Effect of exogenous salicylic acid on the photosynthetic and physiological characteristics of Viola tricolor L. under cadmium stress

doi:10.16178/j.issn.0528-9017.20240044

Abstract

Abstract:

This article explored the effects of cadmium stress and exogenous salicylic acid on the photosynthesis and physiological characteristics of Viola tricolor L., providing a basis for rational application of salicylic acid and alleviating cadmium toxicity. Viola tricolor L. was planted under Cd stress (200 mg·kg^-1), and spray 0 (SA0) and 50 (SA1), 100 (SA2), 200 (SA3), 500 (SA4) μmol·L^-1 salicylic acid on the leaves, to explore its effects on the growth, photosynthesis, osmoregulation substances and enzymatic protection system of Viola tricolor L. The results showed that 200 mg·kg^-1 of Cd can lead to a significant increase in MDA, significantly inhibit the vegetative growth, reproductive growth and photosynthesis of Viola tricolor L.; The application of an appropriate amount of salicylic acid (100-200 μmol·L^-1) can reduce the content of MDA and increase the plant length, stem diameter, leaf area, number of leaves and flowers, dry weight, fresh weight and water content of Viola tricolor L. under Cd stress; The net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r) were increased; The application of an appropriate amount of salicylic acid (100-200 μmol·L^-1) can also increase the content of proline, soluble protein, soluble sugar and the activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), but if the concentration reaches 500 μmol·L^-1, the effect is not as good as when the concentration is lower, indicating that an appropriate amount of salicylic acid (100-200 μmol·L^-1) can significantly alleviate the Cd toxicity of Viola tricolor L., and 200 μmol·L^-1 is the best application concentration based on all indicators.

Key words: Viola tricolor L., salicylic acid, cadmium stress, photosynthetic parameters

CLC Number:

S682

GONG Hongjie, JI Kangxuan, LI Zhu, WANG Bo. Effect of exogenous salicylic acid on the photosynthetic and physiological characteristics of Viola tricolor L. under cadmium stress[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(6): 1416-1423.

Figures/Tables 8

Table 1 Settings of experimental treatment

处理	Cd浓度/(mg·kg^-1)	SA浓度/(μmol·L^-1)
CK	0	0
SA0	200	0
SA1	200	50
SA2	200	100
SA3	200	200
SA4	200	500

Fig.1 Effect of salicylic acid on the biomass of Viola tricolor L. under cadmium stress

Table 2 Effects of salicylic acid on growth indexes of Viola tricolor L. under cadmium stress

处理	株高/cm	茎粗/mm	叶面积/cm²	叶片数	开花数
CK	10.10±1.07 a	2.70±0.37 a	9.89±1.59 a	15.90±2.47 a	4.70±1.58 a
SA0	6.33±0.61 d	1.37±0.26 d	5.88±1.44 c	10.44±3.05 c	1.33±1.00 c
SA1	7.06±0.56 c	1.50±0.22 d	7.44±0.96 b	10.56±3.05 bc	1.44±0.88 c
SA2	7.32±0.53 bc	1.98±0.24 c	8.14±1.13 b	11.22±1.99 bc	2.44±1.13 b
SA3	7.83±0.48 b	2.19±0.21 b	8.42±1.05 b	13.22±1.86 b	2.78±0.97 b
SA4	7.19±0.55 c	1.83±0.22 c	6.56±1.00 c	11.11±1.76 bc	2.00±0.87 b

Fig.2 Effect of salicylic acid on the SPAD value of Viola tricolor L. under cadmium stress

Fig.3 Effect of salicylic acid on photosynthetic parameters of Viola tricolor L. under cadmium stress

Fig.4 Effect of salicylic acid on MDA content of Viola tricolor L. under cadmium stress

Fig.5 Effect of salicylic acid on osmoregulation substances content of Viola tricolor L. under cadmium stress

Fig.6 Effect of salicylic acid on enzymatic protection system of Viola tricolor L. under cadmium stress

References 24

[1]	SANDALIO L M, DALURZO H C, GÓMEZ M, et al. Cadmium-induced changes in the growth and oxidative metabolism of pea plants[J]. Journal of Experimental Botany, 2001, 52(364): 2115-2126.
[2]	沈建伟, 徐加宽, 刘建国, 等. 苏州市农田土壤重金属污染状况研究[J]. 环境科学与技术, 2010, 33(S2): 87-89, 93.
[3]	AHMAD P, NABI G, ASHRAF M. Cadmium-induced oxidative damage in mustard[Brassica juncea (L.) Czern.& Coss.]plants can be alleviated by salicylic acid[J]. South African Journal of Botany, 2011, 77(1): 36-44.
[4]	郑宏春, 刘姣, 王涵, 等. 植物细胞吸收镉的机理及镉对植物气孔发育的影响[J]. 地球环境学报, 2019, 10(5): 496-502.
[5]	潘瑞炽. 植物生理学[M]. 7版. 北京: 高等教育出版社,2012: 226-227.
[6]	贾茵, 刘才磊, 兰晓悦, 等. 外源水杨酸对镉胁迫下小报春幼苗生长及生理特性的影响[J]. 草地学报, 2020, 28(5): 1346-1354.
[7]	张爱慧, 朱士农. 外源水杨酸对盐胁迫下丝瓜幼苗生长和抗氧化酶活性的影响[J]. 江西农业学报, 2013, 25(10): 27-29.
[8]	姚岭柏, 任琴, 韩海霞. 外源水杨酸对干旱胁迫下马铃薯生长及抗性生理指标的影响[J]. 江苏农业科学, 2017, 45(24): 68-72.
[9]	GU C S, YANG Y H, SHAO Y F, et al. The effects of exogenous salicylic acid on alleviating cadmium toxicity in Nymphaea tetragona Georgi[J]. South African Journal of Botany, 2018, 114: 267-271.
[10]	张翔, 杨勇, 刘学勇, 等. 外源水杨酸对低温胁迫下海滨雀稗抗寒生理特征的影响[J]. 草业学报, 2020, 29(1): 117-124.
[11]	包满珠. 花卉学[M]. 2版. 北京: 中国农业出版社,2003: 179-181.
[12]	张其生, 包满珠, 卢兴霞, 等. 大花三色堇育种研究进展[J]. 植物学报, 2010, 45(1): 128-133.
[13]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[14]	KOVÁČIK J, GRÚZ J, BAČKOR M, et al. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants[J]. Plant Cell Reports, 2009, 28(1): 135-143.
[15]	王小红, 郭军康, 贾红磊, 等. 外源水杨酸缓解镉对番茄毒害作用的研究[J]. 农业环境科学学报, 2019, 38(12): 2705-2714.
[16]	李雷, 刘彤, 邓群仙, 等. 外源水杨酸对镉污染下酸枣幼苗生长及镉积累的影响[J]. 四川农业大学学报, 2019, 37(3):359-365, 403.
[17]	BELKHADI A, HEDIJI H, ABBES Z, et al. Effects of exogenous salicylic acid pre-treatment on cadmium toxicity and leaf lipid content in Linum usitatissimum L[J]. Ecotoxicology and Environmental Safety, 2010, 73(5): 1004-1011.
[18]	张金彪, 黄维南. 镉胁迫对草莓光合的影响[J]. 应用生态学报, 2007, 18(7): 1673-1676.
[19]	HAYYAN M, HASHIM M A, ALNASHEF I M. Superoxide ion: generation and chemical implications[J]. Chemical Reviews, 2016, 116(5): 3029-3085.
[20]	刘伟, 樊文华, 刘奋武, 等. 施磷对镉胁迫下黄瓜苗期光合作用及抗氧化酶系统的影响[J]. 土壤通报, 2022, 53(3): 596-604.
[21]	韩鹰, 邓鹏, 陈刚. 外源水杨酸改善Cd胁迫下路易斯安娜鸢尾的生长和养分吸收[J]. 应用生态学报, 2015, 26(10): 3153-3159.
[22]	潘瑞炽. 植物生理学[M]. 7版. 北京: 高等教育出版社, 2012: 320-323.
[23]	郭春芳, 孙云. 干旱胁迫下植物的渗透调节及脯氨酸代谢研究进展[J]. 福建教育学院学报, 2015, 16(1): 114-118, 128.
[24]	杨张钰, 朱心雨, 胡乐佳, 等. 外源水杨酸对镉胁迫下藜麦幼苗生长生理的缓解效应[J]. 江苏农业科学, 2022, 50(4): 52-57.