碱提	0.55±0.00 a	0.79±0.06 a	1.91±0.08 a	7.41±0.02 b	36.34±0.07 b	30.63±0.00 a	9.38±0.04 b	12.99±0.03 b

提取方法	Mw/ku	Mn/ku	PDI
酸提	495.5±35.1 b	283.7±19.2 b	1.74±0.08 a
水提	504.3±15.8 a	328.9±11.4 a	1.53±0.11 b
碱提	484.8±31.0 b	311.5±9.2 a	1.55±0.15 b

提取方法	Mw/ku	Mn/ku	PDI
酸提	495.5±35.1 b	283.7±19.2 b	1.74±0.08 a
水提	504.3±15.8 a	328.9±11.4 a	1.53±0.11 b
碱提	484.8±31.0 b	311.5±9.2 a	1.55±0.15 b

提取方法	回归方程	R²	IC₅₀/ (mg·mL^-1)	总酚含量/ (mg·g^-1)
酸提	Y=28.5X+2.23	0.994	1.67±0.11 a	1.97±0.12 b
水提	Y=29.31X-0.17	0.992	1.71±0.08 a	1.90±0.14 b
碱提	Y=23.2X+5.79	0.995	1.90±0.09 b	1.58±0.09 a