Abstract:

In this paper, a method for the determination of lycopene content in tomato by ultra-high performance liquid chromatography (UPLC) was established. 10 mL of acetone was added to the 2.00 g sample, mixed with a whirl instrument for 10 min, centrifuged at 4 ℃ at 10 000 r·min^-1 for 5 min, and the supernatant was passed through a 0.22 μm microporous filter membrane for detection. The results showed that lycopene showed a good linear relationship in the concentration range of 1.00-100.00 μg·mL^-1, and the recovery rate was 91.2%-106.7%, and the relative standard deviation (RSD) was 2.8%-5.1%. This method can save a lot of time and reagents, and make the pretreatment simpler, more economical, and more environmentally friendly.

Key words: lycopene, ultra-high performance liquid chromatography, tomato