Profile analysis of soil bacterial community distribution and nitrogen metabolism in citrus orchard under combined application of animal and poultry organic fertilizer and chemical fertilizer

doi:10.16178/j.issn.0528-9017.20240838

Abstract

Abstract:

In order to investigate the changes in the distribution and function of bacterial communities in the soil profiles of citrus under the combined application of animal and poultry organic fertilizers and chemical fertilizers, a typical citrus orchard in Quzhou City was selected as the research sample. The soil physicochemical properties of two varieties of citrus, Penggan and Xingjin, were analyzed under different profiles 0-20 cm(≤20 cm)and >20-40 cm. The results showed that soil pH value in 0-20 cm soil layer was significantly lower than that in >20-40 cm soil layer(P<0.05), and the contents of total nitrogen(TN), total phosphorus(TP), microbial biomass carbon(MBC), microbial biomass nitrogen(MBN) and microbial biomass phosphorus(MBP) in >20-40 cm soil layer were significantly higher than those in >20-40 cm soil layer(P<0.05). The results of bacterial community analysis showed that the diversity and abundance of bacteria in 0-20 cm soil layer were higher than those in >20-40 cm soil layer. The dominant bacterial phyla phyla in the soil layer were Pseudomonadota, Acidobacteriota and Actinomycetota. In addition, the activities of enzymes related to nitrogen metabolism were higher in shallow soil(0-20 cm). Correlation analysis showed that Acidobacterium, Actinophytocola and other dominant bacterial phyla were significantly positively correlated with TN, TP, MBC, MBN and MBP contents(P<0.05), and significantly negatively correlated with pH value contents(P<0.05). In general, compared with conventional fertilization, the combination of animal and poultry organic fertilizer and chemical fertilizer optimized the physical and chemical properties of 0-20 cm soil layer, improved the bacterial diversity and functional activity of 0-20 cm soil layer in citrus orchard, but had little effect on >20-40 cm soil layer.

Key words: animal and poultry organic fertilizer, chemical fertilizer, citrus orchard soil bacterial community, microbial diversity, nitrogen metabolism

CLC Number:

S154.3

TONG Wenbin, ZHENG Zehua, YANG Haijun, LI Ronghui, YE Zhengqian. Profile analysis of soil bacterial community distribution and nitrogen metabolism in citrus orchard under combined application of animal and poultry organic fertilizer and chemical fertilizer[J]. Journal of Zhejiang Agricultural Sciences, 2025, 66(3): 760-768.

Figures/Tables 5

References 42

[1]	AL-RIMAWI F, HIJAZ F, NEHELA Y, et al. Uptake, translocation, and stability of oxytetracycline and streptomycin in Citrus plants[J]. Antibiotics, 2019, 8(4): 196.
[2]	杨青松, 杨伟, 彭珏, 等. 典型黑土区坡耕地土壤微生物多样性及群落结构对侵蚀-沉积的响应[J]. 土壤学报, 2024, 61(6):1741-1754..
[3]	CUSTÓDIO V, GONIN M, STABL G, et al. Sculpting the soil microbiota[J]. Plant Journal, 2022, 109(3): 508-522.
[4]	CHEN W, LI Z W, SHEN X. Influence of soil acidification on soil microorganisms in pear orchards[J]. Communications in Soil Science and Plant Analysis, 2012, 43(13): 1833-1846.
[5]	BEHNKE G D, KIM N, ZABALOY M C, et al. Soil microbial indicators within rotations and tillage systems[J]. Microorganisms, 2021, 9(6): 1244.
[6]	FIERER N, WOOD S A, BUENO DE MESQUITA C P. How microbes can, and cannot, be used to assess soil health[J]. Soil Biology and Biochemistry, 2021, 153: 108111.
[7]	ASTUDILLO-GARCÍA C, HERMANS S M, STEVENSON B, et al. Microbial assemblages and bioindicators as proxies for ecosystem health status: potential and limitations[J]. Applied Microbiology and Biotechnology, 2019, 103(16): 6407-6421.
[8]	BICKEL S, OR D. Soil bacterial diversity mediated by microscale aqueous-phase processes across biomes[J]. Nature Communications, 2020, 11(1): 116.
[9]	JIN Y B, FANG Z, ZHOU X B. Variation of soil bacterial communities in a chronosequence of citrus orchard[J]. Annals of Microbiology, 2022, 72(1): 21.
[10]	王慧颖, 徐明岗, 周宝库, 等. 黑土细菌及真菌群落对长期施肥响应的差异及其驱动因素[J]. 中国农业科学, 2018, 51(5): 914-925.
[11]	YANG Y D, WANG P X, ZENG Z H. Dynamics of bacterial communities in a 30-year fertilized paddy field under different organic-inorganic fertilization strategies[J]. Agronomy, 2019, 9(1): 14.
[12]	FAN M C, LI J J, YAN W M, et al. Shifts in the structure and function of wheat root-associated bacterial communities in response to long-term nitrogen addition in an agricultural ecosystem[J]. Applied Soil Ecology, 2021, 159: 103852.
[13]	LI Y, NIE C, LIU Y H, et al. Soil microbial community composition closely associates with specific enzyme activities and soil carbon chemistry in a long-term nitrogen fertilized grassland[J]. Science of the Total Environment, 2019, 654: 264-274.
[14]	THAKUR A, SHARMA R P, SANKHYAN N K, et al. Effect of 46 years' application of fertilizers, FYM and lime on physical, chemical and biological properties of soil under maize-wheat system in an acid Alfisol of northwest Himalayas[J]. Soil Use and Management, 2023, 39(1): 357-367.
[15]	JOSEPH B, BABU S. Effect of organic and chemical fertilizer on the diversity of rhizosphere and leaf microbial composition in sunflower plant[J]. Current Microbiology, 2024, 81(10): 331.
[16]	REN J H, LIU X L, YANG W P, et al. Rhizosphere soil properties, microbial community, and enzyme activities: short-term responses to partial substitution of chemical fertilizer with organic manure[J]. Journal of Environmental Management, 2021, 299: 113650.
[17]	WANG J, CUI W L, CHE Z, et al. Effects of synthetic nitrogen fertilizer and manure on fungal and bacterial contributions to N₂O production along a soil acidity gradient[J]. Science of the Total Environment, 2021, 753: 142011.
[18]	WANG J, SUN N, XU M G, et al. The influence of long-term animal manure and crop residue application on abiotic and biotic N immobilization in an acidified agricultural soil[J]. Geoderma, 2019, 337: 710-717.
[19]	LIU Z P, XIE W Y, YANG Z X, et al. Effects of manure and chemical fertilizer on bacterial community structure and soil enzyme activities in north China[J]. Agronomy, 2021, 11(5): 1017.
[20]	LAI K Y, XIAO J C, WAN X F, et al. Effect of combined application of organic and chemical fertilizers on yield and quality of epimedium pubescens[J]. China Journal of Chinese Materia Medica, 2024, 49(11): 2871-2881.
[21]	IQBAL A, ALI I, YUAN P L, et al. Combined application of manure and chemical fertilizers alters soil environmental variables and improves soil fungal community composition and rice grain yield[J]. Frontiers in Microbiology, 2022, 13: 856355.
[22]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[23]	VANCE E D, BROOKES P C, JENKINSON D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry, 1987, 19(6): 703-707.
[24]	BROOKES P C, POWLSON D S, JENKINSON D S. Measurement of microbial biomass phosphorus in soil[J]. Soil Biology and Biochemistry, 1982, 14(4): 319-329.
[25]	王洪杰, 李宪文, 史学正, 等. 不同土地利用方式下土壤养分的分布及其与土壤颗粒组成关系[J]. 水土保持学报, 2003(2): 44-46, 50.
[26]	李龙, 秦富仓, 姜丽娜, 等. 区县域尺度土壤全氮的空间分布格局分析[J]. 生态学报, 2020, 40(5): 1572-1579.
[27]	LYNGSIE G, PENN C J, HANSEN H C B, et al. Phosphate sorption by three potential filter materials as assessed by isothermal titration calorimetry[J]. Journal of Environmental Management, 2014, 143: 26-33.
[28]	张田, 许浩, 茹淑华, 等. 不同有机肥中磷在土壤剖面中累积迁移特征与有效性差异[J]. 环境科学, 2017, 38(12): 5247-5255.
[29]	刘珊珊. 庐山土壤有机质含量分析[J]. 农业开发与装备, 2017(11): 86.
[30]	马利芳, 熊黑钢, 王宁, 等. 不同深度土壤盐分和有机质含量的空间变异特征[J]. 江苏农业科学, 2019, 47(16): 264-270.
[31]	HAO J J, CHAI Y N, LOPES L D, et al. The effects of soil depth on the structure of microbial communities in agricultural soils in Iowa (United States)[J]. Applied and Environmental Microbiology, 2021, 87(4).
[32]	MUNDRA S, KJØNAAS O J, MORGADO L N, et al. Soil depth matters: shift in composition and inter-kingdom co-occurrence patterns of microorganisms in forest soils[J]. FEMS Microbiology Ecology, 2021, 97(3): fiab022.
[33]	HU L, WANG X, SONG X Y, et al. Vertical patterns of soil bacterial and fungal communities along a soil depth gradient in a natural picea crassifolia forest in Qinghai Province, China[J]. Forests, 2023, 14(5): 1016.
[34]	ZHANG S Y, ZHANG H F, LIU H M, et al. Combined organic and inorganic fertilization increases soil network complexity and multifunctionality in a 5-year fertilization system[J]. Land Degradation & Development, 2024, 35(2): 586-596.
[35]	TOLEDO S, PERI P L, CORREA O S, et al. Structure and function of soil microbial communities in fertile islands in austral drylands[J]. Austral Ecology, 2022, 47(3): 663-673.
[36]	SILBY M W, NICOLL J S, LEVY S B. Requirement of polyphosphate by pseudomonas fluorescens Pf0-1 for competitive fitness and heat tolerance in laboratory media and sterile soil[J]. Applied and Environmental Microbiology, 2009, 75(12): 3872-3881.
[37]	DAIMS H, LEBEDEVA E V, PJEVAC P, et al. Complete nitrification by nitrospira bacteria[J]. Nature, 2015, 528(7583): 504-509.
[38]	MA X M, ZHOU Z, CHEN J, et al. Long-term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil[J]. Science of the Total Environment, 2023, 854: 158709.
[39]	LUAN H A, GAO W, HUANG S W, et al. Substitution of manure for chemical fertilizer affects soil microbial community diversity, structure and function in greenhouse vegetable production systems[J]. PLoS One, 2020, 15(2): e0214041.
[40]	SUN R B, ZHANG X X, GUO X S, et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry, 2015, 88: 9-18.
[41]	LI Y L, TREMBLAY J, BAINARD L D, et al. Long-term effects of nitrogen and phosphorus fertilization on soil microbial community structure and function under continuous wheat production[J]. Environmental Microbiology, 2020, 22(3): 1066-1088.
[42]	CHEN M M, PAN H, SUN M J, et al. Nitrosospira cluster 3 lineage of AOB and nirK of Rhizobiales respectively dominated N₂O emissions from nitrification and denitrification in organic and chemical N fertilizer treated soils[J]. Ecological Indicators, 2021, 127: 107722.

处理	pH值	SOM含量/ (g·kg^-1)	TN含量/ (g·kg^-1)	TP含量/ (g·kg^-1)	TK含量/ (g·kg^-1)
CK20	5.45±0.03 c	10.30±0.38 c	0.55±0.35 d	0.37±0.04 e	17.31±0.61 c
Okitsu20	4.66±0.02 f	31.36±1.12 a	1.72±0.06 a	1.02±0.01 c	24.73±0.90 ab
Ponkan20	5.21±0.01 d	25.00±4.28 b	1.59±0.06 b	1.50±0.01 a	23.72±0.58 b
CK40	5.96±0.02 a	11.70±1.86 c	0.35±0.08 e	0.26±0.03 f	18.21±0.60 c
Okitsu40	4.74±0.04 e	32.00±2.08 a	1.35±0.06 c	0.95±0.01 d	25.78±0.58 a
Ponkan40	5.72±0.10 b	30.05±2.34 a	1.48±0.06 b	1.39±0.01 b	23.95±0.58 b

处理	pH值	SOM含量/ (g·kg^-1)	TN含量/ (g·kg^-1)	TP含量/ (g·kg^-1)	TK含量/ (g·kg^-1)
CK20	5.45±0.03 c	10.30±0.38 c	0.55±0.35 d	0.37±0.04 e	17.31±0.61 c
Okitsu20	4.66±0.02 f	31.36±1.12 a	1.72±0.06 a	1.02±0.01 c	24.73±0.90 ab
Ponkan20	5.21±0.01 d	25.00±4.28 b	1.59±0.06 b	1.50±0.01 a	23.72±0.58 b
CK40	5.96±0.02 a	11.70±1.86 c	0.35±0.08 e	0.26±0.03 f	18.21±0.60 c
Okitsu40	4.74±0.04 e	32.00±2.08 a	1.35±0.06 c	0.95±0.01 d	25.78±0.58 a
Ponkan40	5.72±0.10 b	30.05±2.34 a	1.48±0.06 b	1.39±0.01 b	23.95±0.58 b

处理	MBC/ (mg·kg^-1)	MBN/ (mg·kg^-1)	MBP/ (mg·kg^-1)
CK20	54.42±4.80 c	5.95±0.16 d	2.70±0.65 cd
Okitsu20	215.04±13.88 a	26.30±2.27 a	8.74±1.26 a
Ponkan20	130.29±42.38 b	14.40±1.08 b	5.47±1.77 b
CK40	32.05±4.63 c	3.60±1.24 d	1.80±0.07 d
Okitsu40	138.89±14.46 b	14.91±2.68 b	3.59±0.36 c
Ponkan40	56.11±5.87 c	10.57±1.08 c	3.23±1.06 cd

处理	MBC/ (mg·kg^-1)	MBN/ (mg·kg^-1)	MBP/ (mg·kg^-1)
CK20	54.42±4.80 c	5.95±0.16 d	2.70±0.65 cd
Okitsu20	215.04±13.88 a	26.30±2.27 a	8.74±1.26 a
Ponkan20	130.29±42.38 b	14.40±1.08 b	5.47±1.77 b
CK40	32.05±4.63 c	3.60±1.24 d	1.80±0.07 d
Okitsu40	138.89±14.46 b	14.91±2.68 b	3.59±0.36 c
Ponkan40	56.11±5.87 c	10.57±1.08 c	3.23±1.06 cd