Abstract:

Inter-subgroup hybridization between Paeonia delavayi and Japanese peony cultivars as parents is an important breeding pathway to cultivate excellent peony varieties, and early molecular characterization of the authenticity of their hybrid offspring can shorten the breeding cycle, improve the breeding efficiency, and reduce the breeding cost. In this study, two offspring seedlings were obtained by hand-pollination hybridization between Paeonia delavayi as the female parent and Japanese peony cultivar Daojin as the male parent. The authenticity of the hybrid offspring was identified using SSR molecular marker technology. The results revealed that two hybrid seedlings had parental specific complementary bands in two pairs of SSR primers, confirming that they were true hybrid species. The SSR molecular marker technology applied by this research institute has accurate and efficient characteristics, which can be used for early identification of peony hybrid offspring and provide technical support for cultivating excellent peony varieties.

Key words: peony inter-subgroup hybridization, offspring seedling, SSR molecular marker