Abstract:

A method for rapid determination of pentachlorophenol residues in honey by HPLC-MS/MS was established. The sample was extracted by ammoniated ethyl acetate (9∶1, V∶V), concentrated and determined by liquid chromatography tandem mass spectrometry, and quantified by internal standard method. Under the optimal conditions, the recovery ranged from 93.5% to 101.3% at the three spiked levels (0.5, 2.0 and 10.0 μg·kg^-1) and the relative standard deviations ranged from 4.30% to 6.82%. The limits of detection and quantification were 0.2 and 0.5 μg·kg^-1, respectively. This method is simple, fast, and reliable, and can be used to determine pentachlorophenol residues in honey.

Key words: HPLC/MS/MS, pentachlorophenol, honey