Molecular cloning, bioinformatics and expression analysis of vacuolar invertase inhibitor in tomato

doi:10.16178/j.issn.0528-9017.20230461

Abstract

Abstract:

Vacuolar invertase inhibitor (VIF) plays an important role in regulating higher plant sucrose metabolism and responding to abiotic stress. In this study, VIF gene and cDNA sequence were cloned from Alisa. The results indicated that the full length of SlVIF gene and cDNA was 528 bp, without intron region, encoding 175 amino acids. The molecular weight of SlVIF protein was 19.92 ku, and the theoretical isoelectric point was 5.53. It contained a signal peptide sequence, and the conservative domain was PMEI. The phylogenetic relationship exhibited that the VIF of Solanaceae species was the closest and the motifs were consistent. The tissue specific expression results showed that it had different expression patterns in various tomato materials. In Heinz, it had high transcription level in roots. In LA1589, it was highly expressed in all stages of fruit development, especially in mature fruits and roots, which indicated that SlVIF gene may play a role in influencing fruit ripening and regulating root growth by mediating sucrose metabolism. SlVIF was induced by water stress and responded to abiotic stress processes.

Key words: vacuolar invertase inhibitor, tomato, sucrose metabolism, expression

CLC Number:

S641.2

XU Yang, CHENG Yuan, RUAN Meiying, WANG Rongqing, YE Qingjing, YAO Zhuping, ZHOU Guozhi, LIU Lecheng, WAN Hongjian. Molecular cloning, bioinformatics and expression analysis of vacuolar invertase inhibitor in tomato[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(1): 81-89.

Figures/Tables 8

Table 1 SlVIF gene primer information

引物名称	序列(5'-3')
SlVIF-F	ATGAGAAATTTATTCCCCATATTGATG
SlVIF-R	TCATAATAACATTCTAATTATGGATTTAGC

Fig.1 Cloning of tomato SlVIF gene

Fig.2 SlVIF protein sequence analysis

Fig.3 Structure prediction of SlVIF protein

Fig.4 Prediction of the conservative domain of SlVIF protein

Fig.5 Phylogenetic relationship and conserved motif analysis of plant vacuolar sucrose invertase inhibitory protein system

Fig.6 Heat map of the expression pattern of SlVIF gene in different tissues and organs of tomato

Fig.7 Heat map of expression patterns of SlVIF gene under different abiotic stresses

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