Effect of citric acid on rhizosphere microorganisms of flax in cadmium-contaminated soil

doi:10.16178/j.issn.0528-9017.20240161

Abstract

Abstract:

In this study, the rhizosphere soil was taken at the physiological maturity stage of flax, and the effects of different concentrations of citric acid on the diversity, abundance and community structure of bacteria in the rhizosphere soil of flax were analyzed by 16S rRNA gene high-throughput sequencing technology and bioinformatics methods. The results showed that the ACE, Chao1, Simpson and Shannon index values of bacteria in the rhizosphere soil of flax treated with high concentration of citric acid were the highest, and the ACE and Chao1 index values were significantly higher than the control. The results of PCoA analysis showed that the community structure of bacterial OTU in the rhizosphere soil of flax treated with high concentration citric acid was the most different from that in the control soil. In terms of bacterial composition, all the dominant bacteria phylum was Patescibacteria, with an average proportion of 49.50%, and all the dominant bacteria genus was TM7a, with an average proportion of 32.99%. This experiment is helpful to provide a theoretical reference for the subsequent study of the effect of exogenous organic acids on the absorption of cadmium in flax.

Key words: citric acid, flax, rhizosphere soil, bacteria

CLC Number:

LIU Tingting, LI Wenlue, LUO Xiahong, ZOU Lina, CHEN Changli, ZHU Guanlin, AN Xia. Effect of citric acid on rhizosphere microorganisms of flax in cadmium-contaminated soil[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(5): 1145-1150.

Figures/Tables 5

References 16

[1]	任泺彤, 陈小光, 马颜雪, 等. 麻文化发展与高值利用前景展望[J]. 中国麻业科学, 2021, 43(5): 272-280.
[2]	柳婷婷, 骆霞虹, 李文略, 等. 不同亚麻品种重金属Cd吸收积累的差异[J]. 浙江农业科学, 2022, 63(9): 2030-2032, 2045.
[3]	刘丽丽, 李建辉, 郑雪良, 等. 早实薄皮核桃根际与非根际土壤微生物多样性分析[J]. 浙江农业科学, 2022, 63(7): 1487-1489.
[4]	胡群群, 李志安, 黄宏星, 等. 柠檬酸促进土壤镉解吸的机理研究[J]. 生态环境学报, 2011, 20(S2): 1338-1342.
[5]	詹淑威, 潘伟斌, 赖彩秀, 等. 外源有机酸对小飞扬草(Euphorbia thymifolia L.)修复镉污染土壤的影响[J]. 环境工程学报, 2015, 9(10): 5096-5102.
[6]	薛博晗, 李娜, 宋桂龙, 等. 外源柠檬酸、苹果酸和草酸对披碱草镉耐受及富集的影响[J]. 草业学报, 2018, 27(6): 128-136.
[7]	陈红纯, 吴科君, 李瑞, 等. 外源有机酸对镉胁迫下秋华柳镉积累特征的影响[J]. 生态学报, 2019, 39(12): 4510-4518.
[8]	杨波, 陈银萍, 柯昀琪, 等. EDDS对Cd胁迫下三叶鬼针草生长和抗氧化酶系统及Cd积累的影响[J]. 农业环境科学学报, 2018, 37(5): 875-882.
[9]	GUO M J, WU F H, HAO G G, et al. Bacillus subtilis improves immunity and disease resistance in rabbits[J]. Frontiers in Immunology, 2017, 8: 354.
[10]	邹丽娜, 柳婷婷, 李文略, 等. 麻地膜覆盖对番茄根际土壤微生物群落结构的影响[J]. 浙江农业科学, 2023, 64(2): 371-378.
[11]	JONES D L, DENNIS P G, OWEN A G, et al. Organic acid behavior in soils-misconceptions and knowledge gaps[J]. Plant and Soil, 2003, 248(1): 31-41.
[12]	丁永祯, 李志安, 邹碧. 土壤低分子量有机酸及其生态功能[J]. 土壤, 2005, 37(3): 243-250.
[13]	李华英. 外源柠檬酸和草酸对镉胁迫下苎麻生理响应的影响研究[D]. 长沙: 湖南大学, 2014.
[14]	郭晖, 庄静静. 外源柠檬酸对3种观赏植物修复铅污染土壤的影响[J]. 西南农业学报, 2021, 34(1): 137-143.
[15]	姚琴, 杨爱江, 牛阿萍, 等. 两种外源有机酸对蜈蚣草修复锑污染土壤的影响[J]. 环境科学学报, 2022, 42(8): 405-414.
[16]	ONEN O I, ABOH A A, MFAM A N, et al. Microbial diversity: values and roles in ecosystems[J]. Asian Journal of Biology, 2020: 10-22.

处理	覆盖度/%	ACE指数	Chao1指数	Shannon指数	Simpson指数
CK	99.258 0	2 173.902 9±90.233 5 b	2 055.474 0±100.321 3 b	5.992 8±0.259 4 a	0.922 2±0.005 7 a
CA1	99.311 1	2 227.869 6±181.434 5 ab	2 114.318 7±172.826 1 ab	6.226 1±0.821 3 a	0.910 0±0.043 5 a
CA2	99.322 9	2 045.334 9±127.021 4 b	1 925.964 1±140.228 0 b	5.885 4±0.863 8 a	0.900 1±0.051 9 a
CA3	99.211 3	2 423.346 4±37.131 2 a	2 298.923 0±29.858 1 a	7.011 8±0.132 7 a	0.956 0±0.005 7 a

处理	覆盖度/%	ACE指数	Chao1指数	Shannon指数	Simpson指数
CK	99.258 0	2 173.902 9±90.233 5 b	2 055.474 0±100.321 3 b	5.992 8±0.259 4 a	0.922 2±0.005 7 a
CA1	99.311 1	2 227.869 6±181.434 5 ab	2 114.318 7±172.826 1 ab	6.226 1±0.821 3 a	0.910 0±0.043 5 a
CA2	99.322 9	2 045.334 9±127.021 4 b	1 925.964 1±140.228 0 b	5.885 4±0.863 8 a	0.900 1±0.051 9 a
CA3	99.211 3	2 423.346 4±37.131 2 a	2 298.923 0±29.858 1 a	7.011 8±0.132 7 a	0.956 0±0.005 7 a